Some toxicological aspects op methamidophos exposure in mice 1

Abstract

Cholinesterase activity in the brain, RBC and plasma of Swiss mice was determined following different routes of administration of methamidophos. Continuous feeding with the insecticide caused a progressive inhibition of both plasma‐ and erythrocyte enzymes. The effect of methamidophos was more pronounced when applied in diet than when administered dermally or intraperitoneally. Following a single injection (i.p.) of methamidophos, the brain enzyme showed maximum inhibition 24 hr following treatment. At the appearance of tremors, the plasma and RBC‐enzymes showed considerable inhibition, the former being more inhibited. The plasma enzyme appears to be the most sensitive enzyme and may be taken as a suitable index for exposure to methamidophos.     

VILLAGE DEMONSTRATION OF BIOGAS TECHNOLOGY: AN EGYPTIAN CASE STUDY

Within the context of biogas R & D and demonstration project undertaken by the Egyptian National Resarch Centre, village demonstrations are of paramount importance. In this paper, a case study of a pioneering village demonstration is presented. Based on a preliminary sociological survey, “Al Manawat” - a traditional Egyptian village was selected for the first demonstration. After a short orientation phase through which the biogas technology was introduced to a group of village representatives, a family was selected to conduct the demonstration experiment. Concomitant with the family energy requirements, availability of digester feed materials, site characteristics and other relevant factors, a family-size unit of a modified Indian type was designed and erected by the side of the farmer's house. The digester effective volume is 9 cu.m. and is connected to both a latrine and animal shed. The unit has been successfully operated since May 1981. The highlights of the construction stage as well as the unit performance characteristics are discussed. A preliminary techno-economic appraisal is also outlined. Finally, social impacts are assessed. Initial results indicate that under the Egyptian village conditions, and particularly with the heavily subsidized fuel prices, the high saving in manure transport attained through the introduction of the biogas unit, appears to be the main benefit from the farmer's point of view. Dans le cadre de la recherche et du développement du gaz de fermentation et d'un projet pilote entrepris par le Centre national de recherche égyptien, les démonstrations faites dans les villages occupent une place prépondérante. Dans cet article, l'auteur présente le cas-type d'une démonstration pilote faite dans un village. En se basant sur une enquêue sociologique préliminaire, “Al Manawat”, qui est un village égyptien traditionnel, a été choisi comme lieu de la première démonstration. Après une courte phase d'orientation au cours de laquelle le technologie du gaz de fermentation a été présentée à un groupe de notables du village, on a choisi une famille pour effectuer la démonstration expérimentale. En même temps que l'on étudiait les besoins énergétiques de cette famille, la disponibilité de matières de base de fermentation, les caractéristiques de l'emplacement et tous autres facteurs pertinents, une unité familiale de type indien modifié a été con?ue et érigée à côté de la ferme. Le composteur, d'un volume utile de matières premières de 9 m³, est relié à la fois aux latrines et à l‘étable. Cette unité fonctionne avec satisfaction depuis le mois de mai 1981. L'article met en avant les points essentiels des étapes de la construction et les caractéristiques de rendement de l'unité de fermentation. Il esquisse une évaluation technico-économique préliminaire et fait l'estimation des retombées sociales. Les premiers résultats montrent que, dans le cadre du village égyptien et particulièrement du fait que les prix du fuel sont largement subventionnés, les économies importantes réalisées dans le transport du fumier grâce á l'introduction de l'unité de fermentation semblent étre, du point de vue du fermier, le bénéfice principal. Dentro del contexto del proyecto de I & D y demostración realizado por el Centro de Investigación Nacional de Egipto, la demostración en los villorrios es de suma importancia. Este artículo presenta un caso pionero de este tipo. Basado en encuestas sociológicas, un villorrio tradicional de Egipto, “Al Manawat” fue selectiondo para la primera demostración. Después de una fase corta de orientación en la que se introdujo la tecnologia del biogás a los representantes del villorrio, se seleccionó una familia con cuya colaboración se Ilevaria a cabo el experimento. Teniendo en cuenta las necesidades de energia de la familia, la disponibilidad de materia prima para el digestor, las caracter ísticas del lugar y otros factores importantes, se diseñó y construyó un digestor de tamaño familiar, usando un modelo Indio, al lado de la casa de la familia. El volumen efectivo del digestor es 9 metros cúbicos y está conectado a la letrina y al corral de animales. La unidad está funcionando con éxito desde Mayo de 1981. Se discuten aspectos de la etapa de construcción y las características de operación. Se hace también una evaluación técnico-económica preliminar. Finalmente se evalúa el impacto social de la experiencia. Los resultados indican que en las condiciones de los villorrios Egipcios, especialmente en condiciones de precios de combustibles altamente sub-sidiados, los grandes ahorros en el transporte de abono debido a la introducción del digestor de biogas parece ser el mayor beneficio desde el punto de vista de la familia.     

