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1.
HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal de termination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 percent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases. 相似文献
2.
Utility of North Atlantic Right Whale Museum Specimens for Assessing Changes in Genetic Diversity 总被引:2,自引:0,他引:2
Howard C. Rosenbaum †§§ Mary G. Egan Phillip J. Clapham ‡ Robert L. Brownell JR. § Sobia Malik Moira W. Brown ††‡‡ Bradley N. White Peter Walsh † and Rob Desalle 《Conservation biology》2000,14(6):1837-1842
Abstract: We examined six historical specimens of the endangered North Atlantic right whale ( Eubalaena glacialis ) using DNA isolated from documented baleen plates from the late nineteenth and early twentieth centuries. Sequences from the mitochondrial DNA (mtDNA) control region from these samples were compared with those from a near-exhaustive survey (269 of approximately 320 individuals) of the remaining right whales in the western North Atlantic Ocean. Our results suggest that there has been only relatively modest change in maternal lineage diversity over the past century in the North Atlantic right whale population. Any significant reduction in genetic variation in the species most likely occurred prior to the late nineteenth century. One historical specimen was from the last documented female capable of propagating one of the maternal lineages in the population today; no females in the existing population have been found to carry this mtDNA haplotype. Analysis of the only specimens from the eastern North Atlantic right whale population ever to be examined revealed that eastern and western North Atlantic right whales may not have been genetically differentiated populations. Loss of gene diversity experienced by North Atlantic right whales over the last century has been modest, and the six decades of protection have been successful in maintaining much of the maternal lineage diversity that was present in the late nineteenth century. 相似文献
3.
MIRIAM H. INOUE RUBEM S. OLIVEIRA JR. JUSSARA B. REGITANO CÁSSIO A. TORMENA JAMIL CONSTANTIN VALDEMAR L. TORNISIELO 《Journal of environmental science and health. Part. B》2013,48(5):605-621
The objective of this study was to investigate the behavior of sorption and desorption of the herbicides atrazine (6-chloro-N 2-ethyl-N 4-isopropyl-1,3,5-triazine-2,4-diamine) and diuron [3-(3,4-dichlorophenyl)-1,1-dimethyleurea] in soil samples from a typical lithosequence located in the municipality of Mamborê (PR), southern Brazil. Five concentrations of 14C-atrazine and 14C-diuron were used for both herbicides (0.48, 0.96, 1.92, 3.84, and 7.69 mg L?1). Sorption of both herbicides correlated positively with the organic carbon and clay content of the soil samples. Sorption isotherms were well described by the Freundlich model. The slope values of the isotherm (N) ranged from 0.84 to 0.90 (atrazine) and from 0.75 to 0.79 (diuron) for the lithosequence samples. Sorption of diuron was high regardless of the soil texture or the concentration added. The desorption isotherms for atrazine and diuron showed good fit to the Freundlich equation (R 2 ≥ 0,87). Atrazine slope values for the desorption isotherms were similar for the different concentrations and were much lower than those observed for the sorption isotherms. Significant hysteresis was observed in the herbicide desorption. When the two herbicides were compared, it was found that diuron (N = 0.06–0.22) presented more pronounced hysteresis than atrazine. The results showed that, quantitatively, a greater atrazine fraction applied to these soils remains available to be leached in the soil profile, as compared to diuron. 相似文献
4.
C. E. Barthel JR. 《Journal of the Air & Waste Management Association (1995)》2013,63(11):475-478
This is a report on Meetings of Representatives of National Air Pollution Preventions Associations to Consider the Establishment of an International Union, held in Washington, D. C, June 18–19, 1964 and Houston, Texas, June 21–22, 1964. 相似文献
5.
H. H. Meredith JR 《Journal of the Air & Waste Management Association (1995)》2013,63(11):719-723
An American Petroleum Institute study of desulfurization of Caribbean fuel oil was completed in February 1967. The report is unique in that proprietary information was made available to the contractor, the Bechtel Corp., by task force members and licensors of desulfurization processes. Thus confidential information never before available to a single organization was used to develop feasibility and incremental costs of various processes for desulfurization. The report provides needed guideposts for decision-making by governmental units as well as by industry. Incremental costs are presented for intermediate levels of desulfurization from the normal level for Caribbean heavy fuel oil of 2.6% down to 0.5%, as well as the sensitivity of costs to sulfur credits and to rate of recovery of invested capital. Degree of justifiable confidence in the various data is discussed. Effects of desulfurization on other characterisics of the fuel oil are also discussed. 相似文献
6.
