不同碳源条件下草菇内切型纤维素酶基因（eg1）转录表达的分析

利用Northernblot方法分析了不同碳源条件下草菇切型纤维素酶基因(eg1)的表达.结果发现,在含有纤维素的液体培养基中生长10d,eg1在草菇菌丝中有高效表达;纤维二糖、α-乳糖、β-乳糖,也能诱导eg1的表达,但和纤维素相比,eg1的表达量相对较低,并且它们的诱导效应在加入这类糖12h后迅速减弱;槐糖和龙胆二糖的诱导作用非常弱.在天然稻草为基质的固体栽培料生长时,草菇eg1的表达和草菇菌丝生长与出菇相对应,在菌丝生长期(d8)可见eg1的表达,d12时菌丝已长满,表达减弱,在出菇及菇体的分化及增大期,eg1的表达量逐渐增强,在成熟期达到最高水平;表明在草菇菇体发育中需要更多碳源及能源的补充,eg1在这方面起着非常重要的作用.图4参14     

Discrepant karyotypes after second- and third-trimester combined placentacentesis/amniocentesis

Cytogenetic data are presented from a total of 1306 consecutive pregnancies with successful diagnosis obtained from both chorionic villi after short-time culture (CVS-SC) and amniotic fluid cell cultures (AC); samples had been taken simultaneously at combined placentacentesis (placental biopsy) and amniocentesis during the second (92·8 per cent) and third (7·2 per cent) trimesters. Concordant results were obtained in 1218 pregnancies with a normal karyotype and in 62 pregnancies with an aberrant fetal karyotype. Discrepant, i. e. false-positive and false-negative, results were found in 26 cases (2 per cent). From these data the accuracy of CVS-SC, defined as the proportion of all correct diagnoses, is calculated to be 98 per cent. Three non-mosaic and 14 mosaic false-positive results obtained after CVS-SC could not be confirmed by AC. Related to 1235 true normal fetal karyotypes, the specificity of CVS-SC, i.e. the proportion of normal karyotypes correctly diagnosed, amounts to 98·6 per cent. In nine pregnancies, an aberrant fetal karyotype detected after AC was missed by CVS-SC. The sensitivity of CVS-SC, i.e. the proportion of abnormal fetuses correctly diagnosed (62 out of 71), amounts to 87·3 per cent in our study group.     

Investigations into the PCDD contamination of topsoil,river sediments and kaolinite clay in Queensland,Australia

Recent findings of elevated PCDDs from an unknown source in the coastal marine environment of Queensland, Australia has instigated further investigations into the distribution of, and environments associated with the PCDD contamination. This study presents data for OCDD concentrations in the coastal, mountainous and inland environment of Queensland. Additionally, full 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofuran (PCDD/F) profiles from different land-use types and environments in the coastal region were analysed. Distinct east–west gradients were detected in topsoil collected from various bushland regions with elevated OCDD concentrations confined to the coastal region. However, PCDD/F results from topsoil and river sediments collected in the Queensland coastal region suggest that elevated OCDD concentrations cannot be attributed to any of the environments, land-use or industry types investigated. PCDD/F congener profiles from select samples were remarkably similar to those previously described in marine sediments collected along the entire Queensland coastline. In addition, kaolinite clay samples from Queensland exhibited elevated OCDD concentrations, and PCDD/F profiles in these samples were similar to those detected in kaolinite clays elsewhere. Natural formation processes have been hypothesised as the source of elevated PCDDs in Queensland and other locations, where similar PCDD/F profiles and the general lack of anthropogenic sources are evident. This study presents additional data supporting this hypothesis and provides further information that may assist in the identification of the processes involved in the natural formation of PCDDs.     

Effects of methyl-, phenyl-, ethylmercury and mercurychlorid on immune cells of harbor seals （Phoca vitulina）

Mercury (Hg) is present in the marine environment as a natural metal often enhanced through human activities. Depending on its chemical form, Hg can cause a wide range of immunotoxic e ects. In this study, the influence of methyl-, ethyl- and phenylmercury as well as mercurychloride on immune functions was evaluated. Two parameters of cellular immunity, proliferation and mRNA cytokine expression of interleukin-2, -4, and transforming growth factor , were investigated in harbor seal lymphocytes after in vitro exposure to Hg compounds. While all Hg compounds had a suppressive e ect on proliferation, di erences between juvenile and adult seals were found. Lymphocytes from juveniles showed a higher susceptibility to the toxic e ect compared to lymphocytes from adults. Furthermore, the degree of inhibition of proliferation varied among the four Hg compounds. The organic compounds seem to be more immunotoxic than the inorganic compound. Finally, for the cytokine expression of methylmercury-incubated lymphocytes, timedependent changes were observed, but no dose-dependency was found. Marine mammals of the North Sea are burdened with Hg, and lymphocytes of harbor seals may be functionally impaired by this metal. The present in vitro study provides baseline information for future studies on the immunotoxic e ects of Hg on cellular immunity of harbor seals.     

