Subpopulations of the Hawaiian striped mullet Mugil cephalus: analysis of variations of nuclear eye-lens protein electropherograms and nuclear eye-lens weights

The method of electrophoretic separation of nuclear eye-lens proteins was applied to a study of the population structure of the striped mullet Mugil cephalus L. in Hawaii. Electropherograms produced 5 pattern types, with most variations due to non-developmental genetic polymorphism. Some variations, however, were associated with size, and an ontogenetic basis was considered. The frequencies of the pattern types were significantly (p<0.005) independent among the three geographical subpopulations examined: Kaneohe Bay, Oahu; Diamond Head, Oahu; and Kawaihae Bay, Hawaii. Growth rates of mullet eye-lens nuclei demonstrated significant (p<0.05) differences between sexes and localities, except for Kaneohe Bay males versus Diamond Head males, which were nearly significant (p 0.075). These variations in eye-lens nuclei growth rates are probably regulated by genetic factors. Nearly complete genetic isolation is evident for the three subpopulations of mullet in the Hawaiian Islands. The occurrence of genetic interchange in apparently less frequent between the Oahu and Hawaii populations than between the two Oahu populations, in accordance with their respective geographical distances.Oceanic Institute Contribution No. 72.     

Synthesis and biological activities of new azacrown ether Schiff bases and spectrophotometric studies of their complexation with [60]fullerene

A series of novel azacrown ether Schiff bases 1–3 have been synthesized in good yield and in a simple way. Their host–guest interaction with [60]fullerene has been studied in toluene by absorption spectroscopic method. All the complexes are found to be stable with 1:1 stoichiometry. Because of their potential applications in industry, agriculture and medicine, they were investigated for their mutagenic and antimutagenic activities using the spot test and the plate incorporation assay of Ames. Compounds 1, 2 and 3 were found to be nonmutagenic in the Ames test using strains TA 1535, TA100 and TA97a of Salmonella typhimurium. However, using strain TA102 revealed that, although both compounds 1 and 2 were nonmutagenic, compound 2 gave a positive response indicating that it acts as an oxidative mutagen. The structure-activity relationship may throw some light on the biological activity of such series of compounds.