Quantitative PCR analysis of house dust can reveal abnormal mold conditions

Indoor mold concentrations were measured in the dust of moldy homes (MH) and reference homes (RH) by quantitative PCR (QPCR) assays for 82 species or related groups of species (assay groups). About 70% of the species and groups were never or only rarely detected. The ratios (MH geometric mean : RH geometric mean) for 6 commonly detected species (Aspergillus ochraceus, A. penicillioides, A. unguis, A. versicolor, Eurotium group, and Cladosporium sphaerospermum) were >1 (Group I). Logistic regression analysis of the sum of the logs of the concentrations of Group I species resulted in a 95% probability for separating MH from RH. These results suggest that it may be possible to evaluate whether a home has an abnormal mold condition by quantifying a limited number of mold species in a dust sample. Also, four common species of Aspergillus were quantified by standard culturing procedures and their concentrations compared to QPCR results. Culturing underestimated the concentrations of these four species by 2 to 3 orders of magnitude compared to QPCR.     

Planning Tools for Developing Comprehensive Regional Monitoring Programs

We present a planning framework that structures thelarge amount of interrelated information involved indeveloping regional monitoring programs. By regionalwe mean programs that recognize the importance ofprocesses that occur on larger geographic scales andthat cut across more than one ecosystem component. Theframework helps visualize functional relationshipsamong qualitative ingredients such as public concernsand more concrete details such as individual dataelements. It portrays the flow of information amongthe components of a regional program and ties eachaspect of the program to management decision-makingneeds. This makes it easier, during the planningprocess, to ensure that monitoring will actuallyproduce useful information. Further, the frameworksnovel, graphically oriented, and readily accessibleformat makes key information equally available to thewide range of participants (regulators, scientists,managers, public) whose input and support arenecessary for program success.     

Prenatal application of fluorescent in situ hybridization (FISH) for identification of a mosaic Y-chromosome marker,IDIC(Yp)

An amniocentesis was performed at 13.3 weeks' gestation for advanced maternal age. A mosaic sex chromosome pattern was found: of 50 cells examined, 34 had a 45,X karyotype. In 14 cells with a modal number of 46, a recognizable Y was substituted by a small non-fluorescent marker. C-banding identified the marker as an isodicentric in 12 cells. In two cells, the non-fluorescent marker appeared to be monocentric and looked like a non-fluorescent del (Yq), but could have been an isodicentric Y with inactivation of one of the centromeres. Two cells with a modal number of 47 showed two copies of the monocentric marker. Fluorescent in situ hybridization with an alpha satellite Y-specific centromeric probe confirmed the Y-chromosome origin of the markers and allowed for more accurate prenatal diagnostic information.     

Identification and field bioassays of the sex pheromone of the yellow-legged clearwing Synanthedon vespiformis (Lepidoptera: Sesiidae)

The yellow-legged clearwing (YLC) Synanthedon vespiformis (Lepidoptera: Sesiidae) occurs in the Mediterranean and central Europe. It is polyphagous, boring into the woody parts of broadleaf species including forest trees as well as various Rosaceae species. S. vespiformis has been reported as an economically important pest causing severe injury to stone fruit plantations. Many attractants for sesiid species were discovered by random field screening using 2,13- and 3,13-octadecadienyl alcohols, acetates and aldehydes, including one for S. vespiformis; and about 20 sex pheromones of sesiids have been identified so far. In the present study we identified the natural composition of the sex pheromone of YLC laboratory reared females as a blend of E3,Z13- and Z3,Z13-octadecadienyl acetates, at a ratio of 4:1. We developed an efficient lure for monitoring the pest. Pheromone funnel traps with rubber septa, impregnated with 1 mg pheromone blend, efficiently captured males for 10 weeks. Suspension of Shin-Etsu^? ropes containing a 2:1 blend of E3,Z13-18:Ac and Z3,Z13-18:Ac at 13.74 mg/ha/h, resulted in shutdown of trap catches in the treated plots and closely situated neighboring plots indicating that mating disruption is feasible.     