Relictual Amphibians and Old-Growth Forests 总被引:3,自引:0,他引:3
Abstract: Terrestrial and aquatic herpetofauna were sampled by pitfall traps, time-constrained searches, and area-constrained searches (stream sites only) over a three-year period to examine the importance of forest age to amphibians and reptiles. Fifty-four terrestrial and 39 aquatic sites in Douglas-fir—dominated, mixed evergreen forests were located in southwestern Oregon and northwestern California Mean age of trees on sites ranged in age from 30 to 560 years. Thirty-one species of amphibians and reptiles were detected from the 93 localities. Only three species were found primarily on older forest sites: the Del Norte salamander (Plethodon elongatus), the Olympic salamander (Rhyacotriton olympicus), and the tailed frog (Ascaphus truei). Paleoeco-logic evidence indicates an historical association between these three amphibians and the extant elements of ancient primeval coniferous forests of the Pacific Northwest. The life histories and habitat requirements of these species suggest that these forms are scarce in younger forests because the microclimatic and microhabitat conditions they require generally exist only in older forests. The long-term viability of these species in northern California and southern Oregon may depend upon developing forestry practices that protect these mitical microclimates and microhabitats. 相似文献
7.
Abstract: Starch-gel electrophoresis was used to screen 101 bison from Badlands National Park, South Dakota, for variation at 24 genetic loci. The population was descended from founder groups of about 6 and 3 individuals, separated geographically for a minimum of 64 years. The purpose of this study was (1) to estimate levels of genic variability in this bison population, (2) to assess the extent to which descendents of the two founder groups differ genetically, and (3) to compare the genetic characteristics of the Badlands population with other bison populations. The Badlands herd was found to be polymorphic for only a single locus (MDH–1). Descendents of the founder groups were homogeneous with respect to allelic and genotypic frequencies at this locus. The MDH–1 polymorphism has not been observed in other bison populations, while several polymorphism reported in other bison populations were not detected in the Badlands herd. A mean heterozygosity of 0.012 was observed in the Badlands herd; this value is lower than that typically reported for mammals, though not as low as heterozygosities seen in other populations that have passed through severe bottlenecks in size. These results underscore the need for genetic data in planning breeding programs for species in captivity or managed in isolate reserves. 相似文献
8.
Abstract: Success in conservation biology depends upon a synergistic combination of short-term tactics and long-term strategies. Although the former are often necessary to forestall immediate habitat losses, the latter provide the critical framework of understanding needed to develop effective short-term priorities. Although many conservation biologists now emphasize short-term tactics, prudence dictates the concomitant establishment of long-term research projects aimed at answering fundamental ecological questions.
One deterrent to amassing such long-term data bases, aside from time and funding, is a lack of suitable sites where such studies can be conducted on a large scale. We describe two established major land-holding networks in the United States that could serve as appropriate places to develop long-term studies: the National Science Foundation's Long-Term Ecological Research (LTER) sites, and the US. Department of Energy's National Environmental Research Parks (NERPs). Because they consist of established research facilities, both networks can provide conservation biologists with "low-cost" baseline information on ecological processes, as well as access to a number of representative terrestrial and aquatic habitats under more or less "controlled" conditions suitable for long-term studies. We recommend that conservation biologists explore the possibility of using these or similarly available sites in their research programs. 相似文献
One deterrent to amassing such long-term data bases, aside from time and funding, is a lack of suitable sites where such studies can be conducted on a large scale. We describe two established major land-holding networks in the United States that could serve as appropriate places to develop long-term studies: the National Science Foundation's Long-Term Ecological Research (LTER) sites, and the US. Department of Energy's National Environmental Research Parks (NERPs). Because they consist of established research facilities, both networks can provide conservation biologists with "low-cost" baseline information on ecological processes, as well as access to a number of representative terrestrial and aquatic habitats under more or less "controlled" conditions suitable for long-term studies. We recommend that conservation biologists explore the possibility of using these or similarly available sites in their research programs. 相似文献
9.
We sampled understory hummingbirds in Amazonian forest fragments from before isolation through nine years after isolation. We recorded 377 captures of eight species in five 1-ha fragments and four 10-ha fragments. The three species netted before isolation, Phaethornis superciliosus, Phaethornis bourcieri , and Thalurania furcata , were nearly equally abundant at that time. After isolation abundance of P. bourcieri and T. furcata did not change, but P. superciliosus became nearly twice as common. Five additional species that were netted only after isolation represented about 10% of the post-isolation sample. The species recorded only after isolation were forest species usually found above the levels of nets; fragments were not colonized by nonforest species. Use of fragments did not differ between 1- and 10-ha fragments. The landscape surrounding the fragments included active cattle pasture, abandoned pasture, and Cecropia -dominated second growth, but this variation had little effect on use of fragments by hummingbirds. The results suggest that these understory hummingbirds can persist in a matrix of fragments, secondary growth, and large forest patches. This response is much different than that of the insectivorous birds that dominate the understory bird community at the site, which are much more vulnerable to fragmentation. 相似文献
10.