Acetylcholinesterase-polyaniline biosensor investigation of organophosphate pesticides in selected organic solvents

The behavior of an amperometric organic-phase biosensor consisting of a gold electrode modified first with a mercaptobenzothiazole self-assembled monolayer, followed by electropolymerization of polyaniline in which acetylcholinesterase as enzyme was immobilized, has been developed and evaluated for organophosphorous pesticide detection. The voltammetric results have shown that the formal potential shifts anodically as the Au/MBT/PANI/AChE/PVAc thick-film biosensor responded to acetylthiocholine substrate addition under anaerobic conditions in selected organic solvent media containing 2% v/v 0.05 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Detection limits in the order of 0.147 ppb for diazinon and 0.172 ppb for fenthion in acetone-saline phosphate buffer solution, and 0.180 ppb for diazinon and 0.194 ppb for fenthion in ethanol-saline phosphate buffer solution has been achieved.     

Achieving uniform regulation of environmental lead exposure and poisoning in wildlife and humans

Lead from spent ammunition causes preventable lead exposure in wildlife and humans that may ingest it. Nontoxic substitutes for lead ammunition exist but are not adopted widely because of hunter opposition. Other forms of lead exposure in the human environment have been heavily regulated because there is no safe level of exposure. The use of lead ammunition should be regulated similarly to protect wildlife from this common disease.     

Rehabilitation of the chernoistochinskii drinking water system,Nizhnii Tagil,Russia

The U.S. Environmental Protection Agency (EPA) performed a reservoir diagnosis and developed low-cost recommendations to improve drinking water quality for the city of Nizhnii Tagil, Russia. Despite widespread regional pollution, water quality within the drinking water supply source can be summarized as generally good. Sampling and analysis efforts did not reveal appreciable amounts of hazardous chemicals or metals. The major water quality problem stems from an abundance of blue-green algae during the summer months, causing drinking water taste and odor problems and filter clogging at the water treatment plant. City water treatment officials also frequently experience difficulties with maintaining an effective disinfection residual in the distribution system because it spans more than 30 kilometers in length. Regrowth of microorganisms in the distribution system often causes coliform bacteria counts to exceed standards. The implementation of a watershed protection and management program, and the installation of a new disinfection system, to purify drinking water and to reduce bacterial growth in the distribution system should improve drinking water quality for the city residents.     

Reflecting ecological criteria in laws supporting the Baja to Bering Sea marine protected areas network case study

Canadian and US marine conservation law, and other related law, was analyzed to determine if it reflected ecological criteria needed to implement connectivity among marine protected areas of the northeast Pacific in the proposed trilateral Baja to Bering Sea (B2B) initiative. The analysis included both nations’ federal laws and those of California, Oregon, Washington, Alaska, and British Columbia. While legal provisions exist already to implement marine protected areas for varying reasons, there is little capacity in most laws to create connectivity among them for conservation purposes. Only California's legislation contained explicit provisions for all the criteria. Other federal, state, and provincial laws, while containing provisions for species at risk and vulnerable habitats, generally lacked explicit provisions for the vital criteria, size of area, migratory patterns, and recruitment patterns. Implementation, future management, and protection of the proposed B2B marine network would be facilitated by amendment of both Canadian and US laws. Some of the ecological criteria are already implied implicitly or vaguely, but they need to be made explicit in the amended law. The legislative model of California could serve as a template for amending the laws of other jurisdictions in the B2B venture.     

Restoration and protection of aqueous environments by utilizing aquatic organisms

Summary Past and current uncontrolled dumping, land application and accidental spills of recalcitrant, toxic environmental pollutants such as DDT and polychlorinated biphenyls (PCBs) pose a continued world-wide environmental threat, in particular to aquatic environments. Bioaccumulative contaminants are rapidly absorbed out of water-borne ambient environments and concentrated in the tissues of living aquatic organisms at concentrations that can range from thousands to millions of times greater than levels in the ambient environment. These absorbed levels are high enough to cause dysfunction in the organisms and potential harmful effects to humans. An established technology capable of remediating the low contaminant levels originating in the ambient aquatic environment does not currently exist. This paper proposes the macro-bioremediation process whereby certain fish and other macroscopic aquatic organisms could be used to filter, concentrate and remove bioaccumulative contaminants from polluted aqueous systems. Contaminant removal would involve the harvesting and subsequent restocking of aquatic organisms capable of bioaccumulating high contaminant levels in relatively short time periods. Tissues of harvested organisms could be composted with specialized fungus and bacteria to fully degrade the recalcitrant contaminants. The macro-bioremediation process could be used at numerous geographic locations for the restoration of natural aquatic environments, supplemental wetlands treatment and for waste-water, hazardous waste and sludge treatment augmentation.     