Use of geographic information systems for assessing groundwater pollution potential by pesticides in Central Thailand

This study employed geographic information systems (GIS) technology to evaluate the vulnerability of groundwater to pesticide pollution in Thailand. The study area included three provinces, Kanchana Buri, Ratcha Buri, and Suphan Buri, located in west-central Thailand. Factors used for the vulnerability assessment included soil texture, slope, land use, well depth, and rainfall. These vulnerability factors were reclassified to a common scale, and a weighted average was computed to yield a vulnerability score. Vulnerability factors and weights were assigned considering pesticide concentrations in 90 wells throughout the study area. Well depth was the most significant vulnerability factor. Groundwater vulnerability maps were generated for several pesticides. The eastern, agricultural part of the study area has relatively deep wells and fine soils. Shallow wells are present in the mountainous west; however, fewer pesticides are applied in that region. Consequently, much of the study area had a medium groundwater vulnerability rating, although there were pockets of high vulnerability, for example, in agricultural areas with shallow wells. The groundwater vulnerability maps are effective for identifying locations warranting more detailed groundwater pollution and vulnerability investigations.     

Mitigating urban sprawl effects: a collaborative tree and shade intervention in Phoenix,Arizona, USA

Communities in Phoenix are confronted with numerous challenges that adversely affect human health and safety, with disproportionate impacts on low-income communities. While some challenges are being addressed at the city level, new alliances at the neighbourhood level are initiating community development programmes and projects. This article reports on an intervention study carried out in collaboration with community representatives, city staff, and non-profit organisations to mitigate adverse effects of urban sprawl in the Sky Harbour Neighbourhood in Phoenix. Participatory research was conducted to design and test a tree and shade intervention. Challenges associated with navigating community desires and broader principles of sustainable development are discussed. The study offers a replicable and adaptable intervention research design aimed at empowering communities to meet urban challenges.     

Vitalizing effects of being outdoors and in nature

Five studies utilizing survey, experimental, and diary methods assessed the effects of being outdoors on subjective vitality. In Study 1, we used a vignette method to examine whether being outdoors was associated with vitality, above and beyond the influences of physical activity and social interactions. Study 2 explored the effects of being outdoors on vitality through an experimental design contrasting indoor and outdoor walks. In Study 3, participants were exposed to photographic scenes of either nature or buildings. Results showed that only the nature scenes enhanced subjective vitality. Studies 4 and 5 used a diary methodology to examine within-person variations in subjective energy as a function of being outdoors, again controlling for physical and social activity. Being outdoors was associated with greater vitality, a relation that was mediated by the presence of natural elements. Limitations of these studies are discussed, as well as their implications for research on energy and vitalization.     

A COMPARISON OF STATISTICAL TESTS ON THE EXTREME-VALUE TYPE-1 DISTRIBUTION1

ABSTRACT: The effects of the selections of plotting position formulae and class division schemes on goodness of fit tests are investigated for the extreme-value type-1 distribution using annual flood and annual maximum daily rainfall data. It was found that the plotting position formulae have a minor influence on the tests which involve their use, while the class division schemes may have a pronounced effect on the X²-test. The study also recommended the maximum likelihood method for fitting purposes and a new test which is independent of the afore-mentioned selections for judging the goodness of fit.     

Non-mosaic trisomy 20 presenting at 21 weeks' gestation as a thoraco-abdominal mass

Non-mosaic trisomy 20 is rare in fetuses surviving beyond the first trimester. We report a case of a fetus with non-mosaic trisomy 20 in amniotic fluid cultures obtained during the prenatal evaluation of an unusual thoraco-abdominal mass which was found at autopsy to be pulmonary sequestration. Gross inspection and autopsy of the fetus revealed multiple anomalies. Copyright © 2001 John Wiley & Sons, Ltd.