Effects of mercury graded doses on redox status,metallothionein levels and genotoxicity in the intestine of sea cucumber Holothuria forskali

Mercury (Hg) is a ubiquitous and a major environmental metal pollutant in the aquatic ecosystem. The present study was performed to evaluate the effect of mercury graded doses exposure on oxidative stress, redox status, metallothionein levels and genotoxicity in the intestine of sea cucumber Holothuria forskali. Specimens were exposed for 96?h to three concentrations of Hg (40, 80 and 160?µg/L). Exposure of H. forskali to Hg promoted oxidative stress with an increase in malondialdehyde (MDA), protein carbonyl (PCO) and advanced oxidation protein products (AOPP) levels. An increase of glutathione (GSH), vitamin C (ViteC) and non-protein thiols (NPSH) contents was also observed. Additionally, antioxidant activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) increased especially with the highest doses of Hg indicating a great defense of the antioxidant system. Our investigation revealed an increase in total Metallothionein (MT) content which was more pronounced at the sharpest concentrations of Hg. A significant decline of AChE activity was also observed. In the intestine of Hg-treated H. forskali genotoxicity was confirmed by DNA degradation.     

Congener-specific accumulation of polychlorinated biphenyls in ovarian follicular wall follows repeated exposure to PCB 126 and PCB 153. Comparison of tissue levels of PCB and biological changes

OBJECTIVE: Small (SF), medium (MF) and large (LF) preovulatory porcine follicles were isolated and incubated in an Erlenmeyer flask containing 5 ml of medium with addition of PCB 126 or PCB 153 to test differences in their accumulation in the follicular wall. METHODS; The follicles were incubated in M199 medium at 37 degrees C with constant shaking at 70 rpm, for 6 days. The media were changed every day and repeated dose 25 pg/ml of PCB 126 or 25 ng/ml of PCB 153 was added each day till 6 days of culture. Media were collected every day and frozen for steroid analysis by RIA. 24 h after the last treatment follicles were frozen for further polychlorinated biphenyls (PCB) content analysis. PCB concentrations in the follicular wall were analysed by mass spectrometry. RESULTS: 3.3%; 3.6% and 5.6% of total PCB 126 dose, and 71%; 71.4% and 30.4% of total PCB 153 dose accumulated in SF, MF and LF follicles, respectively. The accumulative effect of PCB was manifested by the disruption of estradiol (E2) secretion. In SF antiestrogenic action of PCB 126 was observed during the whole time of exposure while PCB 153 decreased E2 till 4 days of culture and then estrogenic action was observed. In MF, both these congeners decreased E2 till 5 days of exposure and then estrogenic actions were noted with the highest magnification in the case of PCB 126. In LF both PCB studied increased E2 till 3 days of exposure with the highest magnification of PCB 126, then antiestrogenic action was noted. Testosterone secretion was generally affected in a pattern oposite to that of E2 suggesting action on P450arom activity. CONCLUSION: The results of these studies demonstrated that disruption of aromatization process in the follicles following repeated exposure to both congeners is not directly correlated with the bioaccumulation or amount of PCB within the follicular wall.     

Effect of slow release nitrogenous fertilizers and biochar on growth,physiology, yield,and nitrogen use efficiency of sunflower under arid climate

Environmental Science and Pollution Research - Sunflower plants need nitrogen consistently and in higher amount for optimum growth and development. However, nitrogen use efficiency (NUE) of...     

