Evaluating the environmental effects of the GM revolution

In the US, cradle of genetically modified crops, agricultural scientists and regulators are already tackling one environmental issue, and rushing to determine the magnitude of another.     

Policy entrepreneurs in international river basins—getting climate adaptation on the cross-border water policy agenda

In this study, we look at the role which water policy entrepreneurs play in promoting and stimulating climate adaptation measures in international river basins. In a Dutch-German case study in the Rhine delta, we explore the range of strategies that policy entrepreneurs employ in cross-border water management to effectively anchor and embed climate adaptation in the water policy debate.

We focus on climate adaptation on the local and regional scale in the Deltarhine region where increased flooding and prolonged drought periods are expected under the current climate change scenarios with a considerable impact on flood protection, agricultural activities, drinking water and ecosystem development.

We analyse the impact of policy entrepreneurs while coping with the challenging cross-border setting and dealing with structural differences in national systems such as the legal and institutional framework. It is shown that whilst the European water guidelines advocate a river basin approach across borders, the guidelines do not (yet) play a catalyst role regarding climate adaptation, and the presence and activities of policy entrepreneurs contribute in putting climate adaptation on the cross-border policy agenda.

Finally, marked differences in the presence of entrepreneurs in Germany and the Netherlands are observed for which two important complementary explanations are offered relating to contextual elements of power asymmetry and dependency as well as different policy styles and organisational cultures in both countries.

     

Temporal analysis of PM10 in Metropolitan Monterrey, México

The Monterrey Metropolitan Area (MMA) is the third largest city in Mexico. Few studies have been carried out regarding its air pollution. The aim of this study was to analyze the temporal behavior of PM10 (particulate matter < or =10 microm in aerodynamic diameter). Data reported by the "Sistema Integral de Monitoreo Ambiental" (Integrated Environmental Monitoring System) network from 2006 to 2008 were used. PM10 levels were compared among the stations by year, season, and day of week. A bootstrap technique was used to obtain subsamples to which Student's t test and ANOVA were applied. PM10 levels were high and exceeded the annual limit of 50 microg/m3 set up by the Mexican standard Norma Oficial Mexicana NOM-025-SSA1-1993. These levels could have serious health effects. The southwest zone of MMA had the highest levels of PM10 during the period studied. Winter was the most polluted season, and summer was the least polluted season. Thursday and Friday were the most polluted days, and Sunday was the least polluted day. The hours with the highest levels of PM10 were 8:00 to 10:00 a.m., whereas nighttime hours were the cleanest.     

Evaluation of concentration efficiency of the Pseudomonas aeruginosa phage PP7 in various water matrixes by different methods

Enteric viruses monitoring in surface waters requires the concentration of viruses before detection assays. The aim of this study was to evaluate different methods in terms of recovery efficiencies of bacteriophage PP7 of Pseudomonas aeruginosa, measured by real-time PCR, using it as a viral control process in water analysis. Different nucleic acid extraction methods (silica–guanidinium thiocyanate, a commercial kit (Qiagen Viral RNA Kit) and phenol–chloroform with alcohol precipitation) exhibited very low recovery efficiencies (0.08–4.18 %), being the most efficient the commercial kit used for subsequent experiments. To evaluate the efficiency of three concentration methods, PBS (as model for clean water) and water samples from rivers were seeded to reach high (HC, 10⁶ pfu ml^?1) and low concentrations (LC, 10⁴ pfu ml^?1) of PP7. Tangential ultrafiltration proved to be more efficient (50.36?±?12.91, 17.21?±?9.22 and 12.58?±?2.35 % for HC in PBS and two river samples, respectively) than adsorption–elution with negatively charged membranes (1.00?±?1.34, 2.79?±?2.62 and 0.05?±?0.08 % for HC in PBS and two river samples, respectively) and polyethylene glycol precipitation (15.95?±?7.43, 4.01?±?1.12 and 3.91?±?0.54 %, for HC in PBS and two river samples, respectively), being 3.2–50.4 times more efficient than the others for PBS and 2.7–252 times for river samples. Efficiencies also depended on the initial virus concentration and aqueous matrixes composition. In consequence, the incorporation of an internal standard like PP7 along the process is useful as a control of the water concentration procedure, the nucleic acid extraction, the presence of inhibitors and the variability of the recovery among replicas, and for the calculation of the sample limit of detection. Thus, the use of a process control, as presented here, is crucial for the accurate quantification of viral contamination.     

In vivo endocrine effects of naphthenic acids in fish

Oil pollution from various sources, including exploration, production and transportation, is a growing global concern. The highest toxicity of hydrocarbon pollutants is associated with the water-soluble phase compounds, including naphthenic acids, a known component found in all hydrocarbon deposits. Recently, naphthenic acids (NAs) have shown estrogenic and anti-androgenic effects in vitro. For this reason we investigated the potential effects of two commercial mixtures of naphthenic acids on fish in vivo, using the three-spined stickleback (Gasterosteus aculeatus) as a model species.     

Studies on oxygen toxicity after simultaneous administration of paraquat and chelate-forming agents in rats

The influence of intravenously administered mixtures of paraquat and the chelating agents CaNa₂-EDTA or dithiocarb on the toxicity of oxygen at normal pressure was studied in rats. No increase in toxic effects of the combination paraquat-oxygen was seen by additionally administered chelate-forming agents.This paper completes the investigations on oxygen toxicity after administration of various chelate-forming agents in mice¹.