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不同碳源条件下草菇内切型纤维素酶基因(eg1)转录表达的分析 总被引:1,自引:0,他引:1
利用Northernblot方法分析了不同碳源条件下草菇切型纤维素酶基因(eg1)的表达.结果发现,在含有纤维素的液体培养基中生长10d,eg1在草菇菌丝中有高效表达;纤维二糖、α-乳糖、β-乳糖,也能诱导eg1的表达,但和纤维素相比,eg1的表达量相对较低,并且它们的诱导效应在加入这类糖12h后迅速减弱;槐糖和龙胆二糖的诱导作用非常弱.在天然稻草为基质的固体栽培料生长时,草菇eg1的表达和草菇菌丝生长与出菇相对应,在菌丝生长期(d8)可见eg1的表达,d12时菌丝已长满,表达减弱,在出菇及菇体的分化及增大期,eg1的表达量逐渐增强,在成熟期达到最高水平;表明在草菇菇体发育中需要更多碳源及能源的补充,eg1在这方面起着非常重要的作用.图4参14 相似文献
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James V. Zidek Gavin Shaddick Jean Meloche Chris Chatfield Rick White 《Environmental and Ecological Statistics》2007,14(4):411-431
This paper presents a general framework for constructing a predictive distribution of the exposure to an environmental hazard
sustained by a randomly selected member of a designated population. The individual’s exposure is assumed to arise from random
movement through the environment, resulting in a distribution of exposure that can be used for environmental risk analysis.
A specialization of the general framework is that of predicting human exposure to air pollution that can be used to develop
models for such things as exposure to particulate matter; practical aspects of their construction are considered. These models
can help answer questions such as what fraction of the population sustained ‘high’ levels of exposure for say 5 days in a
row. The immediate implementation of the above framework takes the form of a computing platform referred to as pCNEM. This provides a facility for simulating exposures to airborne pollutants and is described in detail elsewhere. This paper
considers some theoretical aspects underpinning probabilistic exposure models of this type, with the ideas illustrated in
developing a model for predicting human exposure to PM
10. 相似文献
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Saulo R. Freitas Karla M. Longo Maria A. F. Silva Dias Pedro L. Silva Dias Robert Chatfield Elaine Prins Paulo Artaxo Georg A. Grell Fernando S. Recuero 《Environmental Fluid Mechanics》2005,5(1-2):135-167
The atmospheric transport of biomass burning emissions in the South American and African continents is being monitored annually using a numerical simulation of air mass motions; we use a tracer transport capability developed within RAMS (Regional Atmospheric Modeling System) coupled to an emission model. Mass conservation equations are solved for carbon monoxide (CO) and particulate material (PM2.5). Source emissions of trace gases and particles associated with biomass burning activities in tropical forest, savanna and pasture have been parameterized and introduced into the model. The sources are distributed spatially and temporally and assimilated daily using the biomass burning locations detected by remote sensing. Advection effects (at grid scale) and turbulent transport (at sub-grid scale) are provided by the RAMS parameterizations. A sub-grid transport parameterization associated with moist deep and shallow convection, not explicitly resolved by the model due to its low spatial resolution, has also been introduced. Sinks associated with the process of wet and dry removal of aerosol particles and chemical transformation of gases are parameterized and introduced in the mass conservation equation. An operational system has been implemented which produces daily 48-h numerical simulations (including 24-h forecasts) of CO and PM2.5, in addition to traditional meteorological fields. The good prediction skills of the model are demonstrated by comparisons with time series of PM2.5 measured at the surface. 相似文献
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Coatis (Nasua spp.), gregarious, omnivorous carnivores that range in forests from the southwestern USA to south America, dispatch millipedes
by rolling them on the ground using rapid, alternating movements of their forepaws. Prey rolling of millipedes is thought
to stimulate the depletion of their defensive secretions and to wipe off secretions before millipedes are consumed. We report
that prey-rolling behavior in Nasua spp. is elicited by 1,4-benzoquinone; 2-methyl-1,4-benzoquinone; and 2-methoxy-3-methyl-1,4-benzoquinone, the chief components
of the defensive secretions of julidan, spirobolidan, and spirostreptidan millipedes. Chemicals elaborated for defense sometimes
evolutionarily “backfire,” providing cues to predators on the presence or identity of prey. The elicitation of prey-rolling
behavior in Nasua spp. by benzoquinones illustrates this effect for millipedes (and possibly other arthropods) that defensively discharge these
compounds. 相似文献
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利用Northern blot方法分析了不同碳源条件下草菇內切型纤维素酶基因(eg1)的表达.结果发现,在含有纤维素的液体培养基中生长10 d,eg1在草菇菌丝中有高效表达;纤维二糖、α-乳糖、β-乳糖,也能诱导eg1的表达,但和纤维素相比,eg1的表达量相对较低,并且它们的诱导效应在加入这类糖12 h后迅速减弱;槐糖和龙胆二糖的诱导作用非常弱.在天然稻草为基质的固体栽培料生长时,草菇eg1的表达和草菇菌丝生长与出菇相对应,在菌丝生长期(d 8)可见eg1的表达,d 12时菌丝已长满,表达减弱,在出菇及菇体的分化及增大期,eg1的表达量逐渐增强,在成熟期达到最高水平;表明在草菇菇体发育中需要更多碳源及能源的补充,eg1在这方面起着非常重要的作用. 图4 参14 相似文献
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