Investigation of an Escherichia coli environmental benchmark for waterborne pathogens in agricultural watersheds in Canada

Canada's National Agri-Environmental Standards Initiative sought to develop an environmental benchmark for low-level waterborne pathogen occurrence in agricultural watersheds. A field study collected 902 water samples from 27 sites in four intensive agricultural watersheds across Canada from 2005 to 2007. Four of the sites were selected as reference sites away from livestock and human fecal pollution sources in each watershed. Water samples were analyzed for Campylobacter spp., Salmonella spp., Escherichia coli O157:H7, Cryptosporidium spp., Giardia spp., and the water quality indicator E. coli. The annual mean number of pathogen species was higher at agricultural sites (1.54 ± 0.07 species per water sample) than at reference sites (0.75 ± 0.14 species per water sample). The annual mean concentration of E. coli was also higher at agricultural sites (491 ± 96 colony-forming units [cfu] 100 mL(-1)) than at reference sites (53 ± 18 cfu 100 mL(-1)). The feasibility of adopting existing E. coli water quality guideline values as an environmental benchmark was assessed, but waterborne pathogens were detected at agricultural sites in 80% of water samples with low E. coli concentrations (<100 cfu 100 mL(-1)). Instead, an approach was developed based on using the natural background occurrence of pathogens at reference sites in agricultural watersheds to derive provisional environmental benchmarks for pathogens at agricultural sites. The environmental benchmarks that were derived were found to represent E. coli values lower than geometric mean values typically found in recreational water quality guidelines. Additional research is needed to investigate environmental benchmarks for waterborne pathogens within the context of the "One World, One Health" perspective for protecting human, domestic animal, and wildlife health.     

Der Einfluß des Magnesiums auf den Einbau von C14-Na-acetat in Fette und Lipoide junger Ratten

The Science of Nature -     

Asbestos fibres introduce trace metals into streamwater and sediments

Based on a case study in the Sumas River, it is demonstrated that asbestos fibres, which were introduced by a massive landslide, have altered the water quality and sediment conditions in the downstream section of the river. Asbestos fibres, because of their small size, are readily transported and resuspended i in stream systems. Associated with the fibres are high quantities of Ni, Cr, Co and Mn which occur as contaminations and isomorphic substitutions in most asbestos materials. A direct link between discharge, asbestos fibre and Ni concentrations was demonstrated in the water. Trace metal values in the sediments decrease with distance from the point source but the concentrations 20 km downstream of the slide are still significantly higher than levels at a control station unaffected by the slide. Asbestos fibres leach in acid media, and Mg and trace metals are removed. The process and rates were illustrated on the basis of laboratory experiments using organic acids. Since the pH in the streamwater is decreasing from 8.4 to 7.1 in the downstream direction, trace metals release is of concern.     

LAND USE AND STREAMWATER NITRATE-N DYNAMICS IN AN URBAN-RURAL FRINGE WATERSHED1

ABSTRACT: Agricultural and residential activities are key non-point sources of nitrogen pollution in urban-rural fringe areas. A GIS-based watershed approach was used to compare land use indicators (septic system and animal unit densities), to streamwater nitrate-N in the Salmon River near Vancouver, B.C., Canada. The density of septic systems was used as an indicator of residential development while animal unit density was used as an indicator of the intensity of agricultural activity. Nitrate-nitrogen (nitrate-N) concentrations as high as 7.1 mg·L^?1 were found in the mid-portion of the watershed during the summer months, when streamflow is low and groundwater comprises a large proportion of water in the stream. The major aquifer supplying water to the midsection of the watershed is contaminated with nitrate-N. A comparison of the relationships between septic system and animal unit density and nitrate-N in the upstream to downstream direction provided evidence that both residential and agricultural activities contribute to elevated nitrate-N in the Salmon River mainstem. In contrast, only septic system density corresponded to the pattern of streamwater nitrate-N in Coghlan Creek, the main tributary to the Salmon River.     

Microwave pretreatment for enhancement of phosphorus release from dairy manure

Both the advanced oxidation process (AOP) using a combination of hydrogen peroxide addition and microwave heating (H2O2/microwave), and the microwave heating process were used for solubilization of phosphorus from liquid dairy manure. About 80% of total phosphate was released into the solution at a microwave heating time of 5 min at 170 degrees C. With an addition of H2O2, more than 81% of total phosphate could be released over a reaction period of 49 h at ambient temperature. The AOP process could achieve up to 85% of total phosphate release at 120 degrees C. The results indicated that both the microwave, and the AOP processes could effectively release phosphate from liquid dairy manure. These processes could serve as pretreatments for phosphorus recovery from animal wastes, and could be combined with the struvite crystallization process to provide a new approach in treating animal wastes.     

Chemical Engineering of Enzymes: Altered Catalytic Activity, Predictable Selectivity and Exceptional Stability of the Semisynthetic Peroxidase Seleno-Subtilisin

2  H). As a consequence, the former proteolytic enzyme gained peroxidase activity and catalyzed the selective reduction of hydroperoxides. Due to the identical binding sites of the semisynthetic peroxidase and the protease, the substrate selectivity of seleno-subtilisin was predictable in view of the well-known selectivity of subtilisin.     

Food,feed, and fuelwood resources of Nepal: A GIS evaluation

A GIS-based land evaluation model was developed to determine the food, feed, and fuelwood sufficiency of all districts in Nepal. Resource surpluses or deficits were calculated for each district for 1981, the year for which the national land resource data were available. Of the three resources, feed supplies were found to be the most critical. Feed deficits occurred in 57% of all districts in 1981, while food and fuelwood deficits occurred in less than 10% of all districts. Different scenarios were carried out for the year 2000 to estimate the magnitude of future resource deficits, assuming resource use and yields in Nepal remain constant in the face of growing population and livestock numbers. Linking resource data with spreadsheet and GIS systems provides a new way to understand and evaluate resources at the district and national levels. To more fully capture the usefulness of this approach, information on rates of change in productivity and land use is needed.