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排序方式: 共有79条查询结果,搜索用时 156 毫秒
1.
Microbial succession associated with organic matter decomposition during thermophilic composting of organic waste. 总被引:3,自引:0,他引:3
Using dog food as a model of the organic waste, thermophilic composting was carried out for 14 days at a fixed temperature of 60 degrees C. The relationship between organic matter decomposition measured by CO2 evolution during the bio-stabilization process and microbial succession expressed as the changes over time in the restriction fragment length polymorphism (RFLP) patterns of 16S rDNA sequences, of micro-organisms associated with the composting material was also examined. The CO2 evolution rate peaked on day 3 and gradually decreased until it became extremely small after day 9 of composting, indicating that vigorous organic matter decomposition ceased around this time. On the other hand, the RFLP pattern changed drastically from day 0 to day 4 or 5, then remained stable until day 7 or 8, reaching its final configuration, with little variations, after day 9 of composting. RFLP analysis therefore indicates that microbial succession continued into the later stage of composting. Nevertheless, by day 9, the rate of organic matter decomposition was so low that its influence on microbial populations could be hardly recognized by conventional methods of dilution plating. Moreover, the compost produced by day 9 showed no inhibitory effect on the growth of Komatsuna (Brassica campestris L. var. rapiferafroug), indicating that the maturity of compost is sufficient for plant growth when the rate of organic matter decomposition has become extremely low and the RFLP patterns become stable. 相似文献
2.
Effects of humic acids derived from lignite and cattle manure on antioxidant enzymatic activities of barley root 总被引:1,自引:0,他引:1
Kenya Nagasawa Binhui Wang Kazuki Nishiya Kensuke Ushijima Qianqian Zhu 《Journal of environmental science and health. Part. B》2016,51(2):81-89
To investigate the effects of humic acids (HAs) on the ability of plants to defend themselves against oxidative stress, barley was hydroponically cultured in the absence and presence of HAs, and the antioxidant enzymatic activities (catalase, superoxide dismutase, ascorbate and glutathione peroxidases) of root tissue were evaluated. Auxin-like structures in HAs, which were extracted from an oxidation product of lignite (LHA) and compost derived from cattle manure (CHA), were identified by pyrolysis-gas chromatography/mass spectrometry (GC/MS) with tetramethylammonium hydroxide. The LHA, which had the lower molecular weight, was more effective in promoting the growth of barley root than CHA. However, the amounts of auxin-like structures in the CHA were much higher than those for LHA. The antioxidant enzymatic activities were initially decreased in the presence of LHA and CHA at the first day after refreshing the culture solution, but were significantly increased on the second day. The CHA sample, which contained relatively high levels of phenolic acids that contained auxin-like structures, was effective in increasing four types of enzymatic activities, while the activities of catalase and ascorbate peroxidase were increased in the presence of LHA, which contains naphthalene derivatives. These results indicate that using HAs as a supplement can be effective in enhancing antioxidation enzymatic activities, while the appearance of the effects is retarded because of the decomposition and release of auxin-like compounds from HAs by organic acids from the plant roots. 相似文献
3.
We need to ensure the sustainable management of advanced materials, such as purified silicon, that contribute to a low-carbon
society. Because a drastic increase in the demand for photovoltaic (PV) systems is tightening the supply of silicon for PV
cells, the sustainability of silicon feedstock needs to be explored. For this purpose, a material flow analysis of silicon
in Japan from 1996 to 2006 is presented in this paper. Our analysis finds that rapid growth in demand for polycrystalline
silicon (pc-silicon) and single crystalline silicon (sc-silicon) has changed the structure of the purified silicon supply.
The strong demand for purified silicon for solar cells is responsible for this change. While off-grade silicon obtained as
a by-product of electronic-grade silicon (EG-Si) covered the demand for solar sells before 2000, pc-silicon is currently produced
independently for solar cells via an energy-intensive process. Analysis of the resource effective-use index (REI), which indicates
how effectively purified silicon is used, shows progress in the effective use of pc- and sc-silicon. REI analysis indicates
that the effective use of pc-silicon is reaching a maximum, while the effective use of sc-silicon is advancing, with a corresponding
increase in price. To ensure a sustainable supply of silicon feedstock, this paper proposes four solutions: (1) production
of solar-grade pc-silicon by a less costly and less energy-consuming method; (2) reduction in the amount of crystalline silicon
per watt in solar cells; (3) acceleration of the development and deployment of other solar cell types; and (4) reuse and recycling
of solar cells in the future. 相似文献
4.
