江苏省8个沿海造林树种的耐盐性评价研究

以江苏省盐城市大丰区沿海地区为研究区域,选择中山杉（Taxodium 'Zhongshanshan'）、白榆（Ulmus pumila）、落羽杉（Taxodium distichum）、榔榆（Ulmus parvifolia）、乌桕（Triadica sebifera）、青皮柳（Salix ohsidare）、构树（Broussonetia papyrifera）和苦楝（Melia azedarach）8个造林树种,研究不同树种造林地土壤含盐量及其与树木成活率、胸径和树高生长量的关系,并对8个树种的耐盐性进行隶属函数法综合评价,以进一步研究江苏沿海地区典型树种的耐盐性。结果表明,48个调查样地土壤含盐量为0.4~11.9 mg/g;不同样地之间的土壤含盐量差异较大;土壤含盐量较高的样地具有典型的盐分表聚现象;8个树种的成活率、胸径和树高年生长量与土壤含盐量均呈负相关。8个造林树种的耐盐性评价结果显示,其耐盐能力表现为白榆>苦楝>落羽杉>乌桕>榔榆>中山杉>青皮柳>构树。其中,白榆和苦楝可耐受6 mg/g的土壤含盐量;落羽杉、乌桕、榔榆和中山杉可耐受4 mg/g的土壤含盐量;青皮柳和构树只能耐受3 mg/g的土壤含盐量。白榆和苦楝可在含盐量较高的沿海地区造林,落羽杉、乌桕、榔榆和中山杉可在含盐量中等的沿海地区造林,青皮柳和构树只能在含盐量较低的沿海地区造林。

     

基于CFD的系留气球表面结冰研究

目的 预测不同环境条件下系留气球的结冰部位与结冰量,为系留气球的安全运行提供参考依据.方法 利用气候试验室对柔性材料制成的试验件进行结冰研究,并与金属表面结冰进行对比分析,明确柔性材料的结冰机理.采用数值模拟的方法,对某型系留气球进行多参数多状态结冰研究,同时开展系留气球结冰后的稳定性分析.结果 保持一定刚度的柔性材料和金属材料结冰情况基本一致,结冰机理相同.计算得出了通常结冰、冻雾和冻雨条件下,各俯仰角下系留气球表面的结冰分布情况.通常结冰条件下,结冰主要发生在尾翼前缘,结冰量随风速和俯仰角增大而增大;在冻雾和冻雨条件下,结冰部位则出现在囊体和尾翼,结冰量随风速和俯仰角呈V形变化.结论 依据计算结果开展稳定性分析得出,系留气球在剩余浮力正常的情况下具备一定的\"结冰容限\",但长时间处于恶劣天气时仍需开展冰雪应对措施.     

The continuing conundrum of the LEA proteins

Research into late embryogenesis abundant (LEA) proteins has been ongoing for more than 20 years but, although there is a strong association of LEA proteins with abiotic stress tolerance particularly dehydration and cold stress, for most of that time, their function has been entirely obscure. After their initial discovery in plant seeds, three major groups (numbered 1, 2 and 3) of LEA proteins have been described in a range of different plants and plant tissues. Homologues of groups 1 and 3 proteins have also been found in bacteria and in certain invertebrates. In this review, we present some new data, survey the biochemistry, biophysics and bioinformatics of the LEA proteins and highlight several possible functions. These include roles as antioxidants and as membrane and protein stabilisers during water stress, either by direct interaction or by acting as molecular shields. Along with other hydrophilic proteins and compatible solutes, LEA proteins might also serve as “space fillers” to prevent cellular collapse at low water activities. This multifunctional capacity of the LEA proteins is probably attributable in part to their structural plasticity, as they are largely lacking in secondary structure in the fully hydrated state, but can become more folded during water stress and/or through association with membrane surfaces. The challenge now facing researchers investigating these enigmatic proteins is to make sense of the various in vitro defined functions in the living cell: Are the LEA proteins truly multi-talented, or are they still just misunderstood?     

Variation in populations of the coastal dune building grassLeymus arenarius in Iceland revealed by endospermal prolamins

Variation in the endospermal protein patterns of seeds amongst and between inland and coastal populations of the dune building grassLeymus arenarius was examined in Iceland. Seeds were collected from six coastal populations and five inland populations in Iceland. Endospermal proteins (prolamins) of seeds were extracted with Tris-buffered 2-propanol (50%) and β-mercaptoethanol (0.5% v/v). We used 8% and 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to separate the prolamin protein bands, which were stained with Coomassie Brilliant Blue R. Coastal populations were differentiated from inland populations on the basis of high frequency of high molar weight (HMW) (110–150 kg mol⁻¹) prolamins. Coastal populations had significantly higher proportions of their seed with more than two HMW prolamins, than those of inland populations. Subtle differences were found among the HMW prolamins of coastal and inland populations indicating limited intrapopulation variation. The dynamic environment of sand dunes probably influences the genetic composition of these populations. The results suggest that seeds of the inland and coastal populations should be treated separately in sand dune reclamation and restoration works     

