DEVELOPMENT AND TESTING OF BIOINDICATORS FOR MONITORING THE CONDITION OF ESTUARINE ECOSYSTEMS

As part of the Environmental Monitoring and Assessment Programin 1991, the field efficacy of six research indicators wasexamined in the estuarine systems of the Gulf of Mexico. Theutility of these indicators was tested by examining thoseresponses at both ends of multiple environmental gradients;namely, under minimally impacted conditions (reference) andheavily impacted conditions (by hypoxia, industrialdischarges, agricultural runoff). In addition, the geographiceffects of location (east and west of the Mississippi River)were examined. Numbers and frequencies of pathologies,abundance of splenic macrophage aggregates, and vertebralabnormalities showed response patterns that suggest widerfield testing along full gradients rather than simply theextremes of the gradient are warranted. Selected bloodproteins showed some promise but logistical constraints inestuarine environments may limit its utility. Concentrationsof selected organic compounds in bile showed no differences inthis study between references and contaminated sites.Molecular bioindicators are discussed with regard to theirpotential utility as indicators of ecosystem conditions. Ofthese potential indicators, the use of vitellogenin assays asa measure of reproductive impairment and frequency of shelldisease in selected crustaceans as an indicator ofimmunological aberrations deserve further examination.     

Comparing Environmental Conditions Using Indicators of Pollution Hazard

/ Land use/land cover classifications for 1973 and 1991, derived from the interpretation of satellite imagery, are quantified on the basis of biophysical land units in a study area in southeastern Australia. Nutrient export potentials are estimated for each land unit based on their composition of land use/land cover classes. Spatial and temporal comparisons are made of the land units based on the calculated pollution hazard indicators to provide an insight into changes in the state of the environment and the regional significance of land use changes. For example, one ecosystem, unique to the study, showed a large increase in pollution hazard over the study period as a manifestation of an 11-fold rise in cleared area and an expansion of cropping activities. The benefits to environmental management in general are discussed.KEY WORDS: Land cover change; Nutrient export; Environmental condition; Pollution hazard; Agricultural pollution; Nonpoint source pollution; Diffuse pollution; Environmental degradation     

The role of digital data entry in participatory environmental monitoring

Many argue that monitoring conducted exclusively by scientists is insufficient to address ongoing environmental challenges. One solution entails the use of mobile digital devices in participatory monitoring (PM) programs. But how digital data entry affects programs with varying levels of stakeholder participation, from nonscientists collecting field data to nonscientists administering every step of a monitoring program, remains unclear. We reviewed the successes, in terms of management interventions and sustainability, of 107 monitoring programs described in the literature (hereafter programs) and compared these with case studies from our PM experiences in Australia, Canada, Ethiopia, Ghana, Greenland, and Vietnam (hereafter cases). Our literature review showed that participatory programs were less likely to use digital devices, and 2 of our 3 more participatory cases were also slow to adopt digital data entry. Programs that were participatory and used digital devices were more likely to report management actions, which was consistent with cases in Ethiopia, Greenland, and Australia. Programs engaging volunteers were more frequently reported as ongoing, but those involving digital data entry were less often sustained when data collectors were volunteers. For the Vietnamese and Canadian cases, sustainability was undermined by a mismatch in stakeholder objectives. In the Ghanaian case, complex field protocols diminished monitoring sustainability. Innovative technologies attract interest, but the foundation of effective participatory adaptive monitoring depends more on collaboratively defined questions, objectives, conceptual models, and monitoring approaches. When this foundation is built through effective partnerships, digital data entry can enable the collection of more data of higher quality. Without this foundation, or when implemented ineffectively or unnecessarily, digital data entry can be an additional expense that distracts from core monitoring objectives and undermines project sustainability. The appropriate role of digital data entry in PM likely depends more on the context in which it is used and less on the technology itself.     

