Managing Individuals' Contributions to Maximize the Allelic Diversity Maintained in Small, Conserved Populations

Abstract:  The maintenance of diversity is, from a genetic perspective, one of the key aims in a conservation program. Because the most widely used measure of genetic diversity is the expected heterozygosity of the population, or gene diversity (GD), most research has been devoted to finding optimal strategies for maximizing this parameter. Little attention has been paid, however, to the development of strategies to manage allelic diversity (AD), the number of alleles maintained in the population. Using computer simulations, we show that the strategies that maximize GD, by managing contributions from parents, keep levels of AD as high as strategies maximizing AD itself, for a wide range of situations including different numbers of molecular markers used and the possibility of evaluating a number of offspring per parent to make decisions. Because maximization of GD also minimizes levels of inbreeding, this should be the strategy of choice in any conservationprogram.     

Preserving Population Allele Frequencies in Ex Situ Conservation Programs

Abstract: Optimization of contributions of parents to progeny by minimizing the average coancestry of the progeny is an effective strategy for maintaining genetic diversity in ex situ conservation programs, but its application on the basis of molecular markers has the negative collateral effect of homogenizing the allelic frequencies at each locus. Because one of the objectives of a conservation program is to preserve the genetic composition of the original endangered population, we devised a method in which markers are used to maintain the allele frequency distribution at each locus as closely as possible to that of the native population. Contributions of parents were obtained so as to minimize changes in allele frequency for a set of molecular markers in a population of reduced size. We used computer simulations, under a range of scenarios, to assess the effectiveness of the method in comparison with methods in which contributions of minimum coancestry are sought, either making use of molecular markers or genealogical information. Our simulations indicated that the proposed method effectively maintained the original distribution of allele frequencies, particularly under strong linkage, and maintained acceptable levels of genetic diversity in the population. Nevertheless, contributions of minimum coancestry determined from pedigree information but ignoring the genealogy previous to the conservation program, was the most effective method for maintaining allelic frequencies in realistic situations.     

Conservation Genetics of Potentially Endangered Mutualisms: Reduced Levels of Genetic Variation in Specialist versus Generalist Bees

Abstract:  Oligolectic bees collect pollen from one or a few closely related species of plants, whereas polylectic bees visit a variety of flowers for pollen. Because of their more restricted range of host plants, it maybe expected that specialists exist in smaller, more isolated populations, with lower effective population sizes than generalists. Consequently, we hypothesized that oligolectic bees have reduced levels of genetic variation relative to related polylectic species. To test this hypothesis, we used five phylogenetically independent pairs of species in which one member was oligolectic and the other was polylectic. We assayed genetic variation in our species pairs at an average of 32 allozyme loci. Within each species pair, the oligolectic member had fewer polymorphic loci, lower average allelic richness, and lower average expected heterozygosity than its polylectic relative. Averaged over all species pairs, this corresponds to a 21% reduction in allelic richness, a 72% reduction in the proportion of polymorphic loci, and an 83% reduction in expected heterozygosity in specialists compared with generalists. Our data support the hypothesis of reduced effective population size in oligolectic bees and suggest that they may be more prone to extinction as a result. We suggest that in instances in which bee specialists are involved in mutually codependent relationships with their floral hosts, these mutualisms may be endangered for genetic and ecological reasons.