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Adenosine triphosphate (ATP) content and carbon dioxide (CO2) production in soil vary considerably, depending on soil type, water content, light, stirring of soil, storage conditions, date of sampling, and chemicals added. In most soils, the biomass calculated on the basis of ATP-content was in line with that calculated on the basis of CO2-production. 相似文献
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The extraction of ATP from soils with the TCA- and NRB ®-methods shows qualitative and quantitative differences. A combination of both methods results in a higher yield of extracted ATP and in a solution of low optical quenching. 相似文献
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Rainer Brüggemann Andreas Kaune Laszlo Zelles Anton Hartmann Christian Steinberg 《Umweltwissenschaften und Schadstoff-Forschung》1995,7(5):265-274
Die ?kotoxikologie mu? sich mit biologischen Systemen und ihren Antworten auf schadstoffinduzierten Stre? auseinandersetzen.
Die hierzu verwendete experimentelle Technik eilt dabei den M?glichkeiten dynamischer, proze?orientierter mathematischer Modellierung
weit voraus.
In dieser methodisch orientierten Arbeit werden Techniken aus der mathematischen Disziplin der Verbandstheorie vorgestellt,
die eine Datenanalyse unter den Aspekten vergleichender Bewertung erlauben. Wichtige Ergebnisse sind dabei
相似文献
– | • Aussagen zur Diversit?t mikrobieller Systeme (soweit durch die hier verwendeten Muster von vier Phospholipidfetts?uren erfa?bar), |
– | • Analysen ordinaler Abh?ngigkeiten sowie |
– | • komprimierte Darstellung komplexer Befunde im Zusammenhang mit der Toxizit?t von Huminstoff-Chemikalien-Systemen. |
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Rainer Brüggemann Andreas Kaune Laszlo Zelles Anton Hartmann Christian Steinberg 《Environmental Sciences Europe》1995,7(5):265-274
Ecotoxicological investigations focus on biological systems and their response to chemically induced stress. Experimental techniques are much more developed than deterministic dynamic modelling. In this methodological contribution a technique is presented, based on lattice theory. This technique, also calledHasse diagram technique, allows data analysis with respect to comparative evaluation. Hasse diagrams are used ? to suggest a possible measure of microbial diversity, ? to analyze dependencies between phospholipid fatty acids and simple geochemical parameters on an ordinal scale and ? to visualise complex results of interactions of humic substances with xenobiotics. 相似文献
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Identification of single cultured micro-organisms based on their whole-community fatty acid profiles, using an extended extraction procedure. 总被引:4,自引:0,他引:4
L Zelles 《Chemosphere》1999,39(4):665-682
Fatty acid profiles obtained from single cultured organisms have been used to estimate which taxonomic groups are actually represented. The lipid extraction was modified to liberate fatty acids with ester-linkages, as well as those with non-ester-linkages and classify them in different chemically relevant groups. The discriminatory power of fatty acids, in different chemically relevant fractions and subfractions varied considerably. Saturated fatty acids were least able to predict actual group membership (ca. 75%), while nonester-linked hydroxy fatty acids, which largely go undetected by the simple extraction procedure, gave the highest predictability values (ca. 94%). The discriminatory power of the method used was enhanced by increasing the number of well-defined fatty acid methyl esters. The estimation capacity of the results was improved, when the fatty acids, which were presumed to be common and widespread, were excluded from the whole-community fatty acid profiles prior to multivariate analysis. 相似文献
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A simple effective procedure for the determination of adenosine triphosphate in soils 总被引:1,自引:0,他引:1
A simple method for the extraction and determination of adenosine 5′-triphosphate (ATP) in soil is described. ATP was extracted by stirring with dimethyl sulphoxide (DMSO), followed by adding 0.01 M trisodium phosphate (Na3PO4) buffer solution for a further dispersion. The ATP content was quantitatively measured by luciferin-luciferase system employing a standard addition technique. The method was most efficient in comparison with 7 other extraction procedures. The recoveries of ATP in spiked soil were found to be approximately 100 %. ATP contents in 16 selected soils were in ranges of 0.76±0.05 – 7.79±0.83 μg/g (dry weight). A significant correlation between ATP amounts and biomasses in these soils was also observed. 相似文献
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