HISTORICAL TRENDS IN SEDIMENTATION RATES AND SEDIMENT PROVENANCE,FAIRFIELD LAKE,WESTERN NORTH CAROLINA1

Sedimentation rates and sediment provenance were examined for lacustrine sediments deposited in Fairfield Lake, western North Carolina, during the past 111 years. Stratigraphic, radionuclide, and cartographic data indicate that sedimentation rates have increased several fold during the past three decades in response to localized development. The magnitude of increased sedimentation was surprising given limited development within the basin: 0.12 to 0.68 buildings/ha in 2000 in those parts directly delivering sediment to the dated cores. Thus, the analysis illustrates the potential sensitivity of watersheds in the southern Appalachians to changes in land cover. An approach that combined geochemical fingerprinting with sediment mixing models was subsequently evaluated to determine its ability to accurately estimate the contribution of sediment from (1) major bedrock formations that underlie the watershed and (2) potential sources associated with four land cover categories. Sediment sources in both analyses proved difficult to geochemically fingerprint to greater than 90 percent accuracy using data on acid‐soluble metals and selected isotopes of lead (Pb). The relative contributions of sediment from delineated sources, estimated by the mixing models, generally corresponded with known temporal and spatial patterns of land cover. However, the models were plagued by two significant problems — the chemical alteration of sediments as they were transported through upland streams to depositional sites within the lake and the loss of elemental mass. Thus, future investigations using the fingerprinting approach in this area of intense weathering, and presumably others, will need to modify the existing methods to more accurately elucidate changes in sediment provenance related to development.     

Detection of rubella virus in fetal and placental tissues and in the throats of neonates after serologically confirmed rubella in pregnancy

From 35 therapeutic abortions performed because rubella had occurred at 2–19 weeks of pregnancy, 120 fetal organs, 12 specimens of mixed products of conception, and 15 placentae were tested for rubella virus. Virus was isolated from 10 out of 11 fetuses (91 per cent) from women infected at 2–8 weeks, from 5 out of 8 (63 per cent) infected at 9–10 weeks, and from 2 out of 16 (13 per cent) infected at 11–19 weeks. Hybridization tests for viral RNA on 39 fetal organs from eight cases revealed infection in four additional fetuses. Virus was isolated from only 3 out of 15 aborted placentae, but hybridization tests on six placentae revealed infection in three additional specimens. Hybridization was superior to virus isolation for detecting rubella infection in products of conception and is therefore potentially the better method for examining chorionic villus biopsies. Rubella virus was isolated from the throats of 4 out of 9 infants (44 per cent) infected during the first 12 weeks of gestation, but from none of 13 infected after 17 weeks. Infants in the latter group are unlikely to infect susceptible contacts.     

Fetoscopic myelomeningocoele closure: Is the scientific evidence enough to challenge the gold standard for prenatal surgery?

Since the completion of the Management of Myelomeningocoele Study, maternal-fetal surgery for spina bifida has become a valid option for expecting parents. More recently, multiple groups are exploring a minimally invasive approach and recent outcomes have addressed many of the initial concerns with this approach. Based on a previously published framework, we attempt to delineate the developmental stage of the surgical techniques. Furthermore, we discuss the barriers of performing randomized controlled trials comparing two surgical interventions and suggest that data collection through registries is an alternative method to gather high-grade evidence.     

A simple economic model of endangered species preservation in the United States

Concern for the acceleration in threats to species diversity in the United States led to the passage of the Endangered Species Act of 1973. In this paper, this legislation is examined in an economic context. A simple model is developed where management and constraints on economic activity are substitutes in the production of species stocks. Whether economic growth constraints (the primary policy tool of the 1973 Act) are justified for preservation purposes depends on technical substitution possibilities, relative cost ratios, and the existence of budget constraints for species management. Since the same combination of these factors will not occur for all species, it is argued that more flexibility in endangered species policy may preserve the same levels of species stocks at lower costs to society.     

