Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil

The use of filamentous fungi in bioremediation of heavy metal contamination has been developed recently. This research aims to observe the capability of filamentous fungi isolated from forest soil for bioremediation of mercury contamination in a substrate. Six fungal strains were selected based on their capability to grow in 25 mg/L Hg2+-contaminated potato dextrose agar plates. Fungal strain KRP1 showed the highest ratio of growth diameter, 0.831, thus was chosen for further observation.Identification based on colony and cell morphology carried out by 18S rRNA analysis gave a 98%match to Aspergillus flavus strain KRP1. The fungal characteristics in mercury(Ⅱ) contamination such as range of optimum pH, optimum temperature and tolerance level were 5.5–7 and 25–35℃ and 100 mg/L respectively. The concentration of mercury in the media affected fungal growth during lag phases. The capability of the fungal strain to remove the mercury(Ⅱ) contaminant was evaluated in 100 mL sterile 10 mg/L Hg2+-contaminated potato dextrose broth media in 250 mL Erlenmeyer flasks inoculated with 108spore/mL fungal spore suspension and incubation at 30℃ for 7 days. The mercury(Ⅱ) utilization was observed for flasks shaken in a 130 r/min orbital shaker(shaken) and nonshaken flasks(static) treatments. Flasks containing contaminated media with no fungal spores were also provided as control. All treatments were done in triplicate. The strain was able to remove 97.50%and 98.73% mercury from shaken and static systems respectively. A. flavus strain KRP1 seems to have potential use in bioremediation of aqueous substrates containing mercury(Ⅱ) through a biosorption mechanism.     

Mitigation of greenhouse gas emissions from an abandoned Baltic peat extraction area by growing reed canary grass: life-cycle assessment

Abandoned peat extraction areas are continuous emitters of GHGs; hence, abandonment of peat extraction areas should immediately be followed by conversion to an appropriate after-use. Our primary aim was to clarify the atmospheric impact of reed canary grass (RCG, Phalaris arundinacea L.) cultivation on an abandoned peat extraction area and to compare it to other after-treatment alternatives. We performed a life-cycle assessment for five different after-use options for a drained organic soil withdrawn from peat extraction: (I) bare peat soil (no management), (II) non-fertilised Phalaris cultivation, (III) fertilised Phalaris cultivation, (IV) afforestation, and (V) rewetting. Our results showed that on average the non-fertilised and fertilised Phalaris alternatives had a cooling effect on the atmosphere (?10,837 and ?477 kg CO₂-eq ha^?1 year^?1, respectively), whereas afforestation, rewetting, and no-management alternatives contributed to global warming (9,511, 8,195, and 2,529 kg CO₂-eq ha^?1 year^?1, respectively). The main components influencing the global warming potential of different after-use alternatives were site GHG emissions, carbon assimilation by plants, and emissions from combustion, while management-related emissions played a relatively minor role. The results of this study indicate that, from the perspective of atmospheric impact, the most suitable after-use option for an abandoned peat extraction area is cultivation of RCG.     

The role of migration and founder effect for the evolution of cooperation in a multilevel selection context

The idea that natural selection can be meaningfully applied at the group level may be more important than previously thought. This perspective, a modern version of group selection, is called multilevel selection. Multilevel selection theory could incorporate previous explanations for the evolution of cooperation including kin selection. There is general agreement that natural selection favors noncooperators over cooperators in the case of an unstructured population. Therefore, the evolution of cooperation by multilevel selection often requires positive assortment between cooperators and noncooperators. The question is how this positive assortment can arise in the ecological meaning. We constructed an individual-based model of multilevel selection and introduced migration and evolution. The results showed that positive assortment was generated especially when a migration strategy was adopted in which individuals respond specifically to bad environmental conditions. It was also shown that the founder effect in the evolutionary process could further facilitate positive assortment by working with migration. We analyzed assortment by using relatedness defined in group-structured populations. The fact that cooperation was achieved by such migration and by the founder effect highlights the importance of sensitiveness to the ecological environment and of fluctuations in group size, respectively.     

Fluoride in drinking water exacerbates glomerulonephritis and induces liver damage in ICR-derived glomerulonephritis mice

To evaluate the effects of fluoride on the kidney and the liver of ICR-derived glomerulonephritis (ICGN) mice by using laboratory tests and pathological examinations, fluoride was administered to the ICGN mice at 0, 25, 50, 100, and 150?ppm in drinking water for 4 weeks and to the ICR mice, which have normal kidney function at 0 and 150?ppm. The BUN, creatinine, GOT, and GPT in the serum of each mouse were determined. When a mouse died, the sample from the day closest to the death was assigned for the mean. Pathological changes in the kidney were examined after PAS (periodic acid-Schiff) staining. All of the ICGN mice in the 150?ppm group and one of seven in the 100?ppm group died before the end of week 4, but no ICR mice died. For ICGN mice, the mean value of body weight in the 150?ppm group was significantly lower than those in 0?ppm group and other fluoride-administered groups. The mean values of relative liver and kidney weights in the 100 and 150?ppm groups were significantly lower than those in the control. The mean values of BUN, creatinine, and GPT in the 150?ppm group were significantly higher than those in the control. The thickness of the glomerular capillary wall and the increased mesangial matrix in the kidney were prominent in the fluoride-administered ICGN mice. These results suggested that fluoride severely exacerbated glomerulonephritis and tublar-intestitial changes in ICGN mice.     

