Quantification of human amniotic fluid constituents by high resolution proton nuclear magnetic resonance (NMR) spectroscopy

We have investigated the ability of high-resolution proton NMR spectroscopy to provide a biochemical constituent screening of human amniotic fluid (AF). Proton NMR spectra were obtained at 300 MHz on AF from patients undergoing amniocentesis in the mid-trimester. Only AF from normal pregnancies (normal fetal karyotype, normal a-fetoprotein levels, normal birth outcome) was used in this study. The AF supernatant was lyophilized and resuspended in deuterated water containing 0.1 mm phosphate buffer and 6.02 mm disodium maleate. Identification of low molecular weight compounds was confirmed by two-dimensional NMR spectra (primarily correlated spectroscopy, or COSY) and standard addition techniques. A broad profile of compounds were ‘NMR visible’ in a single proton spectrum, including creatinine, glucose, organic acids (acetate, citrate, and lactate) and several amino acids (alanine, histidine, leucine, phenylalanine, tyrosine and valine). The proton spectrum was unaffected by prior freezing/thawing of AF samples. We were able to quantify compounds by comparison with an added concentration standard (maleate) at concentrations as low as 30 μm. Good agreement with literature values based on other analytical techniques was obtained.     

Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal de termination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 percent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.     

Sorption-Desorption of Atrazine and Diuron in Soils from Southern Brazil

The objective of this study was to investigate the behavior of sorption and desorption of the herbicides atrazine (6-chloro-N ²-ethyl-N ⁴-isopropyl-1,3,5-triazine-2,4-diamine) and diuron [3-(3,4-dichlorophenyl)-1,1-dimethyleurea] in soil samples from a typical lithosequence located in the municipality of Mamborê (PR), southern Brazil. Five concentrations of ¹⁴C-atrazine and ¹⁴C-diuron were used for both herbicides (0.48, 0.96, 1.92, 3.84, and 7.69 mg L^?1). Sorption of both herbicides correlated positively with the organic carbon and clay content of the soil samples. Sorption isotherms were well described by the Freundlich model. The slope values of the isotherm (N) ranged from 0.84 to 0.90 (atrazine) and from 0.75 to 0.79 (diuron) for the lithosequence samples. Sorption of diuron was high regardless of the soil texture or the concentration added. The desorption isotherms for atrazine and diuron showed good fit to the Freundlich equation (R ² ≥ 0,87). Atrazine slope values for the desorption isotherms were similar for the different concentrations and were much lower than those observed for the sorption isotherms. Significant hysteresis was observed in the herbicide desorption. When the two herbicides were compared, it was found that diuron (N = 0.06–0.22) presented more pronounced hysteresis than atrazine. The results showed that, quantitatively, a greater atrazine fraction applied to these soils remains available to be leached in the soil profile, as compared to diuron.     

Integrated Assessment Modeling of Atmospheric Pollutants in the Southern Appalachian Mountains. Part I: Hourly and Seasonal Ozone

Abstract

Recently, a comprehensive air quality modeling system was developed as part of the Southern Appalachians Mountains Initiative (SAMI) with the ability to simulate meteorology, emissions, ozone, size- and composition-resolved particulate matter, and pollutant deposition fluxes. As part of SAMI, the RAMS/EMS-95/URM-1ATM modeling system was used to evaluate potential emission control strategies to reduce atmospheric pollutant levels at Class I areas located in the Southern Appalachians Mountains. This article discusses the details of the ozone model performance and the methodology that was used to scale discrete episodic pollutant levels to seasonal and annual averages. The daily mean normalized bias and error for 1-hr and 8-hr ozone were within U.S. Environment Protection Agency guidance criteria for urban-scale modeling. The model typically showed a systematic overestimation for low ozone levels and an underestimation for high levels. Because SAMI was primarily interested in simulating the growing season ozone levels in Class I areas, daily and seasonal cumulative ozone exposure, as characterized by the W126 index, were also evaluated. The daily ozone W126 performance was not as good as the hourly ozone performance; however, the seasonal ozone W126 scaled up from daily values was within 17% of the observations at two typical Class I areas of the SAMI region. The overall ozone performance of the model was deemed acceptable for the purposes of SAMI’s assessment.     

Report on International Affairs Committee

This is a report on Meetings of Representatives of National Air Pollution Preventions Associations to Consider the Establishment of an International Union, held in Washington, D. C, June 18–19, 1964 and Houston, Texas, June 21–22, 1964.     

