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HAO Chun-bo YAN Jun QU Meng-meng WANG Dong CHENG Shu-pei GU Ji-dong QIU Wan-fei WANG Yin-yin 《环境科学学报(英文版)》2003,15(5):590-594
There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs( genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnpl, mnp2, lipl, lip2, FLOI and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineefing technique to create the soecific functional stain for the nollution control. 相似文献
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Liu Feng Yu Yin-Yin Li Li-Juan Zeng Lan 《Journal of Material Cycles and Waste Management》2018,20(2):995-1005
Journal of Material Cycles and Waste Management - Recycled aggregates recovered from abandoned buildings or demolished concrete structures were reused to produce concrete columns as new building... 相似文献
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