Glyphosate concentrations in global freshwaters: are aquatic organisms at risk?

Environmental Science and Pollution Research - Glyphosate is the most used herbicide worldwide. Many studies have reported glyphosate risks to aquatic organisms of different trophic levels....     

Accumulation of arsenic, lead, copper, and zinc, and synthesis of phytochelatins by indigenous plants of a mining impacted area

Several native plants, able to grow in an unconfined mining impacted area that is now in close vicinity with urban areas, were evaluated for their ability to accumulate heavy metals. The main soil contaminants were As, Pb, Cu, and Zn. Sampling of the rhizospheric metal polluted soil showed that Euphorbia prostrata Aiton, Parthenium incanum Kunth, and Zinnia acerosa (DC.) A. Gray were able to grow in the presence of high amounts of mixtures of these elements. The plants accumulated the metals in the above ground parts and increased the synthesis of thiol molecules. E. prostrata showed the highest capacity for accumulation of the mixture of elements (588 μg g DW^?1). Analysis of the thiol-molecules profile showed that these plants synthesized high amounts of long-chain phytochelatins, accompanied by low amounts of monothiol molecules, which may be related to their higher resistance to As and heavy metals. The three plants showed translocation factors from roots to leaves >1 for As, Pb, Cu, and Zn. Thus, by periodically removing aerial parts, these plants could be useful for the phytoremediation of semi-arid and arid mining impacted areas, in which metal hyper-accumulator plants are not able to grow.     

User experience and operational feasibility of four point-of-collection oral fluid drug-testing devices according to Brazilian traffic agents

Objective: Traffic fatalities in Brazil still rank among the highest worldwide, with an overall rate of 23.4 deaths/100,000 inhabitants/year. Although alcohol and drug use play an important role in traffic accidents, national data about their relative influence are scarce. Drug screening is not routinely performed by traffic agents because alcohol is the only substance regularly investigated in roadblocks. Therefore, we aimed to describe the initial traffic agent user experience for 4 handheld point-of-collection oral fluid drug testing devices used in routine roadblocks in Brazil, focusing on usage perceptions in hopes of generalizing this approach for other developing countries.

Methods: Four different oral fluid collection devices were evaluated: The DDS2, the DOA MultiScreen, the Dräger DrugTest 5000, and the Multi-Drug Multi-Line Twist Screen Device. Fourteen trained traffic agents obtained oral fluid from 164 drivers and performed 37 qualitative evaluations of the devices. Traffic agents filled out a questionnaire focusing on 9 feasibility criteria: Overall simplicity for roadside operation; operational success; saliva sample collection time; sample analysis time; ease of sample preparation and analysis; agreement with observed clinical signs; overall hygiene and safety; sufficient operating instructions; and hygiene of saliva collection. These were weighted based on an expert panel and yielded an overall composite device experience score that ranged from 1 (poor) to 100 (excellent).

Results: Ease of use, operational success, and acceptable collection and analysis time were considered the most important criteria by the expert panel. The results ranged from 27.3 to 88.9% for simplicity of use; 45.5 to 100.0% for operational success; 27.3 to 100% for acceptable collection time; and 36.4 to 100.0% for acceptable analysis time. The final device scores, based on the agents’ user experiences, ranked as follows: DOA MultiScreen: 49.3/100; Dräger DrugTest 5000: 82.4/100; Multi-Drug Multi-Line Twist Screen Device: 84.3/100; DDS2: 88.4/100.

Conclusion: Based on the selected criteria, 3 of the 4 devices were considered useful by traffic agents in routine roadblock operations. The weighted evaluations suggest that their ease of use (handling, sampling analysis, and reliability), as well as their agreement with findings obtained by other means, defined their utility to traffic agents, although such appraisals must be further analyzed in future studies.     

Assessment of Water Quality in a Border Region Between the Atlantic Forest and an Urbanised Area in Rio de Janeiro,Brazil

The preservation of water resources is one of the goals of the designation of parks that act as natural reservoirs. In order to assess the impact of the presence of humans in an environmental preservation area bordering urban areas, the presence of four pathogenic enteric viruses [group A rotavirus (RV-A), norovirus (NoV), human adenoviruses (HAdV), and hepatitis A virus (HAV)], as well as the physico-chemical parameters, and Escherichia coli levels were assessed in riverine water samples. From June 2008 to May 2009, monthly monitoring was performed along the Engenho Novo River. RV-A, NoV, and HAdV were observed in 29 % (31/108) of the water samples, with concentrations of up to 10³ genome copies/liter. The natural occurrence of infectious HAdV was demonstrated by Integrated Cell Culture-PCR (ICC-PCR). This study confirms the suitability of using the detection of fecal-oral transmitted viruses as a marker of human fecal contamination in water matrices and indicates the spread of pathogenic viruses occurring in an alleged area of environmental protection.     

