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The new European chemical regulation (REACH) requires a short-term fish test for chemicals where the level of production exceeds 10tons per year. For ethical reasons (3R-concept), an alternative to the acute fish test should be introduced to decrease the number of animal testing with fish. The zebrafish embryo (Danio rerio) test became a valuable tool in ecotoxicology and already replaces the acute fish test for the evaluation of wastewater in Germany. Recent efforts are targeted to use this and other fish embryo tests for the effect assessment of chemicals. The toxic effects of the carbamate insecticide aldicarb and its metabolite aldicarb-sulfoxide to zebrafish embryos were analysed using two approaches with different endpoints. Organismic tests were conducted with zebrafish embryos exposed to the pesticides for 48h. In addition, suborganismic effects were examined analysing the enzyme inhibition of cholinesterases and carboxylesterases. On the organismic level, the only sublethal effect seen was the increase of heart rate at low and decrease at higher concentration with the use of aldicarb-sulfoxide but not with aldicarb (concentration range 0.2-300microM). In contrast, analysis of enzyme inhibitions showed high to very high effects caused by the two carbamates. The enzyme inhibition analysis of whole homogenates of exposed embryos may be advantageous for toxicant screening (biomarker of exposure) and might be used to bridge the gap of sensitivity of the (48h old) zebrafish embryos to adult fish when exposed to anti-cholinesterase substances (biomarker of prospective effect). 相似文献
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Cornelia Meinert Emma Schymanski Eberhard Küster Ralph Kühne Gerrit Schüürmann Werner Brack 《Environmental science and pollution research international》2010,17(4):885-897
Background, aim and scope
The importance of groundwater for human life cannot be overemphasised. Besides fulfilling essential ecological functions, it is a major source of drinking water. However, in the industrial area of Bitterfeld, it is contaminated with a multitude of harmful chemicals, including genotoxicants. Therefore, recently developed methodologies including preparative capillary gas chromatography (pcGC), MOLGEN-MS structure generation and mutagenicity prediction were applied within effect-directed analysis (EDA) to reduce sample complexity and to identify candidate mutagens in the samples. A major focus was put on the added value of these tools compared to conventional EDA combining reversed-phase liquid chromatography (RP-LC) followed by GC/MS analysis and MS library search. 相似文献7.
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Kumar V Rani S Malik S Trivedi AK Schwabl I Helm B Gwinner E 《Die Naturwissenschaften》2007,94(8):693-696
Daytime light intensity can affect the photoperiodic regulation of the reproductive cycle in birds. The actual way by which
light intensity information is transduced is, however, unknown. We postulate that transduction of the light intensity information
is mediated by changes in the pattern of melatonin secretion. This study, therefore, investigated the effects of high and
low daytime light intensities on the daily melatonin rhythm of Afro-tropical stonechats (Saxicola torquata axillaris) in which seasonal changes in daytime light intensity act as a zeitgeber of the circannual rhythms controlling annual reproduction
and molt. Stonechats were subjected to light conditions simulated as closely as possible to native conditions near the equator.
Photoperiod was held constant at 12.25 h of light and 11.75 h of darkness per day. At intervals of 2.5 to 3.5 weeks, daytime
light intensity was changed from bright (12,000 lux at one and 2,000 lux at the other perch) to dim (1,600 lux at one and
250 lux at the other perch) and back to the original bright light. Daily plasma melatonin profiles showed that they were linked
with changes in daytime light intensity: Nighttime peak and total nocturnal levels were altered when transitions between light
conditions were made, and these changes were significant when light intensity was changed from dim to bright. We suggest that
daytime light intensity could affect seasonal timing via changes in melatonin profiles.
Professor Dr. E. Gwinner died on 07 September 2004. 相似文献
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