Mohd Yusoff Nurulnadia Jiro Koyama Seiichi Uno Asami Kito Emiko Kokushi Eugene Tan Bacolod Kazuki Ito Yasutaka Chuman 《Environmental monitoring and assessment》2014,186(3):1453-1463
This study presents the levels of endocrine disrupting chemicals (EDCs) accumulated by Paraprionospio sp. from the Yodo River mouth, Osaka Bay. Since high concentrations of nonylphenol (NP), bisphenol A (BP), octylphenol (OP), 17β-estradiol (E2), and estrone (E1) have been measured in sediment from Osaka Bay, some bioaccumulation could be expected particularly in benthic animals. EDCs were analysed in Paraprionospio sp., a dominant benthic species in Osaka Bay. The results showed that Paraprionospio sp. had accumulated varying concentrations (wet weight; w.w.) of NP at 1,460–4,410 ng/g; BP at 22.5–39.6 ng/g; OP at 18.9–45.4 ng/g; E2 at 0.89–4.35 ng/g; and E1 at 0.06–2.50 ng/g. Accumulation of NP and OP were highest among the samples gathered in summer (July 2008), while concentrations of BP, E2, and E1 did not much differs within 3 years. EDC levels in Paraprionospio sp. were apparently greater than those in sediments showing bioaccumulation. 相似文献
5.
Effects of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and coplanar polychlorinated biphenyls (Co-PCBs) on thyroid hormone and immune response systems were examined in 16 Yusho patients at about 30 years after the outbreak of the Yusho accident. Their toxic equivalent (TEQ) levels in the blood were 27.8-1048.5 pg/g fat with the median level of 222.4 pg/g fat, which was about seven times higher than that of healthy Japanese people. Even at such high blood TEQ concentrations, they seemed not to affect the serum levels of thyroid hormones, thyroid stimulating hormone (TSH), immunoglobulins (A, G and M), autoantibodies (antinuclear antibody, rheumatoid and lupus erythematosus (LE) factors), and lymphocyte subsets in the blood. However, positive rates of rheumatoid factor were considered to increase in higher blood TEQ groups. This investigation was done using rather small number of Yusho patients, so further large-scale investigations are needed to get more conclusive findings concerning their effects on thyroid hormone and immune response systems. 相似文献
6.
Naoya Tachibana Kenya Nagasawa Binhui Wang Kazuki Nishiya Masami Fukushima Hikari Kanno Takuro Shinano Keiki Okazaki 《Chemosphere》2013
The effects of water-extractable organic matter (WEOM) from compost-like materials on peroxidative stress were investigated for hydroponic culture of barley exposed to Cd. In the presence of WEOM, lipoxygenase activity and malondialdehyde, indices of peroxidative stress in barley, were significantly reduced, compared to those with Cd alone (5 μM) for a 30-d culture (p < 0.05). In addition, Cd uptake in the presence of WEOM samples was significantly lower than that in their absence (p < 0.05). These results indicate that the addition of WEOM can be effective in mitigating the peroxidative stress in barley exposed to Cd. Of the total Cd in the solution, 7–8% was complexed with WEOM, indicating that the complexation of Cd with WEOM is a minor factor in reducing Cd-induced stress in barley. The WEOM sample was purified by cation-exchange column and ultrafiltration to remove the nutrient minerals, such as Ca, Mg and Fe. When the purified WEOM was employed for hydroponic culture in the presence of Cd, significant decreases in peroxidative stress and Cd uptake were observed (p < 0.05). These results show that the organic components in WEOM contribute to the mitigation of peroxidative stress in barley exposed to Cd. 相似文献
7.
A regional climate change impact assessment was conducted which investigated the timing of the first appearance of furunculosis
caused by the bacterium, Aeromonas
salmonicida, in fish of the Ouje-Bougoumou region of northern Quebec, Canada. Historical data were used to assess whether there was a
temporal relationship between increased temperatures (observed climate data) and the appearance of furunculosis (traditional
environmental knowledge was used to provide context). To project future impacts of climate change, climate models and lake
models were used. Regression analysis revealed a significant, positive temporal trend in mean air temperature. The temperature
range conducive to A. salmoncida survival coincided with the first appearance of furunculosis. In addition, it is predicted that lake temperatures will remain
suitable for the presence of A. salmonicida into the future; it is likely that the disease will persist throughout the twenty-first century. Climate change appears to
be a factor explaining the onset of furunculosis; however, other factors/stressors cannot be discounted, such as, the effects
past mining activities near the lakes of the Ouje-Bougoumou region have had on the body burden of contaminants in the fish
(and the potential effects on the fish immune system). 相似文献
8.
