微生物群落结构和多样性解析技术研究进展

微生物群落结构和多样性是微生物生态学和环境科学研究的热点内容，对于开发微生物资源，阐明微生物群落与其生境的关系，揭示群落结构与功能的联系，从而指导微生物群落功能的定向调控具有重要意义。基本按照年代顺序概述了常用的微生物群落结构及多样性解析技术，同时也体现了解析技术由片面向全面、由低分辨水平向高分辨水平的发展过程。20世纪70年代以前，对环境微生物群落结构及多样性的认识依赖传统的培养分离方法，方法的分辨水平低，认识是不全面的和有选择性的；20世纪70年代和80年代，在微生物化学成分分析的基础上建立了生物标记物方法(醌指纹法、磷脂脂肪酸法等)，对微生物群落结构和多样性的认识进入到较客观的层次上；在80和90年代，以DNA为目标物的现代分子生物学技术(rRNA基因测序技术、基因指纹图谱等)比较精确地揭示了微生物种类和遗传的多样性，并给出了关于群落结构的直观信息。指出了每种解析技术的功能特点和局限性，并展望了解析技术的发展趋势是原位、快速、灵敏、高通量和准确定量。     

双台子河口湿地植被修复生境健康评价方法研究

以2014—2015年双台子河口滨海湿地植被修复20个站位调查资料为基础,对湿地修复结构指标、功能指标和环境指标进行研究,结合湿地生态系统健康理论,构建湿地生态环境健康评价指标体系。运用层次分析法(AHP)确定权重,对各个指标赋予权重,将健康评价分为3个等级,确立了生态健康评价方法,对近岸破损湿地翅碱蓬修复生境进行评价。进一步引用评价净影响值(EI)方法判断生境修复前、后的健康对比状态,从而确定翅碱蓬对该破损湿地的修复效果显著。从景观生态学和健康评价角度分析来看,建立此评价方法是可行的,可为今后湿地的管理与保护提供科学依据。     

民机客舱安全疏散能力模糊综合评价

随着民航业高速发展,民航客机飞行事故和严重事故征候时有发生,并且很难完全避免,因此做好民机客舱安全疏散对减少人员伤亡损失有着重要意义。通过对影响民机客舱安全疏散的各种指标进行定性分析,建立民机客舱安全疏散能力评估指标集,应用层次分析法得到各指标的权重,建立多级模糊综合评价模型及安全评价等级模型。最后将此方法应用到实际案例中,得到某航空公司的客舱安全疏散能力评价结果,通过进一步分析并对该航空公司安全管理提出指导性建议。     

A new tool to map the major worldviews in the Netherlands and USA,and explore how they relate to climate change

For addressing climate change, public support for changes in policy is needed, as well changes in individual lifestyles. Both of these appear to be intimately related with people’s worldviews. Understanding these worldviews is therefore essential. In order to research and ‘map’ them, we translated the theoretical ‘Integrative Worldview Framework’ (IWF) into an empirical, quantitative approach. We constructed a worldview-scale aiming to distinguish between four major worldviews – labeled traditional, modern, postmodern, and integrative – and explored their interface with opinions and behaviors with respect to climate change. The survey was conducted with representative samples of citizens in the Netherlands and the USA (n = 527 and n = 556). The hypothesized worldviews were found in the data with a reasonable degree of reliability, especially in the Dutch sample. We also found consistent relationships between these worldview-clusters and a range of opinions, political priorities, and behaviors. In both countries postmoderns and integratives displayed significantly more concern about climate change as well as more sustainable behaviors, compared with moderns and traditionals. The implications of these findings for environmental policy and social science are noteworthy.     

建立我国生态环境标准体系的初步构想

我国的生态环境标准极其薄弱,建立生态环境标准体系是我国生态环境保护的迫切需要。借鉴我国目前针对污染防治的环境标准体系,提出了我国生态环境标准体系的基本框架,以及生态环境标准制订的原则和重点。     

Detection and quantitation of trace levels of low molecular weight halocarbons (LMHs) associated with commercial sodium humate

Examination of vapor above commercial sodium humate and above 10wt% aqueous humate solution by gas chromatography (GC) revealed the presence of chloroform and trichloroethylene. Further examination of the aqueous humate by the purge and trap procedure confirmed the association of chloroform and trichloroethylene with the humate. GC/mass spectrometry analyses of methylene chloride extract of the humate established the association of LMHs with the humate. Improved headspace capillary GC technique showed that the humate contained not only chloroform, trichloroethylene but several other LMHs in the range 190 to 4000ng·kg^‐1.