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A highly sensitive competitive ELISA was standardized to determine aflatoxin M1 (AFM1) in milk and dairy samples randomly collected from Faisalabad city, using Riedel-de-Haen, Aflatoxin M1 ELISA Systems, (Art–No. 45169). Standards of 5, 10, 25, 50, and 100 ng L?1 AFM1 in sample/standard buffer were used in optimizing anti-AFM1 antibodies and peroxidase–AFM1 conjugate. Linear standard curve showed increase in concentration (log C) accompanied by decrease in maximal absorbance (%B/B0) values (%B/B0 = 124.3 ? 45.0 log C) with highly significant negative correlation (?0.973). The middle of the test (50% B/B0) was at 30 ng L?1 with 6.7 ng L?1 the lowest detection limit (90% B/B0). Duplicate analysis of 41 samples showed AFM1 levels below EC permissible limits (50 ng L?1) with maximums of 40, 13, and 7.4 ng L?1 in milk, yogurt and butter, respectively. However, possibility of exceeding limits cannot be excluded, and demands regular monitoring. 相似文献
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Uzma Maqbool Anwar-Ul-Haq M. Jamil Qureshi 《Journal of environmental science and health. Part. B》2013,48(3):224-230
In-house developed ELISA was standardized to monitor atrazine residues in different environmental samples. The standard curve was linear, indicating an increase in log concentration with decrease in absorbance (%B/B0 = 1.075–0.042 Log C; r = ?0.966). The middle of the test was at 75 ng/L and the lowest detection limit at 4 ng/L. ELISA significantly correlated with the high performance liquid chromatography (HPLC) (r = 0.990). Internal validation showed good accuracy and precision. Maximum atrazine residues were present in Jehlum River water/sediments and maize/sugarcane plant roots. Most of the food samples were found to be contaminated. ELISA required less clean-up steps than HPLC, but showed matrix effect in soil/colored extracts. 相似文献
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