Salmonella as an index of pollution of fresh-water environments

An environmental study was done to examine the prevalence of Salmonella in some aquatic environments of Jabalpur. Environmental factors in the fresh-water environment exert an influence on the distribution and behaviour of pathogenic bacteria. During the period from January 1991 to December 1992, a total number of 103 isolates of Salmonella were tested for their resistance against antibiotics. Among different isolates, Salmonella paratyphii showed 100% multiple resistance against antibiotics, i.e. Ampicillin, Carbenicillin, Cephalexin, Chloramphenicol, Gentamicin, Penicillin, Streptomycin, Tetracycline, Norfloxacine and Cloxacine. The density of Salmonella correlated with the densities of total coliforms, faecal coliforms and faecal streptococci. The increased survival, possible indigenous nature and behaviour of Salmonella further emphasize the need for direct enumeration, reformation of standards and health risk assessments for underdeveloped countries, where waterborne disease exert a horrible toll.     

Classification of floristic composition of mangrove forests using hyperspectral data: case study of Bhitarkanika National Park, India

The aim of the present work is to unveil the potential of some of the unexplored remote sensing techniques for mangrove studies. The paper deals with the classification of an Earth Observing–1 Hyperion image of the mangrove area of Bhitarkanika National Park, Odisha, India into mangrove floristic composition classes. Out of 196 calibrated bands of the image, 56 were found to be highly uncorrelated and contained maximum information; therefore, these 56 bands were used for classification. Amongst the three full–pixel classifiers tested in the investigation, Support Vector Machine produced the best results in terms of training pixel accuracy with overall precision of 96.85 %, in comparison to about 70–72.0 % for the other two classifiers. A total of five mangrove classes were obtained – pure or dominant class of Heritiera fomes, mixed class of H. fomes, mixed Excoecaria agallocha with Avicennia officinalis, mixed class of fringing Sonneratia apetala and class comprising of mangrove associates with salt resistant grasses. Post–classification field data also established the same. Pure or dominant classes of H. fomes occupied more than 50 % of the total mangrove vegetation in the forest blocks of the National Park. Spectral profile matching of image pixels with that of in–situ collected canopy reflectance profile revealed good match for H. fomes (pure or dominant stands). Red–edge index, which was a preferred criterion for matching was notably correlated in case of H. fomes and E. agallocha. The outcomes indicated the efficacy of hyperspectral canopy reflectance library for such kind of work. It is hoped that the methodology presented in this paper will prove to be useful and may be followed for producing mangrove floristic maps at finer levels.     

Assessment of deposition of MEMC (seed dressing fungicide) in exposed rice grain through study of seedling growth parameters

Rice seed grains exposed to 240 ppm of seed dressing fungicide, MEMC showed increase in deposition of mercury with increase in period of observation (0h to 48h) in 765+/-5 mg of intact endosperm. The gradual increase in mercury deposition in intact endosperm caused simultaneous decrease in mercury content of the seed coat. Exposure periods of 5 to 20 min with 240 ppm of MEMC showed 5.5 to 8.72 microg of mercury deposition in 765+/-5 mg of intact endosperm. Increase in period of exposure (3 to 20 min) to 240 ppm of MEMC and also increase in chronic treatment to lower concentrations (1.5 ppm to 24 ppm) to seed grains have shown similar results in seedling growth parameters. Lower period of exposure or exposure to lower concentrations, both showed a significant inhibition of root growth, however the shoot growth showed stimulation. But higher exposure period or exposure to higher concentrations brought inhibition of both root and shoot growth. The exposure period showing 50% of root growth inhibition of 72h old seedling was calculated to be 10 min So also the chronic treatment showing the 50% of root growth inhibition was calculated to be 9 ppm. Ten min exposure of rice grain to 240 ppm of MEMC showed deposition of 7.11 microg of mercury in 765+/-5 mg of intact endosperm at 48h of observation (optimal phase of germinating process). Regression analysis indicated that by the time of 72h the intact endosperm and developing seedling might have acquired with a minimum amount of 10.23 microg of mercury from the seed coat. Finally it can be said that 10.23 microg of MEMC might be bringing 50% root growth inhibition in 72h old seedlings and 16.71 microg of mercury causing 50% inhibition of both root and shoot growth of 120h old seedlings.     

Soil dehydrogenase, phosphomonoesterase and arginine deaminase activities in an insecticide treated groundnut (Arachis hypogaea L.) field

Chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2 pyridyl phosphorothioate) 20 EC and Quinalphos (O,O-diethyl O-quinoxalin-2-yl phosphorothioate) 25 EC, were applied in groundnut (Arachis hypogaea L.) field as seed treatment at 25 ml/kg and soil treatment at 4 l/ha in 1998 and 1999. The residues of these insecticides were monitored during the entire crop season and their effect on the soil enzymes dehydrogenase, phosphomonoesterase and arginine deaminase were studied. Ninety nine percent of chlorpyrifos residues were dissipated within 60 days from seed treated soil and 98% dissipation was observed in soil treated field for the same days. Its half lives in seed treated soil were 8 days and 7 days and in soil treated field were 9.2 days in and 7.5 days in 1998 and 1999 respectively. Dissipation of quinalphos in comparison to chlorpyrifos was slow both in seed treated and soil treated field. Eighty seven percentage to 92% dissipation of quinalphos residues were observed from seed treated soil and 98% residues were dissipated from soil treated field within 75 days. Its half lives in seed treated soil were 20 days and 18 days and in soil treated field, its half lives were 13 days and 17 days 1998 and 1999 respectively. Inhibition in dehydrogenase activity followed by recovery was observed both in seed and soil treatments with chlorpyrifos. An inhibition of 17.2% was estimated after 60 days of seed treatment in comparison to control. Dehydrogenase activity was significantly reduced to 63% after 15 days of quinalphos seed treatment in comparison to control in 1998. Similar trends were observed in 1999. A significant inhibition in dehydrogenase activity was observed after soil treatment both in 1998 and 1999. Phosphomonoesterase activities were significantly inhibited upto 25.2% as compared to the control, on the 15th day of chlorpyrifos seed treatment in 1998 and similarly, after one day of treatment in 1999. Quinalphos inhibited the phosphomonoesterase activity till the end of the experimental period in the soil treated fields, whereas recovered within 30-60 days of treatment in the seed treated fields. Arginine deaminase activity was significantly stimulated within one day after chlorpyrifos seed and soil treatments in both years. The activity was almost threefold higher on the 30th and the 15th day of soil treatment in 1998 and 1999, respectively. A temporary inhibition of arginine deaminase activity was observed after quinalphos treatment. It was observed that in most of cases insecticides have temporary inhibitory effect on soil enzymes. However, inhibition was smaller in seed treated soil than in direct soil treatment.     