Response surface method for modeling the removal of carbon dioxide from a simulated gas using water absorption enhanced with a liquid-film-forming device

This paper presents the results from using a physical absorption process to absorb gaseous CO_2mixed with N_2using water by producing tiny bubbles via a liquid-film-forming device(LFFD)that improves the solubility of CO_2in water.The influence of various parameters—pressure,initial CO_2concentration,gas-to-liquid ratios,and temperature—on the CO_2removal efficiency and its absorption rate in water were investigated and estimated thoroughly by statistical polynomial models obtained by the utilization of the response surface method(RSM)with a central composite design(CCD).Based on the analysis,a high efficiency of CO_2capture can be reached in conditions such as low pressure,high CO_2concentration at the inlet,low gas/liquid ratio,and low temperature.For instance,the highest removal efficiency in the RSM–CCD experimental matrix of nearly 80%occurred for run number 20,which was conducted at 0.30 MPa,CO_2concentration of 35%,gas/liquid ratio of 0.71,and temperature of 15°C.Furthermore,the coefficients of determination,R~2,were 0.996 for the removal rate and 0.982 for the absorption rate,implying that the predicted values computed by the constructed models correlate strongly and fit well with the experimental values.The results obtained provide essential information for implementing this method properly and effectively and contribute a promising approach to the problem of CO_2capture in air pollution treatment.     

Delignification of disposable wooden chopsticks waste for fermentative hydrogen production by an enriched culture from a hot spring

Hydrogen(H2) production from lignocellulosic materials may be enhanced by removing lignin and increasing the porosity of the material prior to enzymatic hydrolysis. Alkaline pretreatment conditions,used to delignify disposable wooden chopsticks(DWC) waste, were investigated. The effects of NaOH concentration, temperature and retention time were examined and it was found that retention time had no effect on lignin removal or carbohydrate released in enzymatic hydrolysate. The highest percentage of lignin removal(41%) was obtained with 2% NaOH at 100℃, correlated with the highest carbohydrate released(67 mg/gpretreated DWC) in the hydrolysate. An enriched culture from a hot spring was used as inoculum for fermentative H2 production, and its optimum initial pH and temperature were determined to be 7.0 and 50℃, respectively. Furthermore, enzymatic hydrolysate from pretreated DWC was successfully demonstrated as a substrate for fermentative H2 production by the enriched culture. The maximum H2 yield and production rate were achieved at 195 mL H2/g total sugarsconsumedand 116 mL H2/(L·day), respectively.     

Inactivation effect of pressurized carbon dioxide on bacteriophage Qβ and ΦX174 as a novel disinfectant for water treatment

The inactivation effects of pressurized CO2 against bacteriophage Qβ and ΦX174 were investigated under the pressure of 0.3–0.9 MPa, initial concentration of 107–109PFU/mL, and temperature of17.8°C–27.2°C. The optimum conditions were found to be 0.7 MPa and an exposure time of 25 min. Under identical treatment conditions, a greater than 3.3-log reduction in bacteriophage Qβ was achieved by CO2, while a nearly 3.0 log reduction was observed for phage ΦX174. The viricidal effects of N2O(an inactivation gas with similar characteristics to CO2), normal acid(HCl), and CO2 treatment with phosphate buffered saline affirmed the chemical nature of CO2 treatment. The pumping cycle, depressurization rate, and release of intracellular substances caused by CO2 were its viricidal mechanisms. The results indicate that CO2 has the potential for use as a disinfectant without forming disinfection by-products.     

Examining farmland applications of composted biosolid wastes depending on nutrient balance in soils

This study emphasizes nutrient balance of soils in the farmland application of composted biosolid wastes. The loading rates of plant nutrients following the compost application to farmland in Japan were estimated and compared with the nutrient uptake of agricultural plants. Results show that the current compost application in Japan can meet the requirements of agricultural plants for plant nutrient Ca, except for K, Mg, P, Fe, Mn, Cu, and Zn. The compost application could realize the safe disposal of biosolid wastes and the effective recycling of plant nutrients in composts to soils without causing heavy metal accumulation. The application manner of composts affects the heavy metal accumulation in farmlands. Field examination indicates that the excessive compost application has led to the heavy metal accumulation in compost-amended farmlands. Measuring the nutrient balance in compost-amended farmlands is well suitable for explaining the accumulation of heavy metals, such as Cu and Zn.