Desulfurization of Caribbean Fuel

An American Petroleum Institute study of desulfurization of Caribbean fuel oil was completed in February 1967. The report is unique in that proprietary information was made available to the contractor, the Bechtel Corp., by task force members and licensors of desulfurization processes. Thus confidential information never before available to a single organization was used to develop feasibility and incremental costs of various processes for desulfurization. The report provides needed guideposts for decision-making by governmental units as well as by industry. Incremental costs are presented for intermediate levels of desulfurization from the normal level for Caribbean heavy fuel oil of 2.6% down to 0.5%, as well as the sensitivity of costs to sulfur credits and to rate of recovery of invested capital. Degree of justifiable confidence in the various data is discussed. Effects of desulfurization on other characterisics of the fuel oil are also discussed.     

Book reviews

URBANIZATION AND COMMUNITY BUILDING IN MODERN NORWAY

J. S. Tortenson, M. F. Metcalfe, T. F. Rasmussen

Urbana Press, Oslo 1985. 312 pp., figs and tables.

REGIONAL DEVELOPMENT POLICY

R. Riddell

Gower, 1985. £18.50

REVIEW OF SERVICE INDUSTRIES: A GEOGRAPHICAL APPRAISAL

P. W. Daniels

Methuen, 1985. £27.50.

ARABIC‐ISLAMIC CITIES: BUILDING AND PLANNING PRINCIPLES

Besim Selim Hakim

KPI Ltd., London, 1986. pp. 192. £46 (h/b)

FIFTY YEARS OF LANDSCAPE DESIGN

Sheila Harvey and Stephen Rettig

The Landscape Press, 1985. £12.50

CAPABILITY BROWN AND THE EIGHTEENTH CENTURY ENGLISH LANDSCAPE

Roger Turner

Weidenfeld & Nicholson. £16.95.     

ON THE MEASUREMENT OF ENVIRONMENTAL IMPACTS OF PUBLIC PROJECTS FROM A SOCIOLOGICAL PERSPECTIVE1

ABSTRACT: The major objectives are (1) to identify the problems involved in measuring the environmental impacts of public projects from selected perspectives, and (2) to elaborate a sociological approach used in an empirical investigation in that respect. The construct of environmental impact of a planned action is generally operationalized from different perspectives and with different methodological emphases in the various disciplines. Even the term environment does not elicit agreement among users as to its exact meaning. Although there has been a steady increase in the number of studies from a sociological perspective concerning environmental problems, there is lack of sociological counsel in writing environmental impact statements. Overall, we lack sociological methodology and operational procedures for that purpose. In an attempt to bring some empirical focus to this field, attitudinal measures employed to discover how residents of a river basin perceived negative and positive environmental impacts of a proposed watershed development project are reviewed. These come from a study of creation of the Cooper Reservoir and Dam in Texas. Data on 343 heads of households m the selected areas were collected through structured questionnaires with items on personal information, a vested interest scale, a knowledge of the project scale, and an environmental impact scale. Data show that perception of impacts by residents is influenced significantly by degree of their vested interests involved. Variables for inclusion in a sociological model of environmental impact are suggested.     

Relictual Amphibians and Old-Growth Forests

Abstract: Terrestrial and aquatic herpetofauna were sampled by pitfall traps, time-constrained searches, and area-constrained searches (stream sites only) over a three-year period to examine the importance of forest age to amphibians and reptiles. Fifty-four terrestrial and 39 aquatic sites in Douglas-fir—dominated, mixed evergreen forests were located in southwestern Oregon and northwestern California Mean age of trees on sites ranged in age from 30 to 560 years. Thirty-one species of amphibians and reptiles were detected from the 93 localities. Only three species were found primarily on older forest sites: the Del Norte salamander (Plethodon elongatus), the Olympic salamander (Rhyacotriton olympicus), and the tailed frog (Ascaphus truei). Paleoeco-logic evidence indicates an historical association between these three amphibians and the extant elements of ancient primeval coniferous forests of the Pacific Northwest. The life histories and habitat requirements of these species suggest that these forms are scarce in younger forests because the microclimatic and microhabitat conditions they require generally exist only in older forests. The long-term viability of these species in northern California and southern Oregon may depend upon developing forestry practices that protect these mitical microclimates and microhabitats.