Policing behavior that prevents workers from laying male eggs was examined in the monogynous and monandrous ponerine ant
Diacamma sp. from Japan, in which a singly mated worker called a “gamergate” reproduces as the functional queen in each colony. Since
oviposition by virgin workers is rare in the presence of a gamergate, we separated a portion of workers from the gamergates
and induced their oviposition experimentally. When orphaned workers had started to oviposit, they were returned to the original
colonies, where they continued to lay eggs for a while. The gamergates and other workers interfered with the laying workers
by aggressively taking and finally eating the eggs. In total, 60% and 29% of the worker-derived eggs were eaten by gamergates
and non-mother workers, respectively. The observed worker-worker interactions were not driven simply by competition to leave
own sons, because non-laying non-orphaned workers interfered with worker reproduction. Furthermore, orphaned workers were
usually attacked by non-orphaned workers soon after colony reunification. These results indicate that both queen policing
by gamergates and worker policing in this species are mechanisms inhibiting worker oviposition. The gamergate contribution
to policing was proportionately larger than that of workers, but among virgin workers, the relationship between dominance
rank and contribution to policing was not clear. But about 11% of the eggs were not policed and were added to egg piles, especially
in large colonies. Worker policing in a monandrous and monogynous eusocial Hymenoptera contrasts to other recent findings,
and possible genetic, social, and ecological factors for its evolution in Diacamma sp. are discussed.
Received: 16 November 1998 / Received in revised form: 9 February 1999 / Accepted: 21 February 1999 相似文献
9.
Obligate parthenogenesis and reproductive division of labor in the Japanese queenless ant Pristomyrmex pungens 总被引:4,自引:0,他引:4
K. Tsuji 《Behavioral ecology and sociobiology》1988,23(4):247-255
Summary Two types of workers were recognized in colonies of Pristomyrmex pungens: extranidal workers (which characteristically walk outside the nest) and intranidal workers (which characteristically stay inside the nest). The ovaries of extranidal workers showed little activity, whereas those of intranidal workers showed high activity and often contained mature oocytes. I therefore conclude that only the intranidal workers reproduce. A behavioral repertoire of 103 individuals was obtained and used to infer group subdivision using cluster analysis; in addition, principal component analysis was performed on the intranidal workers in this set. These data enabled objective separation of extranidal and intranidal workers. Intranidal workers were larger in size on average than extranidal workers; however, the distributions overlapped. Three tests for further subdivision within the group of intranidal workers indicated that such subdivision is weak, and it is also likely that all intranidal workers lay eggs. There was no significant correlation between body size and reproductive status. The number of mature oocytes per ant fitted a Poisson distribution, and the first two principal component factors scores of behavior showed significant correlation with head width. All extranidal workers had resorbed ovaries and also had yellow bodies (which indicated a history of oviposition). When and how the differentiation between the reproductive intranidal workers and the non-reproductive extranidal workers occurred is discussed. The best-supported hypothesis is that extranidal workers are old intranidal ones. Neither males nor inseminated workers were found in any smaples collected in the field or studied in the laboratory, which greatly strengthens earlier suggestions that Pristomyrmex pungens is the first-known ant to be obligately thelytokous. These findings indicate that Pristomyrmex pungens is no longer eusocial, although it has the highest form of social behavior of any thelytokous species; they also raise the question of whether or not there are factors promoting the loss of eusociality and sexuality in this species. Ecological factors are tentatively indicated, namely, the need to maintain large colonies in the face of a nomadic lifestyle involving frequent colony fragmentation. 相似文献
10.
To develop a method for the determination of photosynthetic pigment species in individual phytoplankton cells, especially natural picophytoplankton cells, the fluorescence spectra of intact cells were studied with cultured phytoplankton. The study was made mainly with phycoerythrin-containing picophytoplankton collected off Japan in 1982 with reference to diatomal species and phycoerythrin-free cyanophycean species. The spectra were measured for cell suspensions with an ordinary spectrofluorometer, and for individual cells with a microscope spectrofluorometer, paying special attention to the effect of cell-fixation. Results indicated that: (1) the cell-fixation with the glutaraldehyde and paraformaldehyde mixture modified phycoerythrin emission from picophytoplankton markedly in its wavelength location and intensity, but (2) the emission from phycocyanin was affected far less, and (3) the emission from chlorophyll a was not altered. However, the phycoerythrin emission modified by the fixation was found to be easily distinguished from other emissions, and kept its intensity high enough for detection with a fluorescence microscope. The fluorescence properties after the fixation were kept unaltered for a long period of time. Based on the results, we propose a simple method for the determination of photosynthetic pigments in individual phytoplankton cells in seas and lakes using fluorescence microscopy. Results of our tests with natural samples of phytoplankton are presented, and problems for further improvement are also discussed. 相似文献