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Dr. J. F. Bruch P. Metezeau N. Garcia-Fonknechten Y. Richard V. Tricottet B.-L. Hsi A. Kitzis C. Julien E. Papiernik 《黑龙江环境通报》1991,11(10):787-798
Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities. 相似文献
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Laperdina T. G. Melnikova M. V. Koval A. T. Sidorov Y. F. Nagorny V. A. Ostapchuk V. I. 《环境科学学报(英文版)》2000,(Z1)
IntroductionSiberiaandtheFarEastaretheoldestandtherichestgold miningareasofRussia .Intensivegold miningherestartedinthebeginningofthe 19thcentury(Vyazelshchikov ,1963) .Forthemostpartgold bearingoresandsandswereprocessedwiththeuseofamalgamationmethodforgolde… 相似文献
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论述了从培养目标、课程设置、教学方法与实践等方面培养高素质复合型的环境类专业的本科生,以满足社会需求,并为环境类专业本科教学改革提供参考。 相似文献
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介绍了Shell渣油造气工艺过程中的碳黑,石脑油污染,微口废水池导致硫化氮,一氧化碳,碳黑的污染及渣油泵检修带来的渣油污染,以及为保护环境消除污染而采取的技术改造措施。 相似文献
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介绍了APV喷雾干燥尾气的性质,原尾气系统存在的问题,改造中采取的措施、原理、流程和取得的效果。实践证明,用“麻石文丘里一次降温、洗涤—XP型塔板二次洗涤—旋流除雾器除雾”的改造方案,取代原“换热器降温—喷淋塔洗涤—折流脱水器脱水”的流程,取得了良好的降温、洗气和除雾效果,且工程费用低。 相似文献
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Summary Pesticide residues in soil, water, food, wildlife and other media have been analysed in several monitoring studies. The purpose was to establish the distribution of these residues across a defined area, ascertain their trend over a specific period and use the results to assess environmental compliance and safety. In the present review the availability of information on pesticide residues in various Canadian environmental components was sought. With the possible exception of localized pesticide contamination of some private water supply wells, ground and surface water, most analyses revealed concentrations below guideline limits. Only a few cases were reported of mishandling, misuse and poisoning from pesticides. Continuing surveillance programmes and in-depth and well-organized monitoring studies, with special focus on areas that are vulnerable to contamination, by both provincial and federal governments, are largely responsible for the encouraging results. This experience in the control of pesticide use and monitoring of residues in the environment should be of particular interest in developing countries.Dr A.Y. Sangodoyin is a senior lecturer in the Department of Agricultural Engineering at the University of Ibadan. He was recently involved in a study visit to the University of Alberta, Edmonton, Alberta, Canada T6G 2G7, where his co-author Dr D.W. Smith is a member of staff in the Department of Civil-Engineering. 相似文献
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