Field demonstration of the combined effects of absorption and evapotranspiration on septic system drainfield capacity.

Drainfields for disposal of septic tank effluents are typically designed by considering the loss of water by either upward evapotranspiration into the atmosphere or lateral and downward absorption into the adjacent soil. While this approach is appropriate for evapotranspiration systems, absorption systems allow water loss by both mechanisms. It was proposed that, in areas where high evapotranspiration rates coincide with permeable soils, drainfield sizes could be substantially reduced by accounting for both mechanisms. A two-year field demonstration was conducted to determine appropriate design criteria for areas typical of the Texas High Plains. The study consisted of evaluating the long-term acceptance rates for three different drainfield configurations: evapotranspiration only, absorption only, and combined conditions. A second field demonstration repeated the experiments for additional observation of the combined evapotranspiration and absorption and achieved similar results as the first study. The field tests indicated that the current design loading criteria may be increased by at least a factor of two for the Texas High Plains region and other Texas areas with similar soil composition and evapotranspiration rates, while still retaining a factor of safety of two.     

A comparison of results from two test methods for assessing the toxicity of aminotriazole to Selenastrum capricornutum

Two different test methods for assessing the toxicity of aminotriazole to Selenastrum capricornutum are compared. Growth medium composition is demonstrated to have a significant effect on the toxicity of aminotriazole.     

Self-fertilization and maternal enzymes in the planulae of the coral Goniastrea favulus

Electrophoretic analysis of planulae obtained from controlled breeding experiments with the coral Goniastrea favulus during spawning events in 1986 and 1987 on the Great Barrier Reef, Australia, shows that offspring are produced equally frequently by outcrossing or self-fertilization, leading to a genetically determined rate of selfing of around 50%. Field rates of selfing may be greater than this as a consequence of the limited dispersal of gametes within this species. However, the level of heterozygote depression found in one population is well below that which would be predicted for such levels of selfing. In one enzyme system, glucosephosphate isomerase, the maternal phenotype continues to be expressed in the planula larva for between 11 and 17 d after fertilization. This effect is postulated to result from a residual of maternal enzyme and RNA derived from a relatively large egg.     

Preparation of high molecular weight DNA from hermatypic corals and its use for DNA hybridization and cloning

Previous attempts to extract high molecular weight DNA from hermatypic corals have failed because the starting material was mature tissue. The following paper describes the isolation of DNA from coral sperm which is of high molecular weight, and is suitable for DNA hybridization, digestion with restriction endonucleases and cloning. Gross DNA hybridization and hybridization with cloned repetitive DNA was used to investigate relatedness amongst members of the scleractinian coral family Acroporidae. Acropora formosa and A. pulchra were found to share a common (highly homologous) DNA sequence repeat; a related but less homologous sequence was present in A. latistella, and no homologous sequence was detected in Montipora digitata. These results imply a close taxonomic relationship between A. formosa and A. pulchra, and suggest that molecular criteria of this sort will be extremely useful in coral taxonomy.     

Self- and cross-fertilization in scleractinian corals

The mating patterns of four species of hermaphroditic scleractinian coral were investigated in November 1984 at Orpheus Island on the Great Barrier Reef, Australia. Each of the species shed eggs and sperm into the water, with subsequent external development of larvae. Studies of gamete viability indicated that cross-fertilizations were possible until at least 6 h after spawning.Montipora digitata cross-fertilized exclusively,Acropora tenuis, Goniastrea aspera andG. favulus were capable of self-fertilization, but to varying extents. In all species, cross-fertilization was the dominant mating pattern.     

Genetic structure of two species of Montipora on a patch reef: conflicting results from electrophoresis and histocompatibility

Spatial patterns of graft acceptance and rejection were different for two species of the coral Montipora from Kaneohe Bay, Hawaii. Electrophoretically distinct tissues were capable of fusing, indicating that clonal identity is not necessarily inferred by acceptance of grafts. For M. dilatata, 18 of 40 cases of fusion were between dissimilar genotypes and for M. verrucosa, 3 of 7 cases. In one case, fusion occurred between genotypes which shared no alleles. A greater understanding of the genetics of invertebrate immunology is required before histocompatibility criteria can be used independently to assess population genetic structure with confidence.     

Octachlorodibenzo-p-dioxin in wood treatment materials and treated wood

The octachlorodibenzo-p-dioxin (OCDD) concentrations have been determined in aged samples of commercial pentachlorophenol (PCP), in wood protection formulations containing PCP and in wood treated with PCP as a preservative or as an anti-sapstain treatment. The concentrations of OCDD found in the various samples are within the range expected from the amount of commercial PCP initially present in the samples. In view of the known stability of OCDD the results are interpreted as indicating that the formulation, treatment and ageing processes have not led to any substantial conversion of PCP to OCDD; although one result indicates that outdoor exposure of treated samples increased the OCDD concentration by a factor of approximately 2 after $\text{3}\text{1}\text{2}$ years. Analysis of aged samples of treated wood indicate that OCDD is lost much less rapidly from the wood than PCP.     

Analysis of polychlorobiphenyls from used transformers for polychlorodibenzofurans

The results of analyses for polychlorodibenzofuran (PCDF) content in two samples of polychlorobiphenyl (PCB) from used electrical transformers are presented and discussed. In neither sample was there evidence for enhanced PCDF concentrations even though one of them had been subjected to overheating while in service.     

Polychlorodibenzo-p-dioxins and dibenzofurans in technical pentachlorophenol - results of a collaborative analytical exercise

Results of analyses of eight samples of technical pentachlorophenol conducted by three different analytical methods are presented and discussed.