Differential accumulation of low-chlorinated (Delor 103) and high-chlorinated (Delor 106) biphenyls in human placental tissue and opposite effects on conversion of DHEA to E2

OBJECTIVE: Polychlorinated biphenyls (PCBs) and related compounds elicit a diverse spectrum of toxic responses. Additionally, they are able to pass through the human placenta. The aim of the presented data was to compare the action of low-chlorinated (Delor 103) and (Delor 106) high-chlorinated biphenyls on placental steroidogenesis. METHODS: Explants of human placental tissue were used to test differences in PCBs accumulation and influence on placental steroidogenesis. Delor 103 or 106, were added daily for six days at a dose of 200 pg from day 0 to day 6 of culture. The media in the control and experimental groups were changed every day, and collected and frozen for steroid analysis by RIA. Determinations of PCBs of tissue and medium were analysed by GC/MS/MS. RESULTS: Delor 103 was found at a higher level in the tissue than Delor 106. The first day of exposure to Delor 103 had no effect on the conversion of dehydroepiandrosterone (DHEA) to estradiol (E2) while there was a 2-fold decrease in E2 secretion from days 3 to 6. Conversely, Delor 106 caused an immediate increase in E2 secretion, which was maintained at higher levels throughout the exposure period. CONCLUSION: Differences between the accumulation of lower chlorinated and higher chlorinated biphenyls in human placental tissue and in the properties of the congeners can have multiple effects that may intensify or counteract the effects on uterine contraction by PCBs.     

One Hundred Questions of Importance to the Conservation of Global Biological Diversity

Abstract:  We identified 100 scientific questions that, if answered, would have the greatest impact on conservation practice and policy. Representatives from 21 international organizations, regional sections and working groups of the Society for Conservation Biology, and 12 academics, from all continents except Antarctica, compiled 2291 questions of relevance to conservation of biological diversity worldwide. The questions were gathered from 761 individuals through workshops, email requests, and discussions. Voting by email to short-list questions, followed by a 2-day workshop, was used to derive the final list of 100 questions. Most of the final questions were derived through a process of modification and combination as the workshop progressed. The questions are divided into 12 sections: ecosystem functions and services, climate change, technological change, protected areas, ecosystem management and restoration, terrestrial ecosystems, marine ecosystems, freshwater ecosystems, species management, organizational systems and processes, societal context and change, and impacts of conservation interventions. We anticipate that these questions will help identify new directions for researchers and assist funders in directing funds.     

Estimating the Density of Honeybee Colonies across Their Natural Range to Fill the Gap in Pollinator Decline Censuses

Abstract: Although pollinator declines are a global biodiversity threat, the demography of the western honeybee (Apis mellifera) has not been considered by conservationists because it is biased by the activity of beekeepers. To fill this gap in pollinator decline censuses and to provide a broad picture of the current status of honeybees across their natural range, we used microsatellite genetic markers to estimate colony densities and genetic diversity at different locations in Europe, Africa, and central Asia that had different patterns of land use. Genetic diversity and colony densities were highest in South Africa and lowest in Northern Europe and were correlated with mean annual temperature. Confounding factors not related to climate, however, are also likely to influence genetic diversity and colony densities in honeybee populations. Land use showed a significantly negative influence over genetic diversity and the density of honeybee colonies over all sampling locations. In Europe honeybees sampled in nature reserves had genetic diversity and colony densities similar to those sampled in agricultural landscapes, which suggests that the former are not wild but may have come from managed hives. Other results also support this idea: putative wild bees were rare in our European samples, and the mean estimated density of honeybee colonies on the continent closely resembled the reported mean number of managed hives. Current densities of European honeybee populations are in the same range as those found in the adverse climatic conditions of the Kalahari and Saharan deserts, which suggests that beekeeping activities do not compensate for the loss of wild colonies. Our findings highlight the importance of reconsidering the conservation status of honeybees in Europe and of regarding beekeeping not only as a profitable business for producing honey, but also as an essential component of biodiversity conservation.