Evaluation of protective effects of sulforaphane on DNA damage caused by exposure to low levels of pesticide mixture using comet assay

The objective of the study was to evaluate the potential risk of DNA damage due to exposure to a mixture of the most widely used pesticides, namely endosulfan, chlorpyriphos and thiram at an environmentally relevant concentration (5 μM each) and the DNA protective capacity of sulforaphane (SFN) (10–30 μg/mL). DNA damage in human lymphocytes was ascertained with Single Cell Gel Electrophoresis (SCGE), also called Comet Assay. For positive control, H₂O₂ at 100 mM was used. The pesticide mixture produced DNA damage at the concentration used in the lymphocytes. SFN was able to offer a statistically significant (P < 0.01), concentration-dependant protection to DNA damage between 10–20 μg/mL in both the pre-incubation and co-incubation strategies. The results indicate that exposure to low levels of these pesticide mixtures can induce DNA damage, and the presence of SFN in diet may reduce the incidence of genetic damage, especially in farm workers. However, it is not clear whether SFN is involved in quenching of the free radicals generated by the pesticide mixture or it is involved in DNA repair mechanism.     

Effect of ambient-level gas-phase peroxides on foliar injury, growth, and net photosynthesis in Japanese radish (Raphanus sativus)

To investigate the effects of ambient-level gas-phase peroxides concurrent with O₃ on foliar injury, photosynthesis, and biomass in herbaceous plants, we exposed Japanese radish (Raphanus sativus) to clean air, 50 ppb O₃, 100 ppb O₃, and 2-3 ppb peroxides + 50 ppb O₃ in outdoor chambers. Compared with exposure to 100 ppb O₃, exposure to 2-3 ppb peroxides + 50 ppb O₃ induced greater damage in foliar injury, net photosynthetic rates and biomass; the pattern of foliar injury and the cause of net photosynthetic rate reduction also differed from those occurring with O₃ exposure alone. These results indicate for the first time that sub-ppb peroxides + 50 ppb O₃ can cause more severe damage to plants than 100 ppb O₃, and that not only O₃, but also peroxides, could be contributing to the herbaceous plant damage and forest decline observed in Japan's air-polluted urban and remote mountains areas.     

东北玉米低温冷害时空分布与多时间尺度变化规律分析

利用东北地区48个农业气象观测站1961-2010年逐日温度资料和近20多年玉米生育期资料,基于热量指数构建生育阶段冷害指数,采用EOF分析、Mann-Kendall法、子波变换等方法系统分析了近50年东北玉米4个生育阶段低温冷害时空分布及周期特征。结果表明:整个生育期全区平均冷害强度呈极显著的减弱趋势,地区间冷害变化趋势呈现差异化特征,冷害强度减弱趋势由西南向东北方向呈阶梯状递增;4个生育阶段冷害指数EOF第一模态均呈冷害空间变化趋势一致的特征;冷害强度呈较明显的减弱趋势,第1到第4生育阶段减弱趋势逐渐增大,突变均发生在1990年代中期;冷害强度具有较强的周期性,出苗—七叶、出苗—抽雄存在明显的23、25年周期,出苗—乳熟、出苗—成熟均具有较明显的3年周期振荡。     

混凝土大坝震害评估模型的研究及对黄河上游大坝的分析

利用前人所编的历代(共四代)地震烈度(地震动)区划图,组合叠加后得到一个综合烈度(地震动)。这种叠加是一个集成的专家系统综合认识结果,以此作为坝体可能遭受地震破坏危险的因素。初步研究建立了混凝土大坝震害评估模型,此模型是考虑了坝体的结构类型、建设年代、使用现状、场地条件、抗震设防水平等因素,用逐步线性拟合方法进行回归分析得到的。用此模型对汶川地震破坏的坝体进行了实例分析,分析结果比较符合实际。最后,用这个模型对黄河上游10座重点混凝土大坝做了震害评估分析。     

某军机整体壁板裂纹损伤强度评估研究

用全机有限元模型模拟损伤部位的局部位移和力边界条件,对机翼整体壁板高应力区的裂纹型损伤分析,研究了不同损伤尺寸对整体壁板强度的影响,给出损伤尺寸与残余过载、残余强度系数的关系,结论对外场评定战伤飞机损伤程度提供依据.     

郑州园林绿化树木低温寒冻害空间分布特征研究

绿化树木是园林景观的主体部分,同样也是园林建设中的目标植物.然而,绿化植物容易受到低温寒冻害的影响,进而影响园林布局美观.基于此,该文提出郑州园林绿化树木低温寒冻害空间分布特征研究方法,统计郑州园林绿化树木类型,对各样本按照乔木层与灌木层进行分类整理,将频度、显著度、密度等作为计算树木多样性的指标,分析比较结果,根据获...     

Thiram‐induced toxic liver injury in male sprague‐dawley rats

Abstract

A single i.p. dose (120 mg/kg) of thiram given to male Sprague‐Dawley rats caused a significant increase in the activity of SGOT and SGPT 24 hr post‐treatment indicating liver damage. A considerable diminution in the serum cholinesterase activity was also noted in the treated rats as against the control animals. Additional evidence for thiram‐induced liver toxicity is provided by the observation that there was approximately 50% inhibition of the activity of hepatic microsomal benzphetamine N‐demethylase with a concomitant decrease in the concentration of cytochrome P‐450, an important component of the mixed‐function oxidase system. Although not significant, hepatic glutathione levels were also depleted by thiram, probably making the liver susceptible to toxic injury.     

洪水灾害损失信息系统—FLOODIS系统设计

本文借助遥感技术，设计出一个专题地理信息系统一洪灾损失信息系统ＦＬＯＯＤＩＳ，为损失评估模型提供评估区域洪水灾害损失的各种自然地理和社会经济信息以及经系统内专家模块处理后的专题信息，同时对其计算结果进行多种方式输出。     

数据库语言在震害预测中的应用

震害预测的软件程序一般都是基于结构分析的原理，大都采用ＦＯＲＴＲＡＮ语言来编写，而震害预测工作中又大量用到分组和排序，这又是数据库语言的特长。本文介绍了在震害预测工作中应用数据库语言的一些体会，主要讨论了两种语言的接口问题。     

Integrated Assessment of Air Pollution Abatement Strategies in Urban Areas: Application to the Greater Athens Area

This paper presents an integrated system for the assessmentof technical and non-technical measures that are putforward in order to reduce air pollution levels in urbanareas. In contrast to the majority of the currentlyemployed assessment tools, this system allows for theevaluation of any proposed air pollution control measure interms of its combined impact on air quality and socialwelfare, by correlating the environmental and economicaspects of alternative air pollution abatement solutions.Based on the multi-pollutant, multi-effect concept, thesystem presented aims in providing policy-makers with areliable tool for the objective assessment of the mostcost-effective packages of measures, the latter beingallocated according to the particular features and needs ofthe areas examined.