Use of the chemical cleavage of mismatch method for prenatal deficiency diagnosis of alpha-1-antitrypsin

The most common mutation in alpha-1-antitrypsin deficiency, conversion of a G to an A at base 9989 (PI-Z), was detected with the chemical cleavage of mismatch method, demonstrating the power of the method for prenatal diagnosis. Exon V of the gene was amplified using the polymerase chain reaction and heteroduplexes were formed to test for the presence of the mutation. The predicted C mismatch was readily detectable with hydroxylamine, and by making the probe from the chorionic villus sample it was possible to determine that the fetus was heterozygous, not homozygous, for the mutation.     

Mitigation of odor causing emissions—Bench-scale investigation

Emissions of malodors are considered to be the greatest threat to the compost industry. In work presented here, several simple odor mitigation alternatives were investigated for their effectiveness in preventing the release of common odorants, such as terpenes, ammonia, and reduced sulfur compounds. The mitigation methods studied included the use of a blanket of finished compost, compost amendment mixed within the feedstock, odor neutralizing agents (ONAs), and oxygen release compounds (ORCs). Among the mitigation alternatives investigated in this study, the use of finished compost as a blanket and finished compost as an amendment yielded the most conclusive and significant results. Both of these alternatives yielded a substantial emission reduction for terpenes, ammonia, and reduced sulfur compounds. The application of finished compost blanket resulted in up to 95% reduction of terpene and 25% reduction of ammonia emissions. Blending the feedstock with finished compost also provided substantial reduction of terpene emissions ranging from 73.6 to 93.1% at the 24% blending ratio, and up to 85% ammonia reduction a the 35% blending ratio. Use of finished compost also provided 75% lower reduced sulfur compound emissions at the 12% blending ratio. Misting and application of odor neutralizing agents did not result in any consistent reduction in emissions for any of the odorous compounds tested.

Implications The odor emissions from composting are often considered to be the biggest threat to composting facilities. Because most facilities cannot afford enclosures and contained composting vessels, there is a need to inexpensively and effectively control the odor emissions from composting facilities. The findings of this research can lead the way for efforts to control odor easily and cost effectively. In fact, the application of a compost blanket for odor control is already gaining acceptance by the composting industry.     

Risk-mitigating beliefs,risk estimates,and self-reported speeding in a sample of Australian drivers

PROBLEM: Research suggests that people who engage in risk-taking behaviors often hold specific beliefs that can mitigate or reduce their perceptions of risk associated with those behaviors. METHOD: A scale was developed (Speeding Risk Belief Scale (SRBS)) to assess beliefs about speeding-related risk and predict self-reported speeding in a random-digit telephone survey of 800 South Australian drivers between the ages of 16 and 50. RESULTS: The scale was internally consistent, and path analyses showed it to be associated with self-reported speeding, both directly and indirectly through participants' estimates of speeding-related risk. DISCUSSION: Origins of risk-mitigating beliefs and the extent to which they may be causally linked with speeding are discussed, and recommendations are made for future research. IMPACT ON INDUSTRY: This research has strong implications for the conduct of countermeasure campaigns that disseminate information on speeding-related risk.     

棉田种植苘麻诱集B型烟粉虱机制研究

利用苘麻(Abutilon theophrasti Medic)的诱集作用是防治棉田烟粉虱的有效手段,本文报道了关于苘麻和棉花叶片颜色、气味、叶毛密度、细胞液pH值及营养等影响诱集作用因子的差异研究结果.试验结果表明,苘麻叶片的叶毛密度(197.7根/cm2)和棉花叶片(19.6根/cm2)有显著的差别;苘麻叶片在波长560 nm 下的光反射率达到0.2,显著高于棉花叶片(0.09).此外,苘麻叶片细胞液的pH值在整个生长季节均较棉花维持在一个较高的水平(5.8～6.2).研究还表明,烟粉虱成虫对苘麻叶片颜色和挥发性气味表现出正趋性,但与棉花相比较差异不显著;苘麻叶片中可溶性蛋白质、可溶性糖和总氨基酸的含量与棉花没有显著的差别.因此,苘麻植株叶片较高的叶毛密度、光反射率和细胞液pH值可能是引起烟粉虱强选择性的主要原因.     

Fetal cells in the uterine cervix: a source for early non-invasive prenatal diagnosis

Various non-invasive techniques for prenatal diagnosis have been under investigation. We evaluated the success of fetal sexing using a non-invasive technique for obtaining fetal cells, uterine cervix brushing, in combination with FISH. Thirty pregnant women who completed between 6 and 10 weeks of gestation and who were scheduled to undergo pregnancy termination were included in the study. A Pap smear cytobrush was inserted through the external os to a maximum depth of 2 cm and removed while rotating it a full turn. The material that was caught on the brush was spread on four microscope slides. Two-color FISH was used for fetal sexing. Following pregnancy termination, a placental sample was used for full karyotyping. In 29/30 cases FISH diagnosis was confirmed by chromosomal analysis. The only male case in which a Y chromosome was not seen was from a pregnancy of 6 weeks 6 days gestational age. One case was mosaic of 46,XY/47,XXY (25%). In most cases (7/13) the Y chromosome was already identified in the first analyzed slide. With the use of a cytobrush fetal cells can be easily obtained for the purpose of prenatal diagnosis of chromosomal disorders. Copyright © 2001 John Wiley & Sons, Ltd.     

Key actor perspectives on stakeholder engagement in Omani Environmental Impact Assessment: an application of Q-Methodology

Stakeholder engagement (SE), particularly with representatives of locally affected communities, is integral to Environmental Impact Assessment (EIA) processes, so critical evaluation of SE is necessary across a range of different socio-political contexts. EIA SE practice in the Sultanate of Oman is examined using Q-Methodology, a qualitative-quantitative discourse analysis technique, in order to evaluate key-actor perspectives and policy directions. Four discourses emerge, pertaining to (1) the institutionalisation of SE; (2) business as usual; (3) rights-centred engagement; and (4) decentralisation of EIA institutions. Consensus emerges that shows support for transparency and formalisation of SE; greater citizen-centred decision-making power; transparency in government guidelines; and the elimination of developer-led consultation processes. Policy options for reforming EIA policy are discussed, including a code of participation practice and a toolkit of suitable engagement methods.