Macroinvertebrate communities in agriculturally impacted southern Illinois streams: patterns with riparian vegetation, water quality, and in-stream habitat quality

Relationships between riparian land cover, in-stream habitat, water chemistry, and macroinvertebrates were examined in headwater streams draining an agricultural region of Illinois. Macroinvertebrates and organic matter were collected monthly for one year from three intensively monitored streams with a gradient of riparian forest cover (6, 22, and 31% of riparian area). Bioassessments and physical habitat analyses were also performed in these three streams and 12 other nearby headwater streams. The intensively monitored site with the least riparian forest cover had significantly greater percent silt substrates than the sites with medium and high forest cover, and significantly higher very fine organics in substrates than the medium and high forested sites. Macroinvertebrates were abundant in all streams, but communities reflected degraded conditions; noninsect groups, mostly oligochaetes and copepods, dominated density and oligochaetes and mollusks, mostly Sphaerium and Physella, dominated biomass. Of insects, dipterans, mostly Chironomidae, dominated density and dipterans and coleopterans were important contributors to biomass. Collector-gatherers dominated functional structure in all three intensively monitored sites, indicating that functional structure metrics may not be appropriate for assessing these systems. The intensively monitored site with lowest riparian forest cover had significantly greater macroinvertebrate density and biomass, but lowest insect density and biomass. Density and biomass of active collector-filterers (mostly Sphaerium) decreased with increasing riparian forest. Hilsenhoff scores from all 15 sites were significantly correlated with in-stream habitat scores, percent riparian forest, and orthophosphate concentrations, and multiple regression indicated that in-stream habitat was the primary factor influencing biotic integrity. Our results show that these "drainage ditches" harbor abundant macroinvertebrates that are typical of degraded conditions, but that they can reflect gradients of conditions in and around these streams.     

Acoustic underwater signals with a probable function during competitive feeding in a tadpole

Acoustic communication is widespread among adult stages of terrestrial animals and fish and has also been observed in insect larvae. We report underwater acoustic communication in the larvae of a frog, Gephyromantis azzurrae, from Isalo, a sandstone massif in western Madagascar. According to our field data, these tadpoles live in streams and prefer habitats characterized by comparatively low temperatures, shallow water depth, and a relatively fast current. Feeding experiments indicated that the tadpoles are carnivorous and macrophagous. They consumed insect larvae and, to a lesser extent, small shrimps, and conspecific as well as heterospecific tadpoles. Calls of these tadpoles consisted either of single click notes or of irregular series of various clicks. Some complex calls have a pulsed structure with three to nine indistinct energy pulses. Production of the pulses coincided with rapid closure of the jaw sheaths and often with an upward movement of the body. Calls were emitted while attacking prey and occurred significantly more often when attacking conspecifics. Tadpoles that had not been fed for some time emitted sounds more frequently than those that had been regularly fed. The spectral frequency of the calls differed in tadpole groups of different size and was higher in groups of smaller tadpoles, suggesting that spectral frequency carries some information about tadpole size which might be important during competitive feeding to assess size and strength of competitors. This report differs from those for the larvae of South American horned frogs, Ceratophrys ornata. These are the only other tadpoles for which sound production has reliably been reported but the calls of Ceratophrys tadpoles occur mainly in a defensive context.     

Aggression and resource sharing among foundresses in the social wasp Polistes dominulus: testing transactional theories of conflict

Tug-of-war models of within-group conflict predict that the frequency of conflict will be positively related to the degree of reproductive sharing within the group; in contrast, a negative relationship supports transactional models, in which reproductive payments among group members limit the degree of within-group selfishness. We tested predictions of the tug-of-war and transactional models by examining cofoundress interactions during the founding (preworker) phase of colony development in 30 naturally nesting colonies of the paper wasp Polistes dominulus. We found that the mean rate of foundress aggression and the mean probability of food sharing were significantly negatively associated, which supports the prediction of the transactional, not the tug-of-war model. Further, cofoundress aggression significantly increased over the founding phase (independently of temperature), while the fraction of aggression initiated by the dominant (alpha) foundress significantly decreased over this period. We show that both of these results are predicted by the transactional model of within-group conflict. Interestingly, the alpha’s rate of aggression was significantly positively temperature dependent, while the beta’s was not. This indicates that the alpha’s aggression level may often be near her physiological maximum, while the beta’s aggression is limi- ted by other factors, contradicting the prediction of the tug-of-war model. Moreover, the alpha’s aggression was significantly positively temperature dependent only in the second half of the founding period, as predicted by the transactional model since this is when there is least reproductive sharing. Finally, our results indicate that the alpha’s level of aggression depends on the resources controlled by the beta. Received: 18 January 2000 / Revised: 19 June 2000 / Accepted: 24 June 2000     

A comparative evaluation of accelerated solvent extraction and Polytron extraction for quantification of lipids and extractable organochlorine in fish

Optimal conditions for extraction methods using an accelerated solvent extractor (ASE) and a Polytron homogenizer were established for quantification of (crude) lipids and extractable organochlorine (EOCl) in fish. The two methods, an ASE consecutive extraction at 55 and 100 degrees C for ground, freeze-dried filets, and a Polytron extraction at room temperature with four repetitions for ground, wet filets and with two repetitions for wet fish livers were evaluated in terms of reproducibility and comparability. With respect to lipid measurement, the two methods have comparable reproducibility and the experimental errors are relatively small compared to natural variations of fish to fish within a sampling site. For EOCl measurement, the relative standard deviation tends to increase with decreasing levels of EOCl in specimens. An important factor for the relative standard deviation of measurements of analytes present at trace levels in fish matrices is sampling error in taking aliquots. Statistical analysis using a paired comparison design showed that measurement values (crude lipids or EOCl) obtained from these two extraction methods are, in general, not equivalent. There is a good correlation between the two methods for lipid measurement; whereas for EOCl measurements, high degrees of correlation exist within the exposed fish group, but not in the reference group.     

