Agrobacterium-mediated transformation of Nicotiana attenuata, a model ecological expression system

Summary. Research into the genetic basis of the ecological sophistication of plants is hampered by the availability of transformable systems with a wealth of well-described ecological interactions. We present an Agrobacterium-mediated transformation system for the model ecological expression system, Nicotiana attenuata, a native tobacco that occupies the post-fire niche in the Great Basin Desert of North America. We describe a transformation vector and a transformation procedure that differs from the standard cultivated tobacco transformation protocols in its use of selectable markers, explants, media and cultivation conditions. We illustrate its utility in the transformations with genes coding for key enzymes in the oxylipin cascade (lipoxygenase, allene oxide synthase, hydroperoxide lyase) in antisense orientations and present high-throughput screens useful for the detection of altered phenotypes for the oxylipin cascade (green leaf volatiles and jasmonic acid after wounding). Received 14 March 2002; accepted 10 May 2002     

Constitutive or induced defences - how does <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> defend itself from larval feeding?

Summary. Following herbivory, induced responses involving plant secondary metabolites have been reported in a number of tree species. Although a wide range of plant secondary metabolites appear to operate as constitutive plant defences in trees belonging to the Eucalyptus genus, no induced responses have as yet been reported following foliar-chewing insect damage. We empirically tested whether branch defoliation (artificial and larval) of 2-year-old Eucalyptus globulus Labill. trees altered the abundance of specific plant secondary metabolites immediately (3 months after initial larval feeding) and 8 months after the cessation of larval feeding. Metabolites assayed, included essential oils, polyphenolic groups and foliar wax compounds and in all cases their abundance was not significantly altered by defoliation. However, the level of foliar tannins after 3 months of larval feeding did display a trend that suggested elevated levels as the result of defoliation, though this trend was not evident 8 months later, indicating that, if real, the response was a rapid and not a delayed induced response. The level of foliar tannins was also negatively correlated to both average larval survival and average percentage branch defoliation, suggesting that foliar tannins may operate as toxins and/or anti-feedants to M. privata larval feeding.     

An ecdysteroid (22-acetyl-20-hydroxyecdysone) from the defense gland secretion of an insect: <Emphasis Type="Italic">Chrysolina carnifex</Emphasis> (Coleoptera: Chrysomelidae)

Summary. 22-Acetyl-20-hydroxyecdysone was identified as a major component in the secretion of elytral and pronotal glands of Chrysolina carnifex, at an estimated concentration of 1.5 x 10^-1 M. This unexpected high concentration of an ecdysteroid in an exocrine secretion of an insect is discussed in terms of its potential defensive function, and in the light of steroid production in similar glands of closely related leaf beetles.     

“颗颗灵”矫治金川雪梨树缺铁黄叶病

本文采用树干深层埋植铁剂“颗颗灵”对金川雪梨缺铁黄叶病进行了同株异枝的对比试验，结果表明经过埋植处理的缺铁黄化叶１５天均匀复绿，叶绿素、叶厚、百叶重、百叶体积和百叶面积等叶片生理、生长指标比对照增加显著或极显著，黄化叶、褐变叶发病率和黄化、褐变指数等生理病害指标下降到０值。     

MODELING WATER BALANCE WITH THE ERHYM MODEL ON SOUTH TEXAS RANGELANDS1

ABSTRACT: Understanding the hydrologic processes of rangeland plant communities is essential to determine if water augmentation through shrub management is feasible. Vegetation manipulation studies are costly, difficult to accurately replicate, and often require more than 10 years to determine treatment effect on the water budget. If properly applied, hydrologic simulation models are an attractive alternative for assessing vegetation manipulation practices. The ERHYM-II model was evaluated to determine if it was capable of simulating the water balance for honey mesquite shrub clusters, grass interspaces, and bare soil in south Texas. The simulated water budget was within 2 percent of the measured evapotranspiration for the shrub clusters and grass interspaces. The model underestimated the number of runoff events and overestimated runoff volume for the grass interspace and shrub clusters. Simulated runoff was overestimated by approximately twofold for the grass interspace and threefold for the shrub clusters. Although simulated runoff was substantially overestimated, observed and simulated runoff only accounted for 3 to 6 percent of annual rainfall for the grass and shrub dominated areas, respectively. Simulated evapotranspiration was underestimated by 18 percent and soil water content was overestimated by 82 percent for the bare soil. The model underestimated evapotranspiration for the bare soil as a result of restricting evaporative losses to the first soil layer. Based on our analysis, the ERHYM-II model has the potential for simulating the annual water balance for semiarid rangeland plant communities where runoff and deep drainage are limited components of the water balance.     

Cu、Cd污染背景下加拿大一枝黄花叶水提液对莴苣种子萌发和生长的生态毒理效应

在重金属污染加重及多样化的背景下,入侵植物对本地植物种子萌发和生长的生态毒理效应可能发生改变甚至增强。因此,本文探究了Cu和Cd单污染及其复合污染下入侵植物加拿大一枝黄花叶水提液对其本地近缘种莴苣种子萌发和生长的生态毒理效应。结果表明:加拿大一枝黄花叶水提液处理及3种重金属污染类型和加拿大一枝黄花叶水提液复合处理均显著降低莴苣种子发芽指数,表明重金属和加拿大一枝黄花叶水提液均对莴苣的种子萌发和生长产生明显生态毒理效应。Cu和Cd复合污染下加拿大一枝黄花叶水提液对莴苣幼苗株高产生的生态毒理效应显著大于在Cu和Cd单污染下的生态毒理效应,而在Cu和Cd复合污染下加拿大一枝黄花叶水提液对莴苣幼苗发芽速度指数产生的生态毒理效应也大于在Cu和Cd单污染下的生态毒理效应(未达到显著水平)。这表明在Cu和Cd复合污染下加拿大一枝黄花叶水提液对莴苣种子萌发产生的生态毒理效应大于在Cu和Cd单污染下的生态毒理效应,即Cu和Cd复合污染可能对加拿大一枝黄花对本地植物种子萌发和生长产生的生态毒理效应具有一定的叠加效应。     

Transpiration rates of urban trees, Aesculus chinensis

Transpiration patterns of Aesculus chinensis in relation to explanatory variables in the microclimatic, air quality, and biological phenomena categories were measured in Beijing, China using the thermal dissipation method. The highest transpiration rate measured as the sap flux density of the trees took place from 10:00 am to 13:00 pm in the summer and the lowest was found during nighttime in the winter. To sort out co-linearity, principal component analysis and variation and hierarchical partitioning methods were employed in data analyses. The evaporative demand index (EDI) consisting of air temperature, soil temperature, total radiation, vapor pressure deficit, and atmospheric ozone (O₃), explained 68% and 80% of the hourly and daily variations of the tree transpiration, respectively. The independent and joint effects of EDI variables together with a three-variable joint effect exerted the greatest influences on the variance of transpiration rates. The independent effects of leaf area index and atmospheric O₃ and their combined effect exhibited minor yet significant influences on tree transpiration rates.     

Morphological and biological features of plants related to their adaptation to coastal habitats

The results of studies on the biomorphological features of 22 plant species growing on the southern coast of the Russian Far East provide evidence for the diversity of pathways of their adaptation to stressful habitat conditions. An analysis of the anatomical and mesostructural characteristics of their leaves has revealed representatives of euhalophytes, crinohalophytes, and glycohalophytes among these plants. Adaptation is achieved by means of halomorphosis, haloxerophytism, and development of some heliophilic features accounted for by species-specific manifestations of succulence, the presence of salt excreting trichomes, and thick pubescence. The similarity of these adaptations to those of desert plants is discussed. Along with plants characterized by a high germination rate, species that counterbalance a low efficiency of seed reproduction by active vegetative propagation have also successfully established themselves on the coast. Adaptation to coastal habitats is also achieved due to the diversity of life forms characterized mainly by tap root systems with laterally extending branch roots, creeping shoots, and a high vegetative mobility.     

Visible injury, crown condition, and growth responses of selected Italian forests in relation to ozone exposure

The impact of ozone on forest ecosystems in Italy is monitored within the CONECOFOR programme. Ozone levels are measured in 30 plots using passive samplers. Response parameters used are: crown condition (transparency), BAI (basal area increment), and visible symptoms on spontaneous vegetation. Levels of AOT40 are above the concentration-based critical level of 5 ppmh in all sites, but the evidence of impact on forest vegetation remains limited. Ozone is a predictor of crown transparency residuals in beech sites over two consecutive years, but the variance explained amounts to less than 10%. The relation between BAI reduction and ozone is even less certain. Transparency and BAI are more readily explainable in terms of ecological conditions of the site and climate fluctuations. The interpretation of visible symptoms is doubtful, and is conditioned by the prevailing ecological factors in the areas.     

Modeling Stream Shade: Riparian Buffer Height and Density as Important as Buffer Width1

DeWalle, David R., 2010. Modeling Stream Shade: Riparian Buffer Height and Density as Important as Buffer Width. Journal of the American Water Resources Association (JAWRA) 46(2):323-333. DOI: 10.1111/j.1752-1688.2010.00423.x Abstract: A theoretical model was developed to explore impacts of varying buffer zone characteristics on shading of small streams using a path-length form of Beer’s law to represent the transmission of direct beam solar radiation through vegetation. Impacts of varying buffer zone height, width, and radiation extinction coefficients (surrogate for buffer density) on shading were determined for E-W and N-S stream azimuths in infinitely long stream sections at 40°N on the summer solstice. Increases in buffer width produced little additional shading beyond buffer widths of 6-7 m for E-W streams due to shifts in solar beam pathway from the sides to the tops of the buffers. Buffers on the north bank of E-W streams produced 30% of daily shade, while the south-bank buffer produced 70% of total daily shade. For N-S streams an optimum buffer width was less-clearly defined, but a buffer width of about 18-20 m produced about 85-90% of total predicted shade. The model results supported past field studies showing buffer widths of 9-11 m were sufficient for stream temperature control. Regardless of stream azimuth, increases in buffer height and extinction coefficient (buffer density) were found to substantially increase shading up to the maximum tree height and stand density likely encountered in the field. Model results suggest that at least 80% shade on small streams up to 6-m wide can be achieved in mid-latitudes with relatively narrow 12-m wide buffers, regardless of stream azimuth, as long as buffers are tall (≈30 m) and dense (leaf area index ≈6). Although wide buffers may be preferred to provide other benefits, results suggest that increasing buffer widths beyond about 12 m will have a limited effect on stream shade at mid-latitudes and that greater emphasis should be placed on the creation of dense, tall buffers to maximize stream shading.