Bacopa phospholipid complex retrieves aluminum maltolate complex–induced oxidative stress and apoptotic alterations in the brain regions of albino rat

Environmental Science and Pollution Research - Highly bioavailable plant phospholipid complex that can reverse aluminum maltolate (AlM)–induced toxicity is not yet reported. Hence, the...     

Bacterial contamination in drinking water: a case study in rural areas of northern Rajasthan, India

Acid deposition has caused detrimental effects on tree growth near industrial areas of the world. Preliminary work has indicated that concentrations of NO(3-), SO(4)(2-), F( - ) and Al in soil solutions were 2 to 33 times higher in industrial areas compared to non-industrial areas in Korea. This study evaluated soil nutrient bioavailability and nutrient contents of red pine (Pinus thunbergii) needles in forest soils of industrial and non-industrial areas of Korea. Results confirm that forest soils of industrial areas have been acidified mainly by deposition of sulfate, resulting in increases of Al, Fe and Mn and decreases of Ca, Mg and K concentrations in soils and soil solutions. In soils of industrial areas, the molar ratios of Ca/Al and Mg/Al in forest soils were <2, which can lead to lower levels and availability of nutrients for tree growth. The Ca/Al molar ratio of Pinus thunbergii needles on non-industrial sites was 15, while that of industrial areas was 10. Magnesium concentrations in needles of Pinus thunbergii were lower in soils of industrial areas and the high levels of acid cations such as Al and Mn in these soils may have antagonized the uptake of base cations like Mg. Continued acidification can further reduce uptake of base cations by trees. Results show that Mg deficiency and high concentrations of Al and Mn in soil solution can be limiting factors for Pinus thunbergii growth in industrial areas of Korea.     

Environmental conditions of Chilika Lake during pre and post hydrological intervention: an overview

Chilika Lake, the largest brackish water lagoon in Asia, is a prominent biodiversity hotspot along the Indian east coast. The geomorphology, water quality and biological productivity of the lake had undergone significant changes over the years under the influence of natural events and anthropogenic interventions. Decades of research have shown that the ecological changes of the lake system and its fisheries were influenced mainly by the water exchange between the lake and the sea that controls the salinity, siltation, macrophyte infestation and recruitment of marine forms. Depletion of fisheries and loss of biodiversity in 1980s and 1990s have been linked to the northward shifting of lake’s inlet and silting up of the outer channel. In order to improve the health of the lagoon and restoration of its biodiversity, a new mouth was dredged open in September 2000. Opening of the new mouth has resulted in some improvements with substantial increase in capture fishery, reduction in weed infestation, growth of seagrasses, appearance of dolphins and increase in the population of migratory birds. But, many stake holders claim that the problems associated with the lake ecology and its contribution to the socio-economic development of the stake holders still persist which could escalate in future.     

Carbon density and accumulation in agroecosystem of Indo-Gangetic Plains and Vindhyan highlands,India

In agroecosystems, the annual carbon (C) inputs to soil are one of the most promising greenhouse gas mitigation options. Net primary productivity (NPP) provides the inputs of C in ecosystems that can potentially be sequestered in soil organic matter. In this study, we estimates the C density and accumulation rate in rice–wheat agroecosystem at four sites of Indo-Gangetic Plains and Vindhyan highlands through amalgamation of ground truth (GT) and remote sensing (RS) approach. In addition to this, we validated field-measured aboveground net production (ANP) with remotely sensed SPOT-Vegetation data. ANP of the sites ranged from 6.8 to 11.1 and 3.3 to 8.8 t-C ha^?1 year^?1 for GT and RS, respectively. Both estimates (GT and RS) were linearly and significantly related with each other (y?=?1.33x???5.82, R ²?=?0.93, P?=?0.04; where x?=?GT ANP and y?=?RS ANP). For the whole region, total NPP (rice?+?wheat) was ranged from 7.9 to 12.5 t-C ha^?1 year^?1. The C accumulation potential of the present agroecosystems was 9.1 and 1.9 t-C ha^?1 year^?1 in the form of ANP and belowground NPP (BNP), respectively. The aboveground C stock of agroecosystem allocated in foliage (7.8 %), stem (57.6 %), and grain (35.1 %). Survey about the fate of post-harvested materials indicated that aboveground portion of the crop is almost used completely within the year for different purposes including human consumption and cattle feeding. Therefore, only BNP and litter contribute to long-term C sequestration. Since agroecosystem has enormous potential to sequester C, better management of aboveground portion NPP of the crop may enhance C sequestration potential of agroecosystem.