Detection of Phaeocystis globosa using sandwich hybridization integrated with nuclease protection assay (NPA-SH)

Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China. In this study, sandwich hybridization integrated with nuclease protection assay (NPA-SH) was used to qualitatively and quantitatively detect P. globosa. Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields. The linear regression equation for P. globosa was obtained, and the lowest detection number of cells was 1.8×104 cells. Statistics showed that there was no distinct difference between the results of detecting the microalgae by NPA-SH and traditional microscopy. This technique has good reliability, accuracy, and can give a remarkably high sample processing rate. Sandwich hybridization integrated with nuclease protection assay will provide an efficient alternative to microscopic method for monitoring and investigating the bloom of P. globosa.     

Detection of Phaeocystis globosa using sandwich hybridization integrated with nuclease protection assay (NPA-SH)

Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China. In this study, sandwich hybridization integrated with nuclease protection assay (NPA-SH) was used to qualitatively and quantitatively detect P. globosa. Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields. The linear regression equation for P. globosa was obtained, and the lowest detection number of cells was 1.8 × 10⁴ cells. Statistics showed that there was no distinct difference between the results of detecting the microalgae by NPA-SH and traditional microscopy. This technique has good reliability, accuracy, and can give a remarkably high sample processing rate. Sandwich hybridization integrated with nuclease protection assay will provide an efficient alternative to microscopic method for monitoring and investigating the bloom of P. globosa.     

污泥热泵干燥系统的节能减排效益分析

定义污泥热泵干燥系统的能量效率,并通过过程能量组合分析,给出了能量效率的计算公式。分析结果表明:污泥热泵干燥系统的节能与减排效益呈现相反的变化规律,系统能量效率降低,但废气排放量减小,当能量效率为正值时,应用热泵可获得节能减排双重效益,但当能量效率为负值时,应用热泵获得的减排效益是否可弥补能量浪费应综合权衡。     

Impact analysis of multi-sensor layout on the source term estimation of hazardous gas leakage

In chemical industry, sensors are used to monitor the leakage and emission of hazardous materials that are used for hazard warning and risk assessment to ensure safety production. The traditional sensor layout designs the scheme at single-layer, and thus causes large deviations in the estimated height and accuracy of source term estimation (STE). In this study, a dual-layer layout scheme for sensors is proposed. The numerical experiments verify that the improved schemes with an equal number of sensors, as well as detection errors, are beneficial to the accuracy of the STE results. The influence of the heights of the sensors and leak source on the results of STE is studied. Results show that the dual-layer sensor scheme with adjacent intervals at high places in the potential search space is highly favorable to locate the leak, and the scheme arranged near the ground is conducive for improving the estimation accuracy of source intensity. This study also compares the STE results of computational fluid dynamics (CFD) simulated scenarios under different sensor schemes and verifies the effectiveness of the proposed dual-layer sensor deployment scheme with adjacent intervals under turbulence condition.     

生态系统健康评价新视角及其应用

对人类生存和发展所依赖的生态系统之健康性的讨论,迄今已引起多个学科的重视。到目前为止,对几乎所有的生态系统类型都各自进行过健康评价。但是,由于生态系统健康研究是一个新领域,有关它的概念、评价等方面存在各种争论,故急需一种更能被普遍接受的、用来定义、分析、评价生态系统健康的理论与方法。论文阐述了生态系统健康的内涵以及评价生态系统健康的基础前提,依据统计学理论,通过因子分析和正态分布理论,提出了评价生态系统健康的一个新视角。最后通过模拟实例对100个生态系统群进行生态系统健康评价,找到了其中不健康的11个生态系统。该方法能通用于各种类型的生态系统健康评价,为生态系统健康的定量分析与评价提供了新的途径。     

一体化联合作战后勤训练电磁环境构建方法研究

针对一体化联合作战后勤训练的特点,提出了可视化战场电磁环境和模拟战场电磁环境两种构建方法,并对模拟战场电磁环境的构建方法作了详细阐述。应坚持作战与训练的双重需求,强化军用与民用的优势互补,注重定性与定量的有机结合。     

基于遗传算法的综合热能管理系统代偿优化

在SIMULINK平台下搭建了飞机综合热能管理系统仿真模型,并利用遗传算法对其可控参数如发动机引气的阀门开度、冲压空气进口面积和液体环路的流量等进行在不同飞行状态下的寻优,使得飞机在满足温度等要求的情况下起飞总重的代偿较小。结果表明,利用智能算法可以实现这种复杂系统的参数优化,从而提高飞机燃油经济性。     

环境经济综合核算的环境资源价值问题的研究

随着人类社会经济的发展,环境污染日益严重以及环境资源的日趋短缺问题的出现,环境资源也逐渐显示出其稀缺性。在环境经济综合核算体系中,环境资源价值估算是环境核算的核心问题。环境资源价值问题的研究对资源管理、生态建设和环境保护具有重要的理论和现实意义。通过查阅国内外文献,探讨环境资源价值的研究现状、理论及其评估方法。在总结现有的环境资源价值评估方法的基础上,提出了应用能值分析方法来客观核算环境资源价值,从而能够更接近环境资源的真实价值。     

生态—地质环境承载力评价指标体系的探讨

生态—地质环境承载力评价是承载力研究的新课题,目前国内外都鲜见这方面的理论与实践研究案例。建立较为实用、具有一定普遍意义的生态-地质环境承载力评价指标体系则是生态-地质环境承载力科学评价的前提。本文以地球系统科学、生态学为理论依据,在环境承载力研究的基础上讨论了生态-地质环境承载力的概念、内涵,分析了生态-地质环境承载力的影响因素。以城市为研究背景,探讨了生态-地质环境评价指标体系的基本构成,建立了生态-地质环境承载力综合剩余率模型。该模型更科学地度量了区域人类活动与生态地质环境系统间的关系,为区域的可持续发展提供了理论依据。     

居民生活消费碳排放估算方法分析比较

对当前国内外居民生活消费碳排放估算方法进行对比,从居民生活消费能源的界定和估算方式的选择2方面入手,分析各种估算方法的优缺点,为我国合理准确的估算居民生活消费碳排放提供参考借鉴。