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1.
Stin OC Carnahan A Singh R Powell J Furuno JP Dorsey A Silbergeld E Williams HN Morris JG 《Environmental monitoring and assessment》2003,81(1-3):327-336
Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish. 相似文献
2.
Ellen Sidransky Susan H. Black Dawn M. Soenksen Shirley L. Jones Andrew D. Dorfmann Joseph D. Schulman MD 《黑龙江环境通报》1990,10(9):583-586
Chorionic villus sampling (CVS) with either transcervical catheters or transabdominal needles is a widely-accepted method for prenatal diagnosis. However, there exists a small subset of patients in whom sampling is difficult or impossible with either route because of individual anatomic variations. A new method of chorionic villus biopsy has been developed to circumvent these problems, utilizing transvaginal chorionic needle aspiration guided by an intravaginal ultrasound probe. This technique was performed successfully in 15 patients in whom villi could not be obtained by either of the conventional methods. This method now makes CVS possible in essentially all women regardless of their uterine anatomy or placental placement; it may also prove useful for very early chorionic sampling. 相似文献
3.
The Science of Nature - 相似文献
4.
5.
Harold N. Bass Joan B. Oliver Murali Srinivasan Ruth Petrucha Won Ng Jo Ellen S. Lee 《黑龙江环境通报》1986,6(1):33-35
Intrauterine fetal demise (IUFD) in one of twins at 12 weeks of gestation was accompanied by markedly elevated maternal serum alpha-fetoprotein (AFP) at 17 and 18 weeks. Amniotic fluid AFP from the healthy surviving twin's sac at 18·5 and 23 weeks was also greatly increased along with a positive acetylcholinesterase (AChE) band. Persistently elevated AFP and positive AChE so long after fetal demise–-6·5 and 11 weeks post IUFD–-has not, to our knowledge, been previously described. In similar cases, high level ultrasound and careful placental examination at birth should be utilized to search for fetal abnormalities or multiple pregnancy with IUFD. 相似文献
6.
Ellen M. Douglas Paul H. Kirshen Michael Paolisso Chris Watson Jack Wiggin Ashley Enrici Matthias Ruth 《Mitigation and Adaptation Strategies for Global Change》2012,17(5):537-562
We explored the possible future impacts of increased coastal flooding due to sea level rise and the potential adaptation responses
of two urban, environmental justice communities in the metropolitan Boston area of Massachusetts. East Boston is predominantly
a residential area with some industrial and commercial activities, particularly along the coastal fringe. Everett, a city
to the north of Boston, has a diversified industrial and commercial base. While these two communities have similar socioeconomic
characteristics, they differ substantially in the extent to which residents would be impacted by increased coastal flooding.
In East Boston, a large portion of residents would be flooded, while in Everett, it is the commercial/industrial districts
that are primarily vulnerable. Through a series of workshops with residents in each community, we found that the target populations
do not have an adaptation perspective or knowledge of any resources that could assist them in this challenge. Furthermore,
they do not feel included in the planning processes within their communities. However, a common incentive for both communities
was an intense commitment to their communities and an eagerness to learn more and become actively engaged in decisions regarding
climate change adaptation. The lessons that can be applied to other studies include 1) images are powerful tools in communicating
concepts, 2) understanding existing cultural knowledge and values in adaptation planning is essential to the planning process
and 3) engaging local residents at the beginning of the process can create important educational opportunities and develop
trust and consensus that is necessary for moving from concept to implementation. 相似文献
7.
TUGAI: An Integrated Simulation Tool for Ecological Assessment of Alternative Water Management Strategies in a Degraded River Delta 总被引:1,自引:0,他引:1
Schlüter M Rüger N Savitsky AG Novikova NM Matthies M Lieth H 《Environmental management》2006,38(4):638-653
The development of ecologically sound water allocation strategies that account for the needs of riverine ecosystems is a pressing
issue, especially in semiarid river basins. In the Aral Sea Basin, a search for strategies to mitigate ecological and socioeconomic
deterioration has been in process since the early 1990s. The Geographic Information System–based simulation tool TUGAI has
been developed to support the policy determination process by providing a simple, problem-oriented method to assess ecological
effects of alternative water management strategies for the Amudarya River. It combines a multiobjective water allocation model
with simple, spatially explicit statistical and rule-based models of landscape dynamics. Changes in environmental conditions
are evaluated by a fuzzy habitat suitability index for Populus euphratica, which is the dominant species of the characteristic riverine Tugai forests. Water management scenarios can be developed
by altering spatiotemporal water distribution in the delta area or the amount of water inflow into the delta. Outcomes of
scenario analysis are qualitative comparisons of the ecological effects of different options for a time period of up to 28
years. The given approach utilizes different types of knowledge, from quantitative hydrological data to qualitative local
expert knowledge. The main purpose of the tool is to integrate the knowledge in a comprehensive way to make it available for
discussions on alternative policies in moderated workshops with stakeholders. In this article, the modules of the tool, their
integration, and three hypothetical scenarios are presented. Based on the experience gained when developing the TUGAI tool,
we propose that the general framework can be transferred to other areas where tradeoffs in water allocation between the environment
and other water users are of major concern. The potential for a simulation tool to structure and inform a complex resource
management situation by involving local experts and stakeholders in the development of possible future scenarios will become
increasingly valuable for transparent and participatory resource management. 相似文献
8.
Michael Duijn Arwin van Buuren Magnus Sparrevik Adriaan Slob Gerald Jan Ellen Amy Oen 《Journal of Environmental Planning and Management》2016,59(5):927-947
This study aims at describing, analyzing and evaluating the relation between management styles and process dynamics of a complex planning process confronted with unexpected dynamics. The development of an aquatic disposal site for dredged contaminated sediments in Oslo was managed by a project management style focused on timely and cost-effective implementation. Coupling the remediation project with another infrastructural project and the actual construction of the site led to unexpected dynamics in terms of resistance and controversy. Project management had difficulties in adjusting its style accordingly, resulting in even more delay and resistance. Managing complex planning projects requires a style suitable to the characteristics of the project and the capability of adjusting it to changing circumstances. The paper concludes with some explanations why it is difficult to change management styles in complex planning and implementation processes and complexity-embracing approaches to deal with this. 相似文献
9.
Ellen Elizabeth Moore 《Environmental Communication: A Journal of Nature and Culture》2016,10(5):539-555
This paper examines ideologies about nature and the environment in popular, animated Hollywood films—including The Lorax, Wall-E, and Ice Age 2—through a symptomatic reading. The primary goal of the analysis is to elucidate key omissions in these texts through an assessment of the problematic—defined in this research as an a-priori answer to perceived audience concerns regarding the role of consumerism and corporate culture in environmental problems. Silences in the films revolve around: how environmental problems are defined; what the consequences are; who the responsible parties are; and what potential solutions exist to mitigate them. The significance of the research is underscored by the formation of an increasingly intimate relationship between children, consumer culture, and commercial media in the USA, as well as the increasingly dire information emerging about global environmental issues. This analysis reveals the dual, often conflicting, messages that commercial film provides for its young audiences about pivotal environmental problems and their potential resolution. 相似文献
10.
An indispensable asset at risk: merits and needs of chemicals-related environmental sciences 总被引:1,自引:1,他引:0