pH-gradient real-time aeration control for nitritation community selection in a non-porous hollow fiber membrane biofilm reactor (MBfR) with dilute wastewater

Nitritation (ammonium to nitrite) as a pre-treatment of Anammox (anaerobic ammonium oxidation) is a key step for an energy-efficient nitrogen-removal alternative from dilute wastewaters, e.g. anaerobically-treated sewage, with which limited study has achieved sustainable nitritation at ambient temperature and short hydraulic retention times. To this end, pH-gradient real-time aeration control in an oxygen-based membrane biofilm reactor was observed at 20 °C in the sequencing batch mode. An optimum oxygen supply via diffusion for ammonium-oxidizing bacteria (AOB) was established, but nitrite-oxidizing bacteria (NOB) could be inhibited. The system achieved nitrite accumulation efficiencies varying from 88% to 94% with the aeration control. Mass balance and rate performance analyses indicate that this aeration control is able to supply an oxygen rate of 1.5 mol O₂ mol^?1 ammonium fed, the benchmark oxygenation rate based on stoichiometry for nitritation community selection. Microbial analyses confirmed AOB prevalence with NOB inhibition under this aeration control.     

The significance of temperature,salinity and zinc as lethal factors for the mussel Mytilus edulis in a polluted estuary

Mussels, Mytilus edulis L., were subjected to high temperatures, low salinities and dissolved zinc in order to investigate possible environmental hazards of a discharge of heated effluent near Newport on the Yarra River estuary, Victoria, Australia. Exposure to zinc at 0.8 mg l^-1 for 14 d in otherwise favourable conditions significantly increased mortality resulting from subsequent exposure to temperatures between 29° to 31°C for 24 h without added zinc. Mussels collected from water of temporarily lowered salinity (8–16 S) showed significantly lower thermal resistance than controls collected from marine salinities (35 S). Mussels taken from a marine environment and exposed to 10 S died at a rate which increased with temperature. Mussels acclimated for 14 d to combinations of 10°, 16° and 22°C and 22 and 35 S, and subsequently exposed to increased zinc concentrations accumulated zinc to levels which were independent of temperature and salinity. The zinc was lethal more quickly at 22°C and 35 S than at the lower temperatures and salinities. The modes of toxic action of the salinity, zinc and temperature factors are discussed and it is argued that zinc which has been found accumulated in mussels near Newport could be reducing their resistance to raised temperatures and perhaps other stresses, probably as a result of effects on lysosomal functioning. The evidence suggests that the heated effluent will accelerate any toxic effects of zinc or low salinities which occur near Newport and so poses a hazard in winter as well as in summer.     

Prevalence of Epidermal Conditions in California Coastal Bottlenose Dolphins (<Emphasis Type="Italic">Tursiops truncatus</Emphasis>) in Monterey Bay

The prevalence of epidermal conditions in a small population of coastal bottlenose dolphins (Tursiops truncatus) in Monterey Bay was evaluated between 2006 and 2008. Five different skin condition categories were considered, including Pox-Like Lesions, Discoloration, Orange Film, Polygon Lesions, and Miscellaneous Markings. Of 147 adults and 42 calves photographically examined, at least 90 and 71%, respectively, were affected by at least one or multiple conditions. Pox-Like Lesions were the most prevalent, affecting 80% of the population, including adults and calves. This condition warrants the most urgent investigation being possibly indicative of the widespread presence of poxvirus or a similar pathogen in the population. In view of the high number of individuals affected, standard monitoring of the health status of Monterey Bay bottlenose dolphins is considered imperative. Discoloration was strongly associated with Pox-Like lesions. Orange Films were likely an epifaunal infestation caused by diatoms, which have been documented in other cetacean species. Polygon Lesions, a newly described category, could be the result of infestation by barnacles of the genus Cryptolepas. Miscellaneous Markings were variable in appearance and may not have the same causative factor. Although none of the proposed etiologies can be confirmed without appropriate clinical tests, recognizing common visible characteristics of the conditions could aid in preliminary comparisons across populations and individuals.     

"超级杂草"伴随着转基因作物而出现

最初的转基因大豆被基因工程业誉为“万金油”。它们能够在有除草剂的环境下生长，从理论上说，这将导致田地里那些不受欢迎的植物(比如杂草)的末日。这也将减少种植费用和除草剂的使用量。然而，多年来转基因技术的反对者们的预言现在已经变成了事实：随着时间的推移，“杂草”与转基因植物一样也具备了耐除草剂的特性。这便意味着必须应用其它除草剂，带来更多的花费。     

Human Influences on Water Quality in Great Lakes Coastal Wetlands

A better understanding of relationships between human activities and water chemistry is needed to identify and manage sources of anthropogenic stress in Great Lakes coastal wetlands. The objective of the study described in this article was to characterize relationships between water chemistry and multiple classes of human activity (agriculture, population and development, point source pollution, and atmospheric deposition). We also evaluated the influence of geomorphology and biogeographic factors on stressor-water quality relationships. We collected water chemistry data from 98 coastal wetlands distributed along the United States shoreline of the Laurentian Great Lakes and GIS-based stressor data from the associated drainage basin to examine stressor-water quality relationships. The sampling captured broad ranges (1.5–2 orders of magnitude) in total phosphorus (TP), total nitrogen (TN), dissolved inorganic nitrogen (DIN), total suspended solids (TSS), chlorophyll a (Chl a), and chloride; concentrations were strongly correlated with stressor metrics. Hierarchical partitioning and all-subsets regression analyses were used to evaluate the independent influence of different stressor classes on water quality and to identify best predictive models. Results showed that all categories of stress influenced water quality and that the relative influence of different classes of disturbance varied among water quality parameters. Chloride exhibited the strongest relationships with stressors followed in order by TN, Chl a, TP, TSS, and DIN. In general, coarse scale classification of wetlands by morphology (three wetland classes: riverine, protected, open coastal) and biogeography (two ecoprovinces: Eastern Broadleaf Forest [EBF] and Laurentian Mixed Forest [LMF]) did not improve predictive models. This study provides strong evidence of the link between water chemistry and human stress in Great Lakes coastal wetlands and can be used to inform management efforts to improve water quality in Great Lakes coastal ecosystems.     

