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Manickaraj Shairam Thirumalai Deepak Manjunath Prashanth Sekarbabu Viswanathan Jeganathan Sivasubramanian Sundaresan Lakshmikirupa Subramaniyam Rajalakshmi Jeganathan Manivannan 《Environmental science and pollution research international》2017,24(30):23825-23833
Environmental Science and Pollution Research - Environmental factors including pollution affect human health, and the unifying factor in determining toxicity and pathogenesis for a wide array of... 相似文献
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Jinxin He Jianjun Hu Diraviyam Thirumalai Ruediger Schade Enqi Du 《Journal of environmental science and health. Part. B》2016,51(1):8-13
Gentamicin (Gent) is an aminoglycoside antibiotic being used in livestock sector. Gent residues could cause some genetic disorders by nonsense mutations. This study aimed to develop IgY-based ELISA for the detection of Gent in animal products. Gent was conjugated with Bovine serum albumin (BSA) by carbodiimide method for further immunization in the laying chickens. PEG-6000 extraction method was employed to extract IgY from the egg yolk. The titer of anti-Gent-IgY attained the peak of 1:256,000 after the 5th booster immunization. Checkerboard titration confirmed that, anti-Gent IgY in 1:2,000 dilution could give an Optical Density (OD) 1.0 at 2 µg mL?1 of Gent-OVA coating concentration. IgY-based indirect competitive ELISA (Ic-ELISA) showed that, the IC50 value of anti-Gent IgY was 2.69 ng mL?1 and regression curve equation was y = ?16.27x + 56.97 (R2 = 0.95, n = 3), confirming that, the detection limit (LOD, IC10 value) was 0.01 ng mL?1. Recoveries from fresh milk, pork and chicken samples were ranged from 69.82% to 94.32%, with relative standard deviation lower than 10.88%. Our results suggested that generated anti-Gent IgY antibodies can be used in routine screening analysis of Gent residues in food samples. 相似文献
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