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Shiping Gong Melita Vamberger Markus Auer Peter Praschag Uwe Fritz 《Die Naturwissenschaften》2018,105(5-6):34
Chinese softshell turtles (Pelodiscus spp.) are widely distributed, ranging from the Amur and Ussuri Rivers in the Russian Far East through the Korean Peninsula, Japan, and eastern, central, and southern China to southern Vietnam. In East and Southeast Asia, Chinese softshell turtles are traditionally exploited for food and have been farm-bred in China since the Spring and Autumn Period, more than 2400 years ago. Currently, the annual production of Pelodiscus amounts to 340,000 t in China alone. Using mitochondrial DNA (2428 bp) and five nuclear loci (3704 bp), we examined broad sampling of wild and farm-bred Pelodiscus to infer genetic and taxonomic differentiation. We discovered four previously unknown mitochondrial lineages, all from China. One lineage from Jiangxi is deeply divergent and sister to the mitochondrial lineage of Pelodiscus axenaria. The nuclear loci supported species status for P. axenaria and the new lineage from Jiangxi. Pelodiscus maackii and P. parviformis, both harboring distinct mitochondrial lineages, were not differentiated from P. sinensis in the studied nuclear markers. The same is true for two new mitochondrial lineages from Zhejiang, China, represented by only one individual each, and another new lineage from Anhui, Guangdong, Jiangxi and Zhejiang, China. However, Vietnamese turtles yielding a mitochondrial lineage clustering within P. sinensis were distinct in nuclear markers, suggesting that these populations could represent another unknown species with introgressed mitochondria. Its species status is also supported by the syntopic occurrence with P. sinensis in northern Vietnam and by morphology. In addition, we confirmed sympatry of P. axenaria and P. parviformis in Guangxi, China, and found evidence for sympatry of P. sinensis and the new putative species from Jiangxi, China. We also discovered evidence for hybridization in turtle farms and for the occurrence of alien lineages in the wild (Zhejiang, China), highlighting the risk of genetic pollution of native stock. In the face of the large-scale breeding of Pelodiscus, we claim that the long-term survival of distinct genetic lineages and species can only be assured when an upscale market segment for pure-bred softshell turtles is established, making the breeding of pure lineages lucrative for turtle farms. Our findings underline that the diversity of Pelodiscus is currently underestimated and threatened by anthropogenic admixture. We recommend mass screening of genetic and morphological variation of Chinese softshell turtles as a first step to understand and preserve their diversity. 相似文献
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Setting of larvae of the oyster Crassostrea virginica was monitored in the James River, Virginia, USA from 1963 to 1980. Setting patterns were similar in two ways to those described prior to 1960 (before the onset of the oyster pathogen Haplosporidium nelsoni (MSX) in Chesapeake Bay): (1) setting intensity (average number of spat per shell) was greater at stations in the lower than upper estuary, and (2) on the average, 60 to 80% of the total annual set at each station occurred during a 6-week period from mid-August through September. However, annual setting intensity from 1963–1980 was lower than previously recorded, and annual sets occurred as a series of discrete pulses rather than continuously throughout the season. Pulses were each approximately 1 to 2 weeks in duration and separated by a period of diminished or no setting. Cross-correlation analysis of annual setting patterns among stations revealed three zones in the James River: the upper estuary and entire southwest side, the lower estuary, and a mid-estuary transition zone. Setting pulses tended to be synchronous at stations within each zone, but occurred 1 to 2 weeks later at stations in downriver than in upriver zones. The location of zones is related to known aspects of water circulation in the James River estuary. Moreover, pulse setting itself may be related to the absence of strong vertical salinity gradients accompanying the fortnightly stratification-destratification process.Contribution No. 1213 from the Virginia Institute of Marine Science, School of Marine Science, The College of William and Mary 相似文献
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Fritz Nüßlein Ernst Anton Feicht Sigurd Schulte-Hostede Antonius Kettrup 《Environmental Sciences Europe》1997,9(3):136-142
A site contaminated by mercury serves as an example for evaluating the environmental medicine aspects of mercury emissions on individuals living in the surroundings. Based on defined, conventional scenarios, estimations of internal exposure were made for seven different age groups and with regard to such contact media as soil, air and food. Using human monitoring methods, an attempt was made to compare the epidemiological results. The site-specific and pathway-specific estimation of exposure is based on a multitude of various samples as well as on measurements of concentrations in the compartments relevant for intake. As based upon rigid guideline values, both these calculations and the epidemiological investigations reveal a slight increase in the mercury exposure. 相似文献
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Christopher J. Yahnke Warren E. Johnson Eli Geffen Deborah Smith Fritz Hertel Michael S. Roy Cristian F. Bonacic Todd K. Fuller Blaire Van Valkenburgh Robert K. Wayne 《Conservation biology》1996,10(2):366-375
The temperate rain forest of Chiloé Island, Chile, is inhabited by an endemic fox ( Dusicyon fulvipes ) first described by Charles Darwin and now designated Darwin's fox. Despite morphological differences, Darwin's fox has been considered only an insular subspecies of the mainland chilla fox ( D. griseus ). This follows the assumption that the island population, with an estimated population of less than 500, has been separated from the mainland chilla fox for only about 15,000 years and may have received occasional immigrants from the mainland. Consequently, this island population has not been protected as endangered or bred in captivity. Recently, a population of Darwin's fox was discovered on the Chilean mainland 600 km north of Chiloé Island. This population exists in sympatry with chilla and possibly culpeo ( D. culpaeus ) foxes, which suggests that Darwin's fox may be reproductively isolated. To clarify the phylogenetic position of Darwin's fox, we analyzed 344 bp of mitochondrial DNA control-region sequence of the three species of Chilean foxes. Darwin's foxes from the island and mainland populations compose a monophyletic group distinct from the two other Chilean fox species. This indicates that Darwin's fox was probably an early inhabitant of central Chile, and that its present distribution on the mainland may be a relict of a once much wider distribution. Our results highlight the ability of molecular genetic techniques to uncover historical relationships masked by recent events, such as local extinctions. The "rediscovery" of Darwin's fox as a distinct species implies that greater significance should be given to the protection of this species and its unique habitat and to documenting the extent of its mainland distribution. 相似文献