镉胁迫对吊兰生长与土壤酶活性的影响

选用观赏植物吊兰进行盆栽试验,研究了吊兰对Cd的耐性、高浓度Cd胁迫对土壤酶活性以及土壤有效态Cd含量的影响.结果表明,随着Cd浓度的不断提高,脲酶活性显著下降.过氧化氢酶和蔗糖酶活性在Cd浓度为10 mg·kg-1的时候均达到了顶峰,而土壤磷酸酶活性则在Cd浓度为50 mg·kg-1的时候最大.4种土壤酶对重金属的敏感顺序为:脲酶> 磷酸酶> 蔗糖酶>过氧化氢酶.吊兰对Cd具有很强的耐性,在1500 mg·kg-1 Cd胁迫浓度范围内,吊兰对Cd的耐性指数均大于50%.土壤有效态Cd与土壤Cd添加量和土壤酶活性呈显著相关性,可将土壤有效态Cd含量和土壤酶活性这两类指标作为镉污染土壤的评价指标.     

Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage

It is observed that ramie (Boehmeria nivea), an economic fiber crop, can establish and colonize metal-contaminated sites in China. Metal tolerance and accumulation by ramie originating from 13 metal-contaminated and 4 “clean” sites in China were compared under field and hydroponic conditions. All selected populations and germplasms displayed good growth performance under diverse metal-contaminated habitats; while growth responses, metal accumulation and tolerance were similar among the 8 populations and 2 germplasms when exposed to solutions containing elevated As, Cd, Pb, or Zn in the laboratory. These revealed that ramie possesses a certain degree of constitutional metal tolerance. To our knowledge, this is the first report of constitutional metal tolerance possessed by a fiber crop. Ramie can be considered as a good candidate for both fiber production and phytoremediation of sites contaminated by multi-metals, as it accumulates relative low metal concentrations, but possesses both high biomass and high economic value.     

商陆耐重金属Cd关键酶抗氧化酶的研究

研究商陆(Phytolacca americana L.)对Cd胁迫的抗氧化酶响应,对于揭示其耐Cd机制具有重要意义.商陆幼苗在200μmol/L或400 μmol/L CdCl2营养液中处理4 d,叶片活性氧自由基和MDA含量升高,膜透性增加,光合速率降低,表明Cd胁迫诱导产生氧化胁迫.随着Cd处理含量的提高和时间的...     

商陆和烟草对锰胁迫的抗氧化响应研究

研究植物的重金属累积能力对于植物修复和植物冶金意义重大.Mn超累积植物商陆(Phytolacca americana L.)的耐性/累积机制至今还不清楚,为了研究商陆在Mn胁迫下的抗氧化酶作用,将萌发6周的商陆和烟草幼苗分别放入1 mmol·L~(-1)或3 mmol·L~(-1)的1/2 Hoagland营养液处理4 d,实验表明商陆叶片光合速率降低幅度显著低于烟草,而烟草MDA含量和电解质渗漏量增加幅度大于商陆.如1 mmol·L~(-1) Mn处理4 d后,与对照相比,商陆和烟草的光合速率分别降低13.3%和75.5%;烟草MDA含量和电解质渗漏量分别增加347.3%和120.1%,而商陆的MDA含量和电解质渗漏量没有明显增加,表明Mn胁迫对超累积植物引起的氧化损伤明显低于非累积植物.随着Mn处理浓度的提高和时间的延长,2种植物叶片SOD和POD活性均迅速增加,其中商陆SOD活性的增加幅度大于烟草;另外,商陆CAT活性持续上升,而烟草CAT活性显著下降.如在1 mmol·L~(-1) Mn处理下,商陆SOD、POD和CAT活性分别提高161.1%、 111.3%和17.5%;烟草SOD和POD提高55.5%和206.0%,而CAT下降15.6%,表明商陆的抗氧化酶,特别是CAT,能够有效地清除Mn毒产生的自由基.这些结果说明抗氧化酶的抗氧化能力是重金属累积植物商陆的Mn耐性机制之一.     

水培和盆栽条件下碎米莎草对铀的耐受性及富集特征

碎米莎草(Cyperus iria L.)作为铀的超耐受植物,它的发现为探讨铀污染环境的植物修复和铀在植物体中的吸收和耐受机理提供了一种新的资源. 在Hoagland's营养液中加入铀标准溶液模拟铀污染水环境,以及直接在铀尾沙条件分析不同铀供应水平下碎米莎草对铀的耐受性能和富集特征. 结果表明:①碎米莎草对铀的富集特征依赖于生长介质中铀的供应水平和植物生长期等多重因素. 碎米莎草不同生长期各部位w(U)均高于生长介质中的铀供应水平,根系和地上部分w(U)最高可分别达(48 605±1 266)和(824±856)mg/kg. 根、茎和叶中w(U)基本随着铀供应水平的升高而增加,在开花期时较低,根系吸收的铀有1/10~1/5能转移至茎、叶、花籽部位,ρ(U)<250 mg/L的铀溶液对碎米莎草的生长无抑制作用. ②碎米莎草对铀尾沙具有很强的耐受性,但地上部分的富集系数在某些条件下可大于1;地上部分的w(U)在开花期相对较高,至成熟期时下降;在成熟期添加柠檬酸有助于碎米莎草根、茎和叶将铀转移至籽部位.