Comparison of Observed and Predicted Sector-Averaged Air Concentrations for Elevated Releases of Fluorescein Particles

The DWNWND gaussian plume computer code was used to calculate normalized 22.5° sector-averaged dispersant concentrations at distances from 400 to 7000 meters downwind from a 56 m high release point. These results were compared to sector average values obtained in fluorescein release experiments performed at Hanford, WA between 1967 and 1973. Pasquill-Gifford (PG) and Brookhaven National Laboratory (BNL) dispersion parameterization schemes were used, and atmospheric stability classes were determined from temperature gradient (Δr/ΔZ) and wind direction standard deviation (σ_θ) measurements. Comparisons between calculated values and experimental results were made as functions of changes in dispersion parameterization, stability class determination method, deposition velocity v _d , and gravitational fall velocity v _g . Values of the correlation coefficient ranged from significant (p < 0.01) to insignificant (p > 0.05). Best agreement between comparisons was found with v _g = v _d = 1.8 cm/s and Pasquill-Gifford parameters based on stability classes determined using wind direction standard deviation, σ_θ, rather than temperature gradient, ΔT/ΔZ. The best agreement gave a Pearson’s correlation coefficient r = 0.41 (significant at p < 0.01).     

WATER QUALITY MODEL OUTPUT UNCERTAINTY AS AFFECTED BY SPATIAL RESOLUTION OF INPUT DATA1

ABSTRACT: Resolution of the input GIS data used to parameterize distributed‐parameter hydrologic/water quality models may affect uncertainty in model outputs and impact the subsequent application of model results in watershed management. In this study we evaluated the impact of varying spatial resolutions of DEM, land use, and soil data (30 × 30 m, 100 × 100 m, 150 × 150 m, 200 × 200 m, 300 × 300 m, 500 × 500 m, and 1,000 × 1,000 m) on the uncertainty of SWAT predicted flow, sediment, NO₃‐N, and TP transport. Inputs included measured hydrologic, meteorological, and watershed characteristics as well as water quality data from the Moores Creek watershed in Washington County, Arkansas. The SWAT model output was most affected by input DEM data resolution. A coarser DEM data resolution resulted in decreased representation of watershed area and slope and increased slope length. Distribution of pasture, forest, and urban areas within the watershed was significantly affected at coarser resolution of land use and resulted in significant uncertainty in predicted sediment, NO₃‐N, and TP output. Soils data resolution had no significant effect on flow and NO₃‐N predictions; however, sediment was overpredicted by 26 percent, and TP was underpredicted by 26 percent at 1,000 m resolution. This may be due to change in relative distribution of various hydrologic soils groups (HSGs) in the watershed. Minimum resolution for input GIS data to achieve less than 10 percent model output error depended upon the output variable of interest. For flow, sediment, NO₃‐N, and TP predictions, minimum DEM data resolution should range from 30 to 300 m, whereas minimum land use and soils data resolution should range from 300 to 500 m.     

Screening loblolly pine seedling responses to SO2 and O3: analysis of families differing in resistance to fusiform rust disease

Open pollinated families of loblolly pine differing in resistance to fusiform rust disease were screened in laboratory studies for responses to gaseous air pollutants. Twenty families were given acute exposures (2 fumigations for 4 h each) to SO(2) (0.4-1.0 ppm), O(3) (0.25 ppm), SO(2) (0.4-1.0 ppm) + O(3) (0.25 ppm) and control. Analyses of variance were performed to evaluate the treatment effects of these air pollutants on percent foliar injury, and to determine whether the families responded differentially to the air pollution treatments. Treatment effects were significant, with the combination treatment of SO(2) + O(3) producing a higher percentage of foliar injury than the controls; however, injury levels were very low and may not be of biological significance. Subsequently, twelve families were grown in two soil types for exposure to chronic levels of SO(2) (0.06 ppm), O(3) (0.07 ppm), SO(2) (0.06 ppm) + O(3) (0.07 ppm) and control. The families were then ranked for decreased primary shoot growth, shoot dry weight, root dry weight, total plant dry weight and root/shoot ratio after exposure to air pollution treatments. Air pollution treatments as a main effect were significant for only one of five growth parameters measured, that of primary shoot growth. The main effect of family, and the interaction of family and air pollution treatments, were significant for most growth parameters measured. In general, O(3) alone and in combination with SO(2) reduced growth more than SO(2) alone. Fumigation with O(3) reduced growth of two families in comparison with control groups, whereas SO(2) alone produced decreased growth in one family and stimulated growth in three families. Treatment with O(3) alone produced higher root/shoot ratios than fumigation with charcoal-filtered air in two families. Overall, families which were fast growers under control conditions maintained their ranking after exposure to air pollution. Families producing less growth in charcoal-filtered air also produced less growth under various air pollution regimes. Results indicated that these families exhibited a high degree of resistance to air pollution injury. Growth responses of seedlings may not reflect family differences in long-term productivity. No relationship was apparent between fusiform rust resistance and growth reductions due to air pollutants.