Metal mobility during in situ chemical oxidation of TCE by KMnO4

The potential for trace-metal contamination of aquifers as a side effect of In Situ Chemical Oxidation (ISCO) of chlorinated solvent contamination by KMnO(4) is investigated with column experiments. The experiments investigate metal mobility during in situ chemical oxidation of TCE by KMnO(4) under conditions where pH, flow rate, KMnO(4), TCE, and trace-metal concentrations were controlled. During ISCO, the injection of MnO(4) creates oxidizing conditions, and acidity released by the reactions causes a tendency toward low pH in aquifers. In order to evaluate the role of pH buffering on metal mobility, duplicate columns were constructed, one packed with pure silica sand, and one with a mixture of silica sand and calcite. Aqueous solutions of TCE and KMnO(4) (with 1 mg/L Cu, Pb, Zn, Mo, Ni, and Cr(VI)) were allowed to mix at the inlet to the columns. After the completion of the experiments, samples of Mn oxide were removed from the columns and analyzed by analytical scanning and transmission electron microscopy. In order to relate the results of the laboratory experiments to field settings, the analyses of Mn-oxide samples from the lab experiments were compared to samples of Mn oxide collected from a field-scale chemical-oxidation experiment that were also analyzed by analytical electron microscopy as well as time-of-flight secondary-ion mass spectroscopy. The pH ranged from 2.40 in the silica sand column to 6.25 in the calcite-containing column. The data indicate that aqueous Mo, Pb, Cu and Ni concentrations are attenuated almost completely within the columns. In contrast, Zn concentrations are not significantly attenuated and Cr(VI) is transported conservatively. The results indicate that within the range 2.40 to 6.25, metal mobility is not affected by pH. Comparison of analyses of Mn-oxide from the lab and field demonstrate that a variety of metals are sequestered from solution by Mn oxide.     

Lead-Free Hunting Rifle Ammunition: Product Availability, Price, Effectiveness, and Role in Global Wildlife Conservation

Proposals to end the use of lead hunting ammunition because of the established risks of lead exposure to wildlife and humans are impeded by concerns about the availability, price, and effectiveness of substitutes. The product availability and retail prices of different calibers of lead-free bullets and center-fire rifle ammunition were assessed for ammunition sold in the USA and Europe. Lead-free bullets are made in 35 calibers and 51 rifle cartridge designations. Thirty-seven companies distribute internationally ammunition made with lead-free bullets. There is no major difference in the retail price of equivalent lead-free and lead-core ammunition for most popular calibers. Lead-free ammunition has set bench-mark standards for accuracy, lethality, and safety. Given the demonstrated wide product availability, comparable prices, and the effectiveness of high-quality lead-free ammunition, it is possible to phase out the use of lead hunting ammunition world-wide, based on progressive policy and enforceable legislation.     

A Device for Measuring Volatile Organic-Carbon Emissions from Cooling-Tower Water

A manual method for measuring reduced sulfur compounds in kraft pulp mill and sulfur recovery plant emissions was evaluated. The method involves removing SO₂ from the gas stream (if present) with a citric acid-potassium citrate buffer that passes reduced sulfur compounds; thermal oxidation of all reduced sulfur compounds to SO₂; collection of the SO₂ in H₂O₂; and a titrimetric analysis of the H₂O₂ for SO₄ ^2?. A heated filter removes alkaline particulate matter that would produce a negative interference if absorbed by the buffer. When used at kraft pulp mills, the method agrees closely with Reference Method 16, provided that nonregulated reduced sulfur compounds, such as carbonyl sulfide, are not present in the emissions. At sulfur recovery plants, nonregulated reduced sulfur compounds, such as thiophene, are likely to be present in the emissions and will produce a positive bias in the results obtained with this method. The precision of the method ranges from 1 to 7 percent relative standard deviation.     

Prenatal diagnosis of vascular anomalies

Vascular anomalies are rare disorders that encompass a group of lesions characterized by abnormal development of the lymphovascular system. Majority of these anomalies are present at birth and could potentially be detected during the prenatal period on imaging. This allows for early intervention and prompt management to improve outcomes. However, they can be difficult to diagnose, given the rarity and overlapping findings. In this review article, we provide a comprehensive overview of congenital vascular anomalies with a liberal use of images of recent cases at our center emphasizing prenatal imaging findings and the natural history of these conditions.