Spatiotemporal Variability of Modeled Watershed Scale Surface‐Depression Storage and Runoff for the Conterminous United States

This study explores the viability of using simulated monthly runoff as a proxy for landscape‐scale surface‐depression storage processes simulated by the United States Geological Survey’s National Hydrologic Model (NHM) infrastructure across the conterminous United States (CONUS). Two different temporal resolution model codes (daily and monthly) were run in the NHM with the same spatial discretization. Simulated values of daily surface‐depression storage (treated as a decimal fraction of maximum volume) as computed by the daily Precipitation‐Runoff Modeling System (NHM‐PRMS) and normalized runoff (0 to 1) as computed by the Monthly Water Balance Model (NHM‐MWBM) were aggregated to monthly and annual values for each hydrologic response unit (HRU) in the CONUS geospatial fabric (HRU; n = 109,951) and analyzed using Spearman’s rank correlation test. Correlations between simulated runoff and surface‐depression storage aggregated to monthly and annual values were compared to identify where which time scale had relatively higher correlation values across the CONUS. Results show Spearman’s rank values >0.75 (highly correlated) for the monthly time scale in 28,279 HRUs (53.35%) compared to the annual time scale in 41,655 HRUs (78.58%). The geographic distribution of HRUs with highly correlated monthly values show areas where surface‐depression storage features are known to be common (e.g., Prairie Pothole Region, Florida).     

Hydrocarbon Measurements During the 1992 Southern Oxidants Study Atlanta Intensive: Protocol and Quality Assurance

Abstract

A major component of the Southern Oxidants Study (SOS) 1992 Atlanta Intensive was the measurement of atmospheric nonmethane hydrocarbons. Ambient air samples were collected and analyzed by a network of strategically located automated gas chromatography (GC) systems (field systems). In addition, an extensive canister sampling network was deployed. Combined, more than 3000 chromatograms were recorded. The SOS science team targeted for quantitative analysis 56 compounds which may be substantial contributors to ozone formation or used as air mass tracers. A quality assurance program was instituted to ensure that good measurements were being made throughout the network for each target compound. Common, high-quality standards were used throughout the network. The performance of individual field systems was evaluated during the intensive through the analysis of challenge mixtures. This methodology helped to identify and correct analytical problems as they arose.     

Challenges to liberalism: The case of Australian energy policy

This paper considers liberal and nationalist economic policy approaches to the ownership and development of Australian energy resources (oil, gas, coal and uranium). In the two decades prior to 1983, Australia pursued economic policies in relation to its energy resources which could broadly be described as ‘nationalist’. Governments of the day intervened in development decisions in an effort to enhance the ‘national interest’. From 1983, along with the deregulation of the Australian economy as a whole, policy relating to energy resources was liberalised. Development of energy reserves henceforth occurred according to the dictates of the market. This paper argues that recent Australian energy policy initiatives reflect an increase in nationalist influences and a retreat from the liberalisation agenda that dominated energy policy making in the 1980s and 1990s. Three examples are discussed where policy has been influenced by a nationalist framework: (1) the domestic gas reservation policy in Western Australia; (2) Australian government efforts to promote a ‘value adding’ nuclear processing industry and (3) Australian Labor Party policy giving preferential financial incentives for gas to liquids projects. The re-emergence of nationalism in Australia is occurring either because policy makers now favour it as a path to energy security or in some cases because they believe that appeals to nationalism will generate political support.     

Spatial identification by satellite imagery of the crop–fallow rotation cycle in northern Laos

