Monitoring of Phenol in Wastewater Bioremediation by HPLC

Bioremediation emphasizes the detoxification and destruction of toxic substances by microorganisms. Wastewater obtained from an industrial concern was solvent extracted with methyl alcohol and dichloromethane and analysed by GC/MS. Besides phenol, a large variety of organic compounds were detected. Under controlled laboratory conditions, the wastewater was innoculated with a mixed culture of microorganisms specially selected for their abilities to degrade phenol. Samples were collected at regular intervals from the stirred tank bioreactor and analysed for phenol by reverse phase HPLC with a C18 column. Results shows that from an initial phenol concentration of 987 ppm, slightly more than 50% was destroyed within 163 hours. The dry weight of the microorganisms and the plate count (CFU/ml) shows a steady increase from 0.5238 gms to 0.5355 gms and from 1.1E+9 to 1.94E+13 respectively over the same period. This suggested that the phenol was consumed by the microorganisms as the sole carbon source.     

Integrated Land and Water Management in the United Kingdom: Narrowing the Implementation Gap

Riparian buffer zones have been incorporated in land and water management policy for England since 1994, when the Ministry of Agriculture, Fisheries and Food introduced a Water Fringe Option (WFO) as part of a broader habitat conservation scheme. Whilst natural scientists have examined the functioning of riparian buffer zones, understanding of farmers' decision making regarding the adoption or non-adoption of voluntary buffer zone policies is very limited. This paper examines the factors influencing the decision making of farmers who were eligible to join the WFO in three river catchments. Quantitative and qualitative information was collected from farmers using semi-structured interviews and was supplemented with in-depth interviews with representatives of public agencies, agricultural groups and independent experts. Data analysis was completed using the 'Framework' analytical approach and the Statistical Package for the Social Sciences 8.0 computer software. The research revealed that decisions to participate in the WFO were influenced by a mix of situational, psychological and sociological characteristics, which suggests that policy makers must attach greater importance to implementation conditions and farmers decision making if riparian buffer zones are to play a more prominent role in the management of land and water in rural catchments. Tightly structured schemes will only appeal to a narrow segment of the farming population and will not lead to widespread re-creation of riparian habitats. A more flexible and collaborative style of policy development is needed in order for riparian buffer zone policies to meet the circumstances and needs of the diverse UK farming community.     

Field-Based Evaluation Tool for Riparian Buffer Zones in Agricultural Catchments

Riparian buffer zones can improve water quality and enhance habitat, but a comprehensive yet rapid method that can assist the resource manager in assessing the effectiveness of buffers is not available. The aim of this paper is to describe and illustrate the use of a newly developed field-based evaluation tool for riparian buffer zones in agricultural catchments. The Buffer Zone Inventory and Evaluation Form (BZIEF) incorporates criteria-based scoring systems developed from literature review, subsequent peer-review, and then a pilot field study. Use of the BZIEF is demonstrated by comparing buffer zones in three catchments established for water quality and habitat improvement under the Water Fringe Option agrienvironment scheme in England in order to assess whether the buffers were likely to provide environmental enhancement. Results among the three catchments were generally similar; buffer zones scored highly for their abundant vegetation cover, lack of erosion, stream habitat quality, and sufficient width. Furthermore, previous grassland or arable land use did not substantially affect buffer zone ratings. However, the BZIEF indicated that inappropriate soil characteristics in one catchment were likely to constrain buffer zone effectiveness for improving water quality. In another catchment, poor riparian vegetation diversity and structure may yield ineffective habitat enhancement, according to the BZIEF. It was concluded that the BZIEF might be a useful tool for buffer zone comparison and monitoring, even though more work is needed to test and validate the method. For example, the BZIEF could be used to target appropriate locations for buffer zones and is flexible, so could be adapted for different policies, objectives and regions.