排序方式: 共有3条查询结果,搜索用时 15 毫秒
1
1.
Christine Susan Fagnant Alexandra Lynn Kossik Nicolette Angela Zhou Liliana Sánchez-Gonzalez Jill Christin Falman Erika Karen Keim Yarrow Linden Alana Scheibe Kilala Sayisha Barnes Nicola Koren Beck David S. Boyle John Scott Meschke 《Food and environmental virology》2017,9(4):383-394
Environmental surveillance of poliovirus (PV) and other non-enveloped viruses can help identify silent circulation and is necessary to certify eradication. The bag-mediated filtration system is an efficient method to filter large volumes of environmental waters at field sites for monitoring the presence of viruses. As filters may require long transit times to off-site laboratories for processing, viral inactivation or overgrowth of bacteria and fungi can interfere with virus detection and quantification (Miki and Jacquet in Aquatic Microb Ecol 51(2):195–208, 2008). To evaluate virus survival over time on ViroCap? filters, the filters were seeded with PV type 1 (PV1) and/or MS2 and then dosed with preservatives or antibiotics prior to storage and elution. These filters were stored at various temperatures and time periods, and then eluted for PV1 and MS2 recovery quantification. Filters dosed with the preservative combination of 2% sodium benzoate and 0.2% calcium propionate had increased virus survival over time when stored at 25 °C, compared to samples stored at 25 °C with no preservatives. While elution within 24 h of filtration is recommended, if storage or shipping is required then this preservative mixture can help preserve sample integrity. Addition of an antibiotic cocktail containing cephapirin, gentamicin, and Proclin? 300 increased recovery after storage at 4 and 25 °C, when compared to storage with no antibiotics. The antibiotic cocktail can aid sample preservation if access to appropriate antibiotics storage is available and sample cold chain is unreliable. This study demonstrated that the use of preservatives or antibiotics is a simple, cost-effective method to improve virus detection from ViroCap cartridge filters over time. 相似文献
2.
Christine Susan Fagnant Liliana Margarita Sánchez-Gonzalez Nicolette A. Zhou Jill Christin Falman Michael Eisenstein Dylan Guelig Byron Ockerman Yifei Guan Alexandra Lynn Kossik Yarrow S. Linden Nicola Koren Beck Robyn Wilmouth Evans Komen Benlick Mwangi James Nyangao Jeffry H. Shirai Igor Novosselov Peter Borus David S. Boyle John Scott Meschke 《Food and environmental virology》2018,10(1):72-82
Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Health Organization (WHO). In this study, the BMFS design was improved and tested for its usability in wastewater and wastewater-impacted surface waters in Nairobi, Kenya. Modifications made to the BMFS included the size, color, and shape of the collection bags, the filter housing used, and the device used to elute the samples from the filters. The modified BMFS concentrated 3–10 L down to 10 mL, which resulted in an effective volume assayed (900–3000 mL) that was 6–20 times greater than the effective volume assayed for samples processed by the WHO algorithm (150 mL). The system developed allows for sampling and in-field virus concentration, followed by transportation of the filter for further analysis with simpler logistics than the current methods. This may ultimately reduce the likelihood of false-negative samples by increasing the effective volume assayed compared to samples processed by the WHO algorithm, making the BMFS a valuable sampling system for wastewater and wastewater-impacted surface waters. 相似文献
3.
Zhou Nicolette A. Fagnant-Sperati Christine S. Komen Evans Mwangi Benlick Mukubi Johnstone Nyangao James Hassan Joanne Chepkurui Agnes Maina Caroline van Zyl Walda B. Matsapola Peter N. Wolfaardt Marianne Ngwana Fhatuwani B. Jeffries-Miles Stacey Coulliette-Salmond Angela Peñaranda Silvia Shirai Jeffry H. Kossik Alexandra L. Beck Nicola K. Wilmouth Robyn Boyle David S. Burns Cara C. Taylor Maureen B. Borus Peter Meschke John Scott 《Food and environmental virology》2020,12(1):35-47
1