Microbial Poly(L-Lactide)-Degrading Enzyme Induced by Amino Acids, Peptides, and Poly(L-Amino Acids)

Poly(L-lactide)(PLA)-degrading activities of a fungus, Tritirachium album, and two strains of actinomycetes,Lentzea waywayandensis and Amycolatopsis orientalis, were inducible by some proteins (poly-L-amino acid), peptides and amino acids. Extracellular PLA-degrading activity of the culture filtrates was detected when these strains grew in liquid basal medium containing 0.1% (w/v) of (poly-L-amino acids), peptides or amino acids as the enzyme inducer. In addition to PLA-degrading activity, succinyl-(L-alanyl-L-alanyl-L-alanine)-p-nitroanilide (Suc-(Ala)₃-pNA)-degrading activity was observed, implying that the enzymes produced were protease-type. The enzyme activities produced varied between different strains and different inducers. Silk fibroin was the best inducer for A. orientalis and that elastin was the best inducer for L. waywayandensis and T. album.     

Three-year monitoring results of nitrate and ammonium wet deposition in Thailand

Wet deposition is one of the important sources of nitrogen input into the ecosystem. It also contributes to rain acidity in some environments. In this study we reported the annual as well as seasonal trends of nitrogen wet deposition at three locations in Thailand: Bangkok, Chiang Mai and Nan. Comparison of nitrogen wet deposition between in rural and in the urban areas was also made. Daily rainfall was measured and monthly rainwater was collected for nitrogen analysis during 1999–2002. The average NO₃ ^– concentration in rainwater collected from the rural sites (60 km from urban area) was around 0.2–0.3 mg L^–1, while that from the urban areas of Chiang Mai and Nan cities it was 0.4–0.5 mg L^–1. NH₄ ⁺ concentration in rainwater showed the similar ranges to that of NO₃ ^– , except at Nan where concentration was not significantly different between the urban and rural sites. On the other hand, the average concentrations of NO₃ ^– were higher at Bangkok site than other sites, while concentration of NH₄ ⁺ was almost the same between Chiang Mai and Bangkok. Wet deposition of NO₃ ^– at the rural sites of Chiang Mai and Nan ranged from 2.1 to 3.2 kg N ha^–1 yr^–1, while at the urban sites this ranged from about 6 kg N ha^–1 yr^–1 in Chiang Mai and Nan Cities to 8.6 kg N ha^–1 yr^–1 in Bangkok. Wet deposition of NH₄ ⁺ at the rural sites of Chiang Mai and Nan was about 2.4 to 3.6 kg N ha^–1 yr^–1 and at the urban sites of Chiang Mai, Nan and Bangkok this was 7.7, 4.9 and 8.1 kg N ha^–1 yr^–1, respectively. Thus, it was concluded that wet deposition of both nitrogen species was significantly higher at the urban sites than at the rural sites.