Total mercury content found in edible tissues of top predator fish from the Gulf of California,Mexico

Fish at the top of the food chain bioaccumulate and biomagnify toxic metals including mercury (Hg), at a higher rate than nonpredatory fish. However, although some top predator fish species are important in the human diet, the risks for consumers in Mexico are difficult to evaluate due to the scarce baseline information available. In the present article, data on the total mercury (THg) concentrations found in edible tissues of different species of sharks, rays, large pelagic fishes and groupers from the Gulf of California are presented and compared with national and international health guidelines. During 2003 and 2004, 73 shark dorsal tissue samples, 52 ray samples, 66 large pelagic fish samples and 16 grouper samples were collected at different sites along the Gulf of California. Samples were digested in a microwave system and analyzed by cold vapor atomic absorption spectrophotometry. Thirty samples (14%) showed concentrations of Hg above the national statutory limit of 1?mg?kg^?1, from which 25 samples were sharks and 3 were large pelagic fishes. A positive correlation was found between the natural log of THg [ln(THg)] and total length (TL cm) in species of sharks, rays and groupers. The Teacapán estuary in Sinaloa was detected as a possible source of Hg into the Gulf of California due to historic mining in the watershed. Mean Hg concentrations exceeded the regulatory limit of 1?mg?kg^?1 in the following highly commercial shark species: smooth hammerhead, pelagic thrasher, pacific sharpnose shark, dusky shark, scalloped hammerhead, and whitenose shark, in that order. It is recommended that preventive food advisories be issued with respect to these species until further investigations are conducted.     

Evaluation of beach quality as perceived by users

Recreational beaches are strategic ecosystems for tourism and should be used in a sustainable manner. We studied three beaches in the municipality of Guaymas (NW Mexico), in order to assess their beach quality and identify key management issues. The evaluation was based on the perceptions of users concerning: (1) the user profile; (2) the recreational habits of users; and (3) the biophysical characteristics, infrastructure, services, and cleanliness of each beach. The results showed that the beaches were of different quality. The key management issues identified were the need to design and apply specific management programs for each beach, specifically in regards to improving infrastructure and services, and obtaining certification as a sustainable beach. The evaluation of the beaches as perceived by users suggests that it would be useful to assess beach quality in order to support management goals and be applicable to other beaches, both nationally and internationally.     

Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

Based on the concentration of Malathion used in the field, we evaluated the genotoxic potential of low concentrations of this insecticide on meristematic and F₁ cells of Allium cepa and on rat hepatoma tissue culture (HTC cells). In the A. cepa, chromosomal aberrations (CAs), micronuclei (MN), and mitotic index (MI) were evaluated by exposing the cells at 1.5, 0.75, 0.37, and 0.18 mg/mL of Malathion for 24 and 48 hr of exposure and 48 hr of recovery time. The results showed that all concentrations were genotoxic to A. cepa cells. However, the analysis of the MI has showed non-relevant effects. Chromosomal bridges were the CA more frequently induced, indicating the clastogenic action of Malathion. After the recovery period, the higher concentrations continued to induce genotoxic effects, unlike the observed for the lowest concentrations tested. In HTC cells, the genotoxicity of Malathion was evaluated by the MN test and the comet assay by exposing the cells at 0.09, 0.009, and 0.0009 mg/5 mL culture medium, for 24 hr of exposure. In the comet assay, all the concentrations induced genotoxicity in the HTC cells. In the MN test, no significant induction of MN was observed. The genotoxicity induced by the low concentrations of Malathion presented in this work highlights the importance of studying the effects of low concentrations of this pesticide and demonstrates the efficiency of these two test systems for the detection of genetic damage promoted by Malathion.     

Shearwater diet during migration along the coast of Rio Grande do Sul,Brazil

The coastline of the state of Rio Grande do Sul (Brazil) is used annually as a migratory route by a number of seabird species. Thirteen surveys were carried out between July 1997 and July 1998 to obtain information on the diet of Puffinus gravis, Puffinus griseus, and Puffinus puffinus along the coast of Rio Grande do Sul. Sampling efforts focused on dead beach-cast specimens. One hundred twenty-one stomach content analyses were performed on P. gravis from a total of 238 specimens; 17 stomach content analyses were performed on P. griseus from a total of 54 specimens; and 25 stomach content analyses were performed on 25 P. puffinus from a total of 61 dead specimens. Cephalopods were the main prey found in the stomachs of the three species and were present in 96% of the P. gravis stomachs. The following cephalopods were observed: Ommastrephidae, Gonatus antarcticus, Gonatus sp., Lycoteuthis lorigera, Lycoteuthis sp., Histioteuthis sp., Cycloteuthis sp., Loligo plei, Loligo sanpaulensis, Sepiidae, and Argonauta nodosa. The latter was the most abundant species in the three species of Puffinus. The following fish were identified from otoliths: Porichthys porosissimus, Ctenosciaena gracilicirrhus, Paralonchurus brasiliensis, Cynoscion guatucupa, and Trichiurus lepturus. There was also a high incidence of synthetic materials such as plastic, polyethylene and rubber.     

Removal of a red dye using noncommercial peroxidase extracted from rice bran

The present study investigates the application of a noncommercial peroxidase enzyme, which was extracted from rice bran, to decolorize wastewater that was simulating effluent from a textile manufacturer using a red dye in its coloration process.     

Adaptive threat management framework: integrating people and turtles

In the 35 years since its inception, the Brazilian National Program for the Conservation of Marine Turtles (TAMAR) has had great success in protecting the five species of sea turtles that occur in Brazil. It has also contributed significantly to worldwide scientific data and knowledge about these species’ biology, such as life cycles and migration patterns. TAMAR’s conservation strategies have always relied on a variety of environmental education and social inclusion (EESI) activities highly adapted to the socio-environmental evolving contexts of its 25 locations distributed across nine states. Diversity and flexibility are critical to enable timely and effective local responses to existing or potential threats to sea turtles. The intuitive, locally adapted, decentralized, and independent way EESI activities have been carried out have generated positive results in the resolution of specific and evolving local problems through the course of the project. This article brings EESI under the same conceptual framework that underlies its conservation approach by adopting an adaptive threat management framework to organize and qualify its educational and social inclusion interventions according to the main categories of threat addressed by TAMAR.