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Particulate matter including carbon black (CB) nanoparticles can enhance antigen-related inflammation and immunoglobulin production in vivo. Dendritic cells (DC) as antigen-presenting cells (APC) are the most capable inducers of immune responses. The present study was designed to determine whether CB nanoparticles affect the maturation/activation and function of DC in vitro. DC were differentiated from bone marrow (BM) cells of BALB/c mice by culture with granulocyte macrophage colony stimulating factor (GM-CSF). At day 8 of culture, BM-derived DC (BMDC) were exposed to CB nanoparticles with a diameter of 14nm or 56nm for 24h. The expression of major histocompatibility complex (MHC) class II, DEC205, CD80, and CD86 (maturation/activation markers of BMDC) was measured by flow cytometry. BMDC function was evaluated by an allogeneic mixed lymphocyte reaction (MLR) assay. CB nanoparticles significantly increased the expression of DEC205 and CD86 in BMDC and tended to increase MHC class II and CD80 expression; however, a size-dependent effect was not observed. On the other hand, BMDC-mediated MLR was significantly enhanced by the CB nanoparticles and the enhancement was greater by 14nm CB nanoparticles than by 56nm CB nanoparticles. Taken together, CB nanoparticles can promote the maturation/activation and function of BMDC, which could be related to their effects on allergic diseases and/or responses. In addition, BMDC-mediated MLR might be useful assay for in vitro screening for adjuvant activity of environmental toxicants.  相似文献   
2.
Paper passes through many hands. In the present paper, key parts of this cycle in Japan were examined, using questionnaire surveys of households and paper makers. The study aimed to examine the paper makers' strategy for paper production and their attitude to recycling, in comparison with those of the consumers. The study especially focused on toilet paper because consumers have a lot of freedom in purchasing toilet paper. A total of 1242 consumers and 60 paper makers responded. The major findings were as follows. First, we compared the criteria of consumers for purchasing toilet paper with the conjectures of paper makers. Brand, advertisements and the appearance of shopping displays received 60% support from paper makers, but less than 12% of consumers selected these criteria even if we exclude those who do not buy toilet paper or do not have any particular criteria. On the other hand, multiple plies and benefit to the earth were selected by moderate numbers (between 20 and 25%) of consumers, while only 16.77 and 5.69%, respectively of paper makers marked these criteria. Paper makers strongly believe that many retailers sell toilet paper as a loss-leader. By comparing those matters considered important for recycling by consumers with those considered important by makers, it was shown that many makers have a clear awareness of the particular problem, the lack of used paper consumption, in the current paper recycling situation in Japan.  相似文献   
3.
This paper compares the recycling attitudes of consumers in Germany and Japan, based on our nationwide questionnaires. We analyzed consumption behavior as well as recycling behavior in narrow sense, because it is important to study the former to see how the supply of recycled materials is balanced with the demand, particularly in Japan which exports little recycled materials. Toilet paper was adopted as a specific good for study, because consumers have options of buying virgin products and recycled products. It was found that German households pay for waste collection specifically and have higher recycling rates than Japanese households. On the other hand, similar figures in German and Japanese consumers were observed with regard to consumption of recycled products. Their purchasing criteria and preference on virgin and recycled products of toilet paper were examined by self-report and blind test with 2×2 experimental design for toilet paper (German versus Japanese, virgin versus recycled). Blind test showed that people prefer domestic and virgin products in both countries. Half of respondents rating virgin products guessed that the products contained recycled material. For Japanese, the material of the sample toilet paper, made from virgin pulp, seems to be a more determining factor than the nationality of products, i.e. made in Japan. On the other hand, for Germans, the domestic nature seems to be a more dominant factor than the material, i.e. made from virgin material. Canonical discriminant analysis in conjunction with logistic regression based on self-reporting data detected the characters ‘multiplied’, ‘appearance’ and ‘brand’ as major factors making the difference in preference between the two countries. The possible reasons causing the similarity and dissimilarity between the two countries are discussed with reference to the background history.  相似文献   
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Food and Environmental Virology - Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The “micro-amount of virion enrichment...  相似文献   
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We have been continuously observing the daily 7Be concentrations in surface air at Yamagata, Japan (38.25° N, 140.35° E) since 2000. The yearly profile of the 7Be concentration indicates the variation in galactic cosmic rays owing to solar modulation. Over 8 y, the 7Be concentration, cosmic neutrons, and number of sunspots varied by 37.4%, 12.2%, and 92.8%, respectively. The influence of precipitation on the 7Be variability was approximately 5%. Hence, the yearly 7Be concentration was mainly varied by the solar modulation of the 7Be production rates. Based on the production rates found in an EXPACS simulation, the observed variability indicates 7Be transport from high latitudes. The daily 7Be concentrations have two significant periodic components of 19 d and 36 d. The 36-d component implies a relationship between the sun's rotation and the vertical transport of air masses under quiet solar activity.  相似文献   
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