Direct on-filter assay of subtilisin-type proteolytic enzymes for rapid analysis of analyte captured from the workplace atmosphere

A simple assay for some proteolytic enzymes has been developed which can be performed directly on the surface of a cellulose nitrate filter used to capture the analyte during workplace monitoring for health and safety purposes. Following air sampling the analysis is performed on the filter which is retained within the air sampler. This involves two steps: first, a 15 min incubation in which the captured enzyme is dissolved and then digests an alkaline-phosphatase-labelled antibody immobilised as a small dot on the surface of the filter; and second, is a 10 min incubation with substrate solution, which follows an in situ wash under a vacuum. During the incubation colour develops on the spot at the location of the immobilised enzyme antibody conjugate. The intensity of the spot can be assessed visually within the sampler to ascertain the presence or absence of captured enzyme, or alternatively quantitative results can be obtained using an optical scanner. The limit of detection is 5 ng per filter for subtilisin (20 ng for visual discrimination between this standard and the zero). The assay is stable to the effects of ambient air sampling at 31 min(-1) for 18 h.     

Two experimental tests of the relationship between group stability and aggressive conflict in Polistes wasps

Two field experiments were used to examine how the relative benefits of cooperation influence within-group conflict in foundress associations of the paper wasp Polistes dominulus. P. dominulus foundresses can either nest alone or cooperate with other foundresses. We experimentally manipulated the relative benefits of co-foundress associations vs independent reproduction and tested the effect on aggressive within-group conflict. First, we examined aggression between alpha and beta co-foundresses before and after lower-ranking foundresses were removed. Removal of subordinates increases the relative contributions of the remaining subordinates to group reproductive output as there are fewer adults to care for the brood. Transactional models predict that group conflict over reproductive shares will increase as the relative benefits of grouping increase. As predicted, aggression between the co-foundresses significantly increased following subordinate removal. Second, we experimentally reduced ecological constraints on independent nesting by placing a previously orphaned, adoptable nest comb near the occupied nests. Providing an independent breeding opportunity is predicted to increase the benefits of independent reproduction relative to those of cooperating, thereby reducing group stability and aggression. As predicted, aggression between dominant and subordinate foundresses significantly decreased after the orphaned comb was presented. Therefore, group members sense variation in ecological constraints and relative productivity contributions and quickly modulate their behavior in response. Overall, these two experiments suggest that paper wasps behave as if within-group competition is limited by the threat of group dissolution such that stable groups where cooperation is strongly favored can withstand higher levels of conflict than unstable groups.     

Laboratory culture of the lobate ctenophore Mnemiopsis mccradyi with notes on feeding and fecundity

Collection and culture of the large lobate ctenophore Mnemiopsis mccradyi Mayer is described, including the requirements for successful development of larvae. Particular attention must be given to the collection of these delicate animals, the handling and provision of live microzooplankton of suitable size for the larvae, and the provision of food densities for the adults which neither stimulate “wasteful” feeding nor limit their growth. Although these ctenophores will ingest detritus and algal cells in high concentration, they lost weight at the same rate as starved individuals unless provided with living zooplankton. Under optimum conditions, specimens would lay eggs within 13 days of their own birth. By the 17th day they laid eggs daily, and had produced an average of 8,000 eggs within 23 days after birth. The maximum number of eggs laid by a single wild individual within 24 h after being brought into the laboratory was 10,000. Their high fecundity, rapid generation time, and ability to self-fertilize help to explain their sudden appearance in bloom proportions at periods of high food concentration in the environment, often referred to in the literature.     

The Role of Headwater Wetlands in Altering Streamflow and Chemistry in a Maine,USA Catchment1

Morley, Terry R., Andrew S. Reeve, and Aram J.K. Calhoun, 2011. The Role of Headwater Wetlands in Altering Streamflow and Chemistry in a Maine, USA Catchment. Journal of the American Water Resources Association (JAWRA) 1‐13. DOI: 10.1111/j.1752‐1688.2011.00519.x Abstract: Headwater wetlands, including hillside seeps, may contribute to downstream systems disproportionately to their relatively small size. We quantified the hydrology and chemistry of headwater wetlands in a central Maine, USA, catchment from 2003 to 2005 to determine their role in maintaining headwater streamflow and in affecting stream chemistry. A few of these headwater wetlands, commonly referred to as “seeps,” were characterized by relatively high groundwater discharge. During summer base flow, seeps were the primary source of surface water to the stream, contributing between 40 and 80% of stream water. Comparisons of groundwater and surface water dominant ion chemistry revealed only slight differences at the bedrock interface; however, significant changes occurred at the shallow groundwater‐surface water interface where we found decreases in total and individual cation concentrations with decreasing depth. Seep outflows significantly increased total cation and calcium concentrations in streams. Outflows at two seeps produced relatively high nitrate concentrations (88 ± 15 and 93 ± 15 μg/l respectively), yet did not correspond to higher nitrate in stream water below seep outflows (2 ± 1 μg/l). We demonstrate that small wetlands (< 1,335 m²) can contribute to headwater stream processes by linking groundwater and surface‐water systems, increasing the duration and magnitude of stream discharge, and by affecting stream chemistry, particularly during periods of base flow.