Characterization of elemental pollution and its variations in sediments and suspended particulate matter from the River Elbe via multi-element analysis combined with chemometric data evaluation

Sediments and suspended particulate matter taken from about 100 sampling sites along the River Elbe from the source to the mouth during the period 1992 and 1998 have been analyzed for the concentrations of more than 60 elements in each sample. The analytical data were subsequently processed by means of multivariate statistics in order to characterize the charge of the River Elbe with inorganic pollutants to elucidate pollution trends. Using factor analysis 18 elements were found to be enriched by human activities. By means of cluster analysis--using the 18 anthropogenically influenced elements as variables--the sampling sites were aggregated into groups having similar element distributions. Thus, the entire stretch of the River Elbe was divided into three sections of characteristic elemental pollution. Finally, the trends in anthropogenic pollution within the characteristic regions since the early nineties were assessed. It turned out that most of the anthropogenically influenced elements showed a decrease in the upper and middle parts of the river, whereas no decrease could be verified in the estuary region.     

不同碳源条件下草菇内切型纤维素酶基因(egl)转录表达的分析

利用Northern blot方法分析了不同碳源条件下草菇內切型纤维素酶基因(eg1)的表达.结果发现,在含有纤维素的液体培养基中生长10 d,eg1在草菇菌丝中有高效表达;纤维二糖、α-乳糖、β-乳糖,也能诱导eg1的表达,但和纤维素相比,eg1的表达量相对较低,并且它们的诱导效应在加入这类糖12 h后迅速减弱;槐糖和龙胆二糖的诱导作用非常弱.在天然稻草为基质的固体栽培料生长时,草菇eg1的表达和草菇菌丝生长与出菇相对应,在菌丝生长期(d 8)可见eg1的表达,d 12时菌丝已长满,表达减弱,在出菇及菇体的分化及增大期,eg1的表达量逐渐增强,在成熟期达到最高水平;表明在草菇菇体发育中需要更多碳源及能源的补充,eg1在这方面起着非常重要的作用. 图4 参14     

Polychlorinated dibenzodioxins and dibenzofurans in butter from different states in Australia

Nine samples of butter from producers in various states of Australia were analysed for polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs). Detectable concentrations of 2,3,7,8-chlorine substituted PCDD/Fs were found in all samples. The mean PCDD/F concentration expressed as 2,3,7,8-TCDD equivalents (TEQs) was 0.19 pg TEQ g-1 fat. The highest concentration (0.46 pg TEQ g-1 fat) was observable in a sample from Victoria which is the most densely populated state. Overall the results indicate that PCDD/F concentrations in dairy products from Australia are low in comparison to the levels in dairy products of industrialized countries on the Northern Hemisphere. As expected, this study provides evidence that the environmental and consequently the human body burden of PCDD/Fs to be relatively low in Australia.     

Cytokine and acute phase protein expression in blood samples of harbour seal pups

Cytokines and acute phase proteins (APP) are important mediators of the immune system and sensitive parameters to assess the immune status of mammals. Especially in newborn animals, maturation of the immune system is important for their survival. However, only little information is available for pinnipeds. To get baseline levels and investigate the development of the immune system, cytokine and APP expression was analysed in blood samples of six and eight harbour seal pups collected at the German North Sea Coast in 2004 and 2005, respectively. Blood samples were taken at admission and after rehabilitation in the Seal Centre Friedrichskoog. mRNA expressions of interleukin (IL)-1β, 2, 4, 6, 8, 10, 12, interferon (IFN)γ, and transforming growth factor (TGF)β, as well as of APP haptoglobin (HP), heat shock protein (HSP) 70 and metallothionein (MT) 2 were analyzed using real time RT-PCR. Cytokine and APP expression varied between animals and years. However, general changes during rehabilitation of pups were present. Higher levels of pro-inflammatory cytokines IL-1β, 6, 8, and 12 were found at admission, consistent with an activated immune system, whereas the anti-inflammatory cytokine IL-4 was increased after rehabilitation, suggesting recovery from infections and maturation of the immune system during rehabilitation. This preliminary study suggests that cytokines are sensitive parameters to assess the immune system of harbour seals.