EDUCATING THE RURAL PUBLIC ABOUT SAFE DRINKING WATER1

ABSTRACT: From 1984 to 1987, the Penn State Cooperative Extension Service conducted 40 safe drinking water clinics for more than 3000 rural homeowners. The clinics were developed to provide homeowners and farmers with information on managing their private water supplies. Four of the clinics were evaluated using a mail survey. By using a four part mailing, response rates of 63 to 77 percent were achieved. Based on the evaluation results of the four clinics, 11 of the 14 objectives dealing with information value, knowledge gained, and actions taken were met.     

A potential sanitary sewer overflow treatment technology: fixed-media bioreactors

Under certain conditions, sanitary sewer overflows (SSOs) containing raw wastewater may be discharged to public land and can contribute to environmental and public health issues. Although this problem has attracted the attention of local, state, and federal government and regulators, relatively little SSO abatement research has been published. This study used fixed-media bioreactors, a proven onsite technology in rural areas, to treat wet weather SSO wastewater and reduce its effects on the receiving water environment. The results of this 32-month laboratory study showed that fixed-media bioreactors, especially sand bioreactors, efficiently removed organic matter, solids, and nutrients during six-hour simulated SSO peak flows. Five-day biochemical oxygen demand (BODs) of the simulated SSO varied between 40 and 125 mg/L. The average effluent concentration of BOD5 was 13 mg/L in sand bioreactors at a hydraulic loading rate of 20.4 cm/h. In addition to having high hydraulic loadings, SSO events occur infrequently. This irregularity requires that treatment systems quickly start up and effectively treat wastewater after a period of no flow. This research found that an interval up to six months between two SSO peak flows did not affect the bioreactor performance. Based on this work, fixed-media bioreactors have the potential to reduce the effects of SSOs on the water environment by following proper design parameters and operation strategies. The pollution loading of approximately 18 g BODs/m2 x h is recommended for the efficient performance of sand bioreactors in the SSO treatment.     

EDUCATING RURAL COMMUNITY LEADERS IN WASTEWATER TREATMENT ALTERNATIVES1

ABSTRACT: Providing adequate wastewater treatment facilities for the citizens in small rural communities in the United States can cost millions of dollars. An educational program has been developed and conducted in Ohio to teach local officials about wastewater treatment alternatives. The five-session workshop covers wastewater treatment principles, needs assessment, system management, and resource gathering. The knowledge and skills gained by the 96 community leaders who participated in six workshops were measured using a pretest/pest test and surveys conducted during the workshops. From 25 percent to 100 percent of the participants increased their knowledge of treatment systems, alternatives, and relative costs. From 23 percent to 84 percent applied new skills, such as conducting a sanitary survey or examining treatment systems. The pretest also revealed previous knowledge of the participants in the areas of system maintenance and consultant qualifications.     

Circuit riding for managing small community sewage systems

Providing wastewater treatment services for small communities can be expensive, so many communities have chosen part-time or inadequately trained operators. This can result in neglect and poor plant performance. Indiana County, Pennsylvania, in the USA, has established an authority to provide operation and maintenance through a circuit riding arrangement. Two operators travel to eight plants five days a week. This has resulted in more consistent operation and maintenance at affordable rates. Homeowners pay $12.60–$18.00 and commercial customers pay $25–$150 a month. Even with regular attention, these small plants are not able to meet their effluent requirements at all times.     

Development of monoclonal antibody-based immunoassays for the analysis of bisphenol A in canned vegetables

The aim of this work was the development of monoclonal antibodies (MAbs) and highly sensitive immunoassays (ELISAs) to bisphenol A (BPA), a well-known endocrine disruptor able to migrate from the internal coating of cans to food contained inside, particularly vegetables. To produce MAbs to BPA, four synthetic compounds were conjugated to proteins and used as immunizing haptens in mice. By applying hybridoma technology, several MAbs were produced and selected. These antibodies were characterized in the conjugate-coated and in the antibody-coated formats, using both homologous and heterologous conjugates. Three indirect ELISA based on the MAbs showing the highest affinity to BPA were selected. The limit of detection of the most sensitive ELISA was 0.22 nM (0.05 ng/mL), with an I?? value of around 1 nM (0.23 ng/mL). An homologous ELISA based on the MAb BPAB-11 was applied to the simple, direct determination of BPA in the liquid portion of canned artichoke, peas, and sweet corn. Only sample dilution in an appropriate saline buffer was required to minimize matrix effects and to enter the ELISA working range. Recovery and precision of the method were evaluated by spiking the liquid portion of these cans with BPA at 20, 50, and 100 ng/mL. Coefficients of variation were below 20% in most cases. With regard to recovery, the analytical data obtained were also acceptable. This immunoassay has therefore proved its potential as a new tool for the rapid, sensitive and accurate determination of BPA in canned food.