Nutritional risks of vegan diets to women and children: Are they preventable?

The potential health risks of vegan diets specifically for women and children are discussed. Women and children are at higher risk of malnutrition from consumption of unsupplemented vegan diets than are adult males. Those who are very young, pregnant, lactating, elderly, or who suffer from poverty, disease or other environmentally induced disadvantages are at special risk. The size of these risks is difficult to quantify from existing studies. Fortunately the risk of dietary deficiency disease can be avoided and the potential health benefits of vegan diets can be realized when diets for these groups are planned in line with theRecommended Dietary Allowances so that nutrient intakes reach or exceed recommended levels, and access to preventive and curative health services is assured.     

Photodynamic action of some sensitizers by photooxidation of luminol

We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO₂ detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected.