Behavioural early warning responses to polluted water

Behavioural early warning responses to polluted surface water from an industrial effluent have been measured in two freshwater macroinvertebrate species using the four electrode impedance conversion technique. Specimens ofGammarus pulex (L.) andHydropsycho angustipennis (Curtis) were caught from reference streams and exposed to water from above and below the factory and to reference water for about 1 h with different kinds of behaviour being registered on-line every 10 min, such as time spent on locomotion, number of “high swimming peaks”, number of activity phases, cleaning, time spent on ventilation, ventilation frequency and netspinning. Additionally, G.pulex was exposedin situ above and 100 m, 500 m and 1000 m below the factory for 24 h. G.pulex reacted to water pollution within 1 h with less time spent on locomotion, fewer “high swimming peaks” and lower number of activity phases (p < 0.01). The same results were found after thein situ exposure (p < 0.05) and the pollution gradient mirrored survival and behavioural performance of the organisms. H. angustipennis reacted to water pollution within 1 h with decreased time spent on ventilation (p < 0.001), no change in locomotion and netspinning during daytime. During the night, exposure to water pollution resulted in increased locomotion (p < 0.0001). Simultaneous multispecies on-line biomonitoring of industrial effluents is recommended for reliable risk assessment.     

Suitability of Crangonyx pseudogracilis (Crustacea: Amphipoda) as an Early Warning Indicator in the Multispecies Freshwater Biomonitor (9 pp)

Background Biological monitors are increasingly important in 'Biological Early Warning Systems' (BEWS) for monitoring water quality. This study examines the freshwater amphipod Crangonyx pseudogracilis as a potential new indicator species when used in the Multispecies Freshwater Biomonitor (MFB). The MFB is an online continuous biomonitor which uses impedance conversion to record behavioural responses of vertebrates and invertebrates. Methods Four experiments were undertaken to establish: (1) if the electrical field generated by the MFB affected the organisms' behaviour, (2) if defined behaviours and their response to a gradient of ammonium chloride could be detected by the MFB, (3) if there was variation in the behaviour of C. pseudogracilis over a diel cycle, and (4) if behaviour changed significantly in response to a pulse of ammonium chloride. Results and Discussion Results showed no significant effect of the MFB's current on behaviour of C. pseudogracilis. Four behaviours; swimming, walking, grooming and inactivity, were observed and identified in the MFB. In the MFB, each behaviour changed significantly in response to an increasing gradient of ammonium chloride exposure. The MFB also detected increases in nocturnal activity by C. pseudogracilis. The MFB also detected a significant increase in activity after a pulse of ammonium chloride. Conclusion The range of behaviours exhibited by Crangonyx pseudogracilis, together with its ease of culture, suggest future potential of this species as an indicator species for the Multispecies Freshwater Biomonitor. Recommendations and Perspective . Further testing is required over a range of toxicants and concentration gradients to establish threshold responses and the full compliment of behaviours that could be useful in online biomonitoring.     

Why do <Emphasis Type="Italic">Manduca sexta</Emphasis> feed from white flowers? Innate and learnt colour preferences in a hawkmoth

Flower colour is an important signal used by flowering plants to attract pollinators. Many anthophilous insects have an innate colour preference that is displayed during their first foraging bouts and which could help them locate their first nectar reward. Nevertheless, learning capabilities allow insects to switch their colour preferences with experience and thus, to track variation in floral nectar availability. Manduca sexta, a crepuscular hawkmoth widely studied as a model system for sensory physiology and behaviour, visits mostly white, night-blooming flowers lacking UV reflectance throughout its range in the Americas. Nevertheless, the spectral sensitivity of the feeding behaviour of naïve moths shows a narrow peak around 450 nm wavelengths, suggesting an innate preference for the colour blue. Under more natural conditions (i.e. broader wavelength reflectance) than in previous studies, we used dual choice experiments with blue- and white-coloured feeders to investigate the innate preference of naïve moths and trained different groups to each colour to evaluate their learning capabilities. We confirmed the innate preference of M. sexta for blue and found that these moths were able to switch colour preferences after training experience. These results unequivocally demonstrate that M. sexta moths innately prefer blue when presented against white flower models and offer novel experimental evidence supporting the hypothesis that learning capabilities could be involved in their foraging preferences, including their widely observed attraction to white flowers in nature.