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Previously published mtDNA cytochrome b sequences for Xyrichtys novacula (Linnaeus) and two morphologically distinct types of Xyrichtys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to
identify the two types of Xyrichtys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X.␣martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichtys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichtys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony
analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
Received: 12 March 1997 / Accepted: 24 October 1997 相似文献
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Afsheen Zehr Zulfiqar Ali Sahito Wenbin Tong Lin Tang Yasir Hami Muhammad Bilal Khan Zarina Ali Beena Naqvi Xiaoe Yang 《环境科学学报(英文版)》2020,32(1):24-38
Phytoremediation is a valuable technology for mitigating soil contamination in agricultural lands, but phytoremediation without economic revenue is unfeasible for land owners and farmers. The use of crops with high biomass and bioenergy for phytoremediation is a unique strategy to derive supplementary benefits along with remediation activities. Sunflower (Helianthus annuus L.) is a high-biomass crop that can be used for the phytoremediation of polluted lands with additional advantages (biomass and oil). In this study, 40 germplasms of sunflower were screened in field conditions for phytoremediation with the possibility for oil and meal production. The study was carried out to the physiological maturity stage. All studied germplasms mopped up substantial concentrations of Pb, with maximum amounts in shoot?>?root?>?seed respectively. The phytoextraction efficiency of the germplasm was assessed in terms of the Transfer factor (TF), Metal removal efficiency (MRE) and Metal extraction ratio (MER). Among all assessed criteria, GP.8585 was found to be most appropriate for restoring moderately Pb-contaminated soil accompanied with providing high biomass and high yield production. The Pb content in the oil of GP.8585 was below the Food safety standard of China, with 59.5% oleic acid and 32.1% linoleic acid. Moreover, amino acid analysis in meal illustrated significant differences among essential and non-essential amino acids. Glutamic acid was found in the highest percentage (22.4%), whereas cysteine in the lowest percentage (1.3%). Therefore, its efficient phytoextraction ability and good quality edible oil and meal production makes GP.8585 the most convenient sunflower germplasm for phytoremediation of moderately Pb-contaminated soil, with fringe benefits to farmers and landowners. 相似文献
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Polymerase chain reaction and direct comparison of mitochondrial DNA sequences from a cytochrome b gene fragment were used to identify two morphologically distinct larval types ofXyrichtys, a genus of tropical wrasse (Pisces: Labridae). Both larval types were collected during ichthyoplankton surveys on the Middle Atlantic Bight shelf in the summer of 1988. DNA sequence comparisons indicated that both types were larvae ofXyrichtys novacula (Linnaeus). Back-calculated birthdate distributions for those larvae collected on the Middle Atlantic Bight shelf demonstrated that the two larval types formed two distinct cohorts indicating a biological difference. The two distinct larval types may be a consequence of an ecophenotypic effect, or they may represent offspring from genetically distinct populations. These results emphasize that important biological and oceanographic information can be gained through the use of the polymerase chain reaction and DNA sequencing for larval identificaiton. 相似文献
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Ansari Madiha Zafar Urooj Ejaz Uroosa Sohail Muhammad Pirzada Aiman Aman Afsheen 《Journal of Material Cycles and Waste Management》2021,23(3):911-921
Journal of Material Cycles and Waste Management - Sugar industries produce vast quantity of sugarcane bagasse (SCB) waste which is used as raw material by biorefineries; however, the resulted... 相似文献
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Bahram Momen Larry W. Eichler Charles W. Boylen Jonathan P. Zehr 《Environmental management》1997,21(5):725-732
a (Chl a), silica (Si), and chloride (Cl) through the use of proper statistical techniques. Results indicate no statistically significant
changes in the concentrations of TP, Chl a, or Si in the spring or summer from 1981 to 1993. A significant temporal trend of increase in Cl concentration is, however,
detected. This is perhaps the strongest evidence that the development of the Lake George watershed has affected lakewater
chemistry. In spring, the concentrations of TP, Chl a, Si, and Cl, averaged over all 13 years, were higher in the south basin, but differences are not statistically significant
(i.e., P > 0.05). In summer, Si was slightly but significantly lower, and Cl was nonsignificantly higher in the south basin. Significant
interactions between temporal and spatial changes are detected based only on summer values of TP and Chl a, indicating differential trends of change for these two variables in the south and north basins during the last 13 years. 相似文献
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