In the mountainous regions of northern Laos, shifting cultivation, or slash-and-burn agriculture, is widely practiced. However, the crop–fallow rotation cycle is becoming shorter owing to forest conservation policies and population pressure, causing loss of productivity that deleteriously affects farmers’ livelihoods in the region. To investigate regional land use conditions, we have developed a method of identifying the crop–fallow rotation cycle from Landsat Thematic Mapper (TM) and Enhanced Thematic Mapper+ (ETM+) data. We assessed the impact of the identified cycle on plant production measured by Normalized Difference Vegetation Index (NDVI). The study site was an area in Luang Prabang Province. Using eight TM and ETM+ images acquired annually from 1995 to 2003, except for 1998, when cloud-free data were not collected, we classified land use in each year as crop or fallow by the presence of vegetation in the late dry season. Conformity with fallow age determined by field investigation was 69.1%. The cultivation frequency from 1995 to 2002 showed that 77,000 ha (17.3% of the study site) had not been used for cropping during the period, but 41,000 ha (9.2%) had been used every year. Of the study site, 129,000 ha (29.1%) was cultivated one or two times, 83,000 ha (18.7%) was three or four times, and 54,000 ha (12.2%) was five or six times. The NDVI of crops in November did not provide sufficient evidence to prove the assumption that a longer fallow period would result in better crop yields. Instead, the regeneration of fallow vegetation was evidenced by the higher NDVI values after longer fallow. More than 8 years would be needed to reach the same NDVI as forest. From the produced maps indicating fallow age and cultivation frequency, we found that areas with high potential for regeneration decreased as cultivation frequency increased. Areas near rivers were intensively used, and fallow length was accordingly short. Low-potential areas were found in the western basin of the Mekong River. This spatial information can be used to detect areas where biomass productivity is at high risk of deteriorating. Readers should send their comments on this paper to: BhaskarNath@aol.com within 3 months of publication of this issue.     

Seaweed sex pheromones and their degradation products frequently suppress amphipod feeding but rarely suppress sea urchin feeding

Summary. A diverse group of brown seaweeds produce bouquets of C₁₁ metabolites, some of which act as pheromones that cue gamete release or attract sperm to eggs following release. We demonstrate that these C₁₁ metabolites and their degradation products also frequently and strongly deter feeding by the herbivorous amphipod Ampithoe longimana, but rarely by the herbivorous sea urchin Arbacia punctulata. Across the range of concentrations tested, seven of twelve C₁₁ metabolites or mixtures that we tested deterred feeding by the amphipod, but only two of eleven deterred the sea urchin. For those compounds where we could rigorously contrast the magnitude of deterrence against the amphipod with the magnitude of deterrence against the urchin, the amphipod was deterred significantly more than the urchin by five of six metabolites. Thus, C₁₁ compounds were more frequently and more strongly deterrent to the amphipod than to the sea urchin. These findings for C₁₁ metabolites conflict with previous investigations, where other classes of seaweed chemical defenses have been shown to deter feeding by large mobile herbivores like urchins and fishes but to be relatively ineffective against mesograzers, especially the species of amphipod that we used here. Our results suggest that C₁₁ metabolites are unusual among the known seaweed chemical defenses in that they are especially effective against mesograzers, which often consume seaweed spores, zygotes, and juveniles. The high concentrations of C₁₁ metabolites in brown algal eggs could allow these defenses to be especially important in defending gametes, zygotes, or young sporelings from herbivorous mesograzers. Received 26 February 1998; accepted 9 April 1998.     

Lignoid chemical defenses in the freshwater macrophyte Saururus cernuus

Summary. Chemical defense against herbivores has rarely been investigated for freshwater plants, possibly due to the common misconception that herbivory on aquatic macrophytes is low and would not select for chemical defenses. In previous work, the freshwater angiosperm Saururus cernuus was shown to be a low preference food for omnivorous crayfish despite its high nutrient value and relatively soft texture. We used feeding by the crayfish Procambarus clarkii to guide fractionation of the deterrent lipid-soluble extract of this plant, leading to the identification of seven deterrent lignoid metabolites, (–)-licarin A, (+)-saucernetin, (–)-dihydroguaiaretic acid, (–)-sauriols A and B, (–)-saucerneol, and (–)-saucerneol methyl ether. Lignans have been implicated in terrestrial plant chemical defenses as insect growth inhibitors, insect toxins, nematocides, antibacterial, and antifungal agents. However, these activities have rarely been demonstrated using ecologically relevant methodologies in terrestrial systems, and never before in freshwater systems. The widespread nature of lignans amongst very distantly related plants, along with their rich diversity of molecular structure, suggests that they could play a large role in mediating plant-herbivore interactions. In addition to the lignoid compounds we identified, there were other compounds present in low concentration or unstable compounds that were deterrent, that did not appear to be lignans, but that we were unable to identify. This plant thus appears to be defended by a complex mixture of natural products. Received 6 June 2000; revised 23 August 2000; accepted 2 September 2000