Municipal Wastes Treatment and Production of Polyhydroxyalkanoate by Modified Two-Stage Batch Reactor

The municipal wastes were utilized as substrate for polyhydroxyalkanoate (PHA) using two strains of Bacillus licheniformis (PHAs-007, wild type and M2-12, mutant). Municipal wastes were subjected to separate wastewater and biosolid. Municipal biosolid was digested by anaerobic bacteria thereafter only the supernatant with soluble organic compounds was subjected into the PHA-producing reactor containing municipal wastewater. The mutant strain M2-12 gave the highest value of biomass (42.0 ± 2.0 g/L) and PHA concentration (37.4 ± 1.0 g/L with 88.9 % of dry cell weight, DCW) and reduced 76.5 % of soluble chemical oxygen demand after 60 h of cultivation. The value of pH, biochemical oxygen demand and total solid of the reclaimed wastewater after PHA recovery was 7.1, 20 and 97 mg/L, respectively. Moreover, the polymers produced by both strains of B. licheniformis were characterized. The resultant polymer from B. licheniformis PHAs-007 and M2-12 cultivated in the PHA-producing reactor was identified as poly-3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB-co-3HV)] and poly-3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], respectively. The results suggesting that the production of PHA by municipal wastes is feasible thus the PHA production stage can be integrated in waste treatment to produce PHA and treated municipal wastes at the same time.     

A Novel Green Process for Synthesis of 3-Hydroxyalkanoate Methyl Ester Using Lipase and Novel mcl-co-lcl PHA as Catalyst and Substrate

Journal of Polymers and the Environment - A new type of medium-chain-length-co-long-chain-length polyhydroxyalkanoate (mcl-co-lcl PHA) containing 39.25 mol% 3-hydroxyhexadecanoic (3HHD)...     

An Environmentally Friendly Process for Textile Wastewater Treatment with a Medium-Chain-Length Polyhydroxyalkanoate Film

Journal of Polymers and the Environment - In this study, a novel medium-chain-length polyhydroxyalkanoate (mcl-PHA) film containing 39.25 mol% 3-hydroxyhexadecanoic acid...     

Screening and Identification of Newly Isolated Basic Red 9-Degrading Bacteria from Textile Wastewater and Their Ability to Produce Medium-Co-Long-Chain-Length Polyhydroxyalkanoates

Journal of Polymers and the Environment - Newly isolated Basic Red 9-degrading bacteria were isolated from textile wastewater using a pretreatment method. Nine strains were isolated; however, only...