Succession in sea urchin and seaweed abundance in Nova Scotia,Canada

From 1980 through 1983 sea urchin mass mortalities were at least 245 000 t. The habitat released to seaweed is expected to support a standing crop of 1.8 million tons and an annual production of 7 million tons. An extensive survey of 2 900 km of Nova Scotia shoreline revealed that 511 km² of habitat (rock bottom less than 15 m deep) was available to seaweeds. Areas most exposed to ocean swells bordered 26% of the shoreline length but included 82% of the seaweed habitat. Before mass mortalities, seaweed refuges from sea urchin grazing included very sheltered habitats, very exposed habitats, and boulders set among shifting sand; however, these were less than 10% of the total habitat. In the 1 to 2 years following mass mortalities seaweed cover and occurrence increased significantly for all of five categories of wave exposure and for six of seven algal taxa respectively. In at least three locations the sea urchin population began to recover as a result of larval recruitment but in 1983 was again reduced by disease. Sea urchins appear to lack natural resistance to disease, at least at warm temperatures. In the laboratory all sea urchins collected from the Bay of Fundy, southern Nova Scotia, eastern Newfoundland, and an area recovering from mass mortalities died following exposure to diseased animals. Also, field mortalities included a wide variety of habitats. Interviews of Nova Scotia lobster fishermen revealed that sea urchin mass mortalities have probably occurred before in this century, but infrequently.     

Characterization of two glutamate dehydrogenases from the symbiotic microalga Symbiodinium microadriaticum isolated from the coral Acropora formosa

In view of their possible involvement in ammonium assimilation in the coral/algal symbiosis, we have purified two distinct glutamate dehydrogenase isoenzymes from the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal) extracted from the staghorn coral Acropora formosa collected from Magnetic Island, North Queensland, Australia, in 1986–1987. An NADPH-specific glutamate dehydrogenase (GDH) displayed biphasic kinetics with respect to ammonium as the variable substrate; at low substrate concentrations the apparent K _m was below 1 mM, whereas at high substrate concentrations the corresponding value was approximately 200 mM. The NADPH-GDH displayed extremely low activity in the direction of glutamate oxidation; together with the kinetic data this suggests a probable role in ammonium assimilation. A second (NADH-specific) GDH was found to have both amination and deamination activities, and presumably functions in vivo in glutamate oxidation. Kinetic constants are reported for both GDH isoenzymes.     

Early life history of tropical Anguilla leptocephali in the western Pacific Ocean

In order to examine the early life-history characteristics of tropical eels, otolith microstructure and microchemistry were examined in leptocephali of Anguilla bicolor pacifica (27.6-54.1 mm TL, n=20) and A. marmorata (22.0-47.3 mm TL, n=8) collected during a cruise in the western Pacific. A. bicolor pacifica occurred between 10°N and 15°N in the west and between 5°S and 10°N farther to the east. A. marmorata also occurred in two different latitudinal ranges in the Northern (15-16°N) and Southern Hemispheres (3-15°S) of the western Pacific. The increment widths in the otoliths of these leptocephali increased between the hatch check (0 days) and about an age of 30 days in both species, and then gradually decreased toward the otolith edge. Otolith Sr:Ca ratios showed a gradual increase from the otolith center to the edge. The ages of A. bicolor pacifica and A. marmorata leptocephali ranged from 40 to 128 days and from 38 to 99 days, respectively. Growth rates of A. bicolor pacifica and A. marmorata leptocephali ranged from 0.33 to 0.71 mm day^-1 and from 0.45 to 0.63 mm day^-1, respectively. These leptocephali had estimated growth rates that were spread out throughout most of the reported range of growth rates of the leptocephali of the temperate species, the Japanese eel and the Atlantic eels. Differences in the spatial distribution in relation to current systems, and the age and size compositions of the leptocephali of A. bicolor pacifica and A. marmorata suggested different spawning locations for these two species.     

Successful Deployment of an Innovative Environmental Technology: The Terra-Kleen Experience

The National Research Council's report, Innovations in Ground Water and Soil Cleanup: From Concept to Commercialization, reviewed the implementation of innovative remediation technologies and portrayed a bleak outlook for them. Conversely, Terra-Kleen has invented, patented, permitted, and profited through the use of an innovative technology in the current market. Terra-Kleen has implemented a solvent extraction technology for the removal of PCBs, PAHs, and chlorinated pesticides from soil, sediment, and debris. To date, Terra-Kleen has successfully processed over 23 million pounds of PCB-contaminated soil in Alaska, California, Florida, Ohio, and Oklahoma. The company has been profitable each year since its inception, and has averaged a revenue growth rate of 94 percent per year for the last four years. Corporate growth has been financed entirely through profits and conventional bank loans at favorable interest rates. This article reviews the factors that have led to Terra-Kleen's success, overviews the solvent extraction technology, and details specific projects that have implemented the technology.