Development of indirect competitive ELISA using egg yolk-derived immunoglobulin (IgY) for the detection of Gentamicin residues

Gentamicin (Gent) is an aminoglycoside antibiotic being used in livestock sector. Gent residues could cause some genetic disorders by nonsense mutations. This study aimed to develop IgY-based ELISA for the detection of Gent in animal products. Gent was conjugated with Bovine serum albumin (BSA) by carbodiimide method for further immunization in the laying chickens. PEG-6000 extraction method was employed to extract IgY from the egg yolk. The titer of anti-Gent-IgY attained the peak of 1:256,000 after the 5^th booster immunization. Checkerboard titration confirmed that, anti-Gent IgY in 1:2,000 dilution could give an Optical Density (OD) 1.0 at 2 µg mL^?1 of Gent-OVA coating concentration. IgY-based indirect competitive ELISA (Ic-ELISA) showed that, the IC₅₀ value of anti-Gent IgY was 2.69 ng mL^?1 and regression curve equation was y = ?16.27x + 56.97 (R² = 0.95, n = 3), confirming that, the detection limit (LOD, IC₁₀ value) was 0.01 ng mL^?1. Recoveries from fresh milk, pork and chicken samples were ranged from 69.82% to 94.32%, with relative standard deviation lower than 10.88%. Our results suggested that generated anti-Gent IgY antibodies can be used in routine screening analysis of Gent residues in food samples.     

Simulating the effects of urbanization,afforestation and cropland abandonment on a regional carbon balance: a case study for Central Germany

The newly developed model system HILLS is used to simulate recent (1990–2000) and future (up to 2020) changes in land use and carbon sequestration over Central Germany. HILLS is unique as that it integrates the spatially explicit land-use-change model LUC-Hesse with the dynamic ecosystem model Century under a GIS platform. With this new tool, the concurrent effects of urbanization, afforestation and cropland abandonment on regional carbon sequestration are analyzed for an exemplary “Business as Usual” scenario. During the simulation period, afforestation was estimated to sequester 880 Gg C and cropland abandonment 783 Gg C. Urbanization was estimated to release 336 Gg C formerly stored in soil organic matter and thereby offsets about 20% of the C sequestered by cropland abandonment and afforestation. The case study shows that urbanization can partly counteract the benefits of carbon sequestration resulting from other land-use changes and should be investigated in other carbon balances.