Evaluation of impact of PAH on a tropical fish, Oreochromis mossambicus using multiple biomarkers

Treatment of the widely occurring tropical cichlid, Oreochromis mossambicus with a pure polycyclic aromatic hydrocarbon (PAH), phenanthrene, induced a concentration-dependant formation of the enzyme, 7-ethoxyresorufin-O-deethylase (EROD). Concomittant increase (65-669%) in the activity of sorbitol dehydrogenase in the serum (SSDH) occurring with EROD induction denoted liver cell damage, which was more severe in fish exposed to lower concentrations (0.4-4 microg g-1) of the chemical. In O. mossambicus exposed to 25% refinery effluent liver damage associated with cell death was indicated by the twin analyses of SSDH and liver somatic index. Cell injury appeared to have occurred at low PAH concentrations due to inadequately induced phase II-related detoxification of metabolites. This was indicated by the nearly 33% higher activity of hepatic glutathione S-transferase (GST) in fish exposed to higher PAH concentrations as compared to low-exposure animals. Tilapia such as O. mossambicus were found to be eminently suited for biomonitoring in tropical coastal waters. A combination of EROD and serum sorbitol dehydrogenase activity measurements serves as an excellent tool for biomonitoring sublethal effects of PAH pollution in fish.     

Fluorides in groundwater and its impact on health

Fluoride is a naturally occurring toxic mineral present in drinking water and causes yellowing of teeth, tooth problems etc. Fluorspar, Cryolite and Fluorapatite are the naturally occurring minerals, from which fluoride finds its path to groundwater through infiltration. In the present study two groundwater samples, Station I and Station II at Hyderabad megacity, the capital of Andhra Pradesh were investigated for one year from January 2001 to December 2001. The average fluoride values were 1.37 mg/l at Station I and 0.91 mg/l at Station II. The permissible limit given by BIS (1983) 0.6-1.2 mg/l and WHO (1984) 1.5 mg/l for fluoride in drinking water. The groundwaters at Station I exceeded the limit while at Station II it was within the limits. The study indicated that fluoride content of 0.5 mg/l is sufficient to cause yellowing of teeth and dental problems.     

The hardrock minerals industry and the landscape architect

The landscape architecture profession is a source of creative thinking and planning which is relatively untapped within the mining industry in the United States of America. The landscape architect has been active in European operations for years as indicated by the four examples presented. The landscape architect can provide an important interface with mine design and engineering and environmental affairs. To initiate this interface within the United States, and increase its effectiveness, six objectives are presented and discussed.     

Chorionic villus sampling utilization following reports of a possible association with fetal limb defects

Prenatal diagnosis choices were reviewed in 473 women who presented for genetic counselling prior to 11 weeks' gestation for the indication of advanced maternal age. Group A consisted of 336 patients who were unaware of a possible association between chorionic villus sampling (CVS) and limb defects. Group B consisted of 137 patients who were provided this information. Fifty-one per cent of patients in group A and 45 per cent of patients in group B chose CVS. This difference was not significant by χ² analysis (P = 0·7). Patterns of prenatal diagnosis procedure utilization from 1987 to 1992 revealed a significant reduction in CVS utilization accompanied by a corresponding increase in amniocentesis after the association between CVS and limb defects was publicized. Referrals for CVS counselling also significantly declined. However, acceptance rates did not change for those patients who received genetic counselling. First-trimester genetic counselling, including a discussion regarding a possible association between CVS and limb defects, helps patients make informed decisions concerning prenatal diagnosis options, and, in our population, resulted in no change in CVS acceptance rates.     

Socio-economic impact management: programme design and implementation considerations

While larger mineral resource development projects offer new jobs and provide a stimulus to the economy of the area where they are sited, they may also pose problems in rural areas associated with rapid population growth. The purpose of an impact management programme is to anticipate and alleviate project effects that are generally perceived as undesirable and to enhance effects that are beneficial to site area communities. While the effective management of project effects is the end towards which all impact assessment efforts are directed, an integrated approach to impact management has been notably lacking. This paper suggests a systems framework for meeting this need by: (1) reviewing the need for impact management efforts; (2) discussing the objectives of, and considerations in, designing impact management programmes; (3) presenting a conceptual framework for, and key components of, such a programme; and (4) suggesting an approach for implementing such a system as an integral part of the project development process.     

White-tailed deer movement and distribution about surface coal mines in West Virginia,USA

Deer movements around surface coal mines were investigated in Preston Country, West Virginia, USA. Seventy-two deer crossings were counted on 29 surface mines with only 8% found on highwalls with more than 20% rock or stone. Thus, although slope was important, the percentage of dirt on the highwall determined use as a crossing location for deer. The average distance between all deer crossing was 209.7 m but the distance between the end of the highwall and the nearest highwall crossing was 248.7 m indicating reluctance of deer to cross mines when they could walk around them. Winter snow track counts showed fewer deer within 90 m of the top edge of the highwall. Four land bridges traversed the highwall and were heavily utilized by deer, especially in March, April, May, October and November. The surface mine bench was used as a fawn bedding area, but little feeding occurred on this portion of the mine.     

Increased formation of carcinogenic PAH metabolites in fish promoted by nitrite

Nitrite (NO(2)(-)), a highly reactive chemical species, accumulates in coastal waters as a result of pollution with nitrogenous waste and/or an imbalance in the bacterial processes of nitrification and denitrification. The present study probed the impact of nitrite (NO(2)(-)) on the metabolism of polycyclic aromatic hydrocarbons (PAHs) in fish. In a laboratory experiment, exposure of euryhaline fish, Oreochromis mossambicus to industrial effluents containing PAHs in the presence of NO(2)(-) enhanced the cytochrome P450-dependent biotransformation activity determined as 7-ethoxyresorufin-O-deethylase (EROD), by nearly 36% compared to the value observed in the absence of NO(2)(-) (50.2 +/- 6.74 pmol resorufin min(-1) g(-1) liver). Fixed wavelength fluorescence measurements in bile revealed maximum enhancement to have occurred in the metabolites of benzo[a]pyrene, a carcinogenic PAH. Lasting, sublethal physiological deterioration was apparent in fish exposed simultaneously to an oil refinery effluent and NO(2)(-), from the unremittingly decreasing liver somatic index, even after the withdrawal of the contaminants.     

A field test of processed oil shale reclamation strategies designed for minimal use of limited resources

Reclamation of processed oil shale disposal areas in the arid and semi-arid regions of Utah, Colorado and Wyoming faces several challenging problems. Shallow topsoil, harsh climate and limited water supply and low suitability of processed oil shale as a plant growth medium must be addressed if any reclamation plan is to be successful.A prototype reclamation plan that addressed the harsh environmental conditions was proposed early in the development of oil shale in eastern Utah. Studies to validate the reclamation plan were initiated in 1978 on a simulated oil shale disposal area at Anvil Points, Colorado.Four native shrubs, one forb and a grass, were successfully established by planting container-grown transplants into a topsoil-filled V-shaped trench. Supplemental water was provided from an adjacent water harvesting slope. The roots of the transplanted species grew into the adjacent shale from the soil trench, thus indicating their adaptability to the adverse oil shale conditions.Support for this research was provided by the White River Shale Corporation, Salt Lake City, Utah.