Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

某铀矿工人头发中总α,总β,铀,钍放射性水平的调查

本文对某铀矿职业工人和非职业工人头发中的总β、总α放射性水平和铀、钍含量进行了分析测定.结果表明,该矿工人体内铀和钍的蓄积量均在正常放射性本底水平范围内,从头发中检验未观察到工人体内铀、钍放射性水平有明显的升高.     

Gold mining in Siberia and the Far East as a source of mercury contamination of the environment

ＩｎｔｒｏｄｕｃｔｉｏｎＳｉｂｅｒｉａａｎｄｔｈｅＦａｒＥａｓｔａｒｅｔｈｅｏｌｄｅｓｔａｎｄｔｈｅｒｉｃｈｅｓｔｇｏｌｄ ｍｉｎｉｎｇａｒｅａｓｏｆＲｕｓｓｉａ .Ｉｎｔｅｎｓｉｖｅｇｏｌｄ ｍｉｎｉｎｇｈｅｒｅｓｔａｒｔｅｄｉｎｔｈｅｂｅｇｉｎｎｉｎｇｏｆｔｈｅ 19ｔｈｃｅｎｔｕｒｙ(Ｖｙａｚｅｌｓｈｃｈｉｋｏｖ ,1963) .Ｆｏｒｔｈｅｍｏｓｔｐａｒｔｇｏｌｄ ｂｅａｒｉｎｇｏｒｅｓａｎｄｓａｎｄｓｗｅｒｅｐｒｏｃｅｓｓｅｄｗｉｔｈｔｈｅｕｓｅｏｆａｍａｌｇａｍａｔｉｏｎｍｅｔｈｏｄｆｏｒｇｏｌｄｅ…     

“华夏古陆”考异

自葛利普创名“华夏古陆”以来,经过半个多世纪的论辩与考订,对其地质构造属性,至今仍然否定与肯定交迭起伏,莫衷一是,本文从推覆构造,陆地漂移等实际资料着手,加以论证,认为它是经历深刻改造和发生巨大位移的变质岩断片体,地质构造属性已非“华夏古陆”原义。     

Microwave digestion method in environmental analysis

Ｍｉｃｒｏｗａｖｅｄｉｇｅｓｔｉｏｎｍｅｔｈｏｄｉｎｅｎｖｉｒｏｎｍｅｎｔａｌａｎａｌｙｓｉｓ￥ＺｈａｎｇＹｕｅ（ＷａｄｓｗｏｒｔｈＣｅｎｔｒｅ，ＮｅｗＹｏｒｋＳｔａｔｅＤｅｐａｒｔｍｅｎｔｏｆＨｅａｌｔｈＳｃｈｏｏｌｏｆＰｕｂｌｉｃＨｅａｌｔｈ，Ｓ...     

效益型资源化啤酒废水处理技术应用及效果

本项处理技术屏弃了以往环境处理技术沿用的生产末端处理路线.根据啤酒生产工艺特点,在全面分析,平衡工艺废水的特性,数量及排放规律的基础上提出综合治理方案,着重于从废水中提取有用物质和能源,治理过程中把废水当做资源加以利用,而且具有明显低投入高增益的经济效益.装置2年来运行结果证明:核心技术——UASB 高效,实用,易操作,运行稳定居国内领先水平,其主要技术指标达到国内外同类研究的先进水平.该项技术可在啤酒行业及以粮食为原料的其它酿造行业和食品加工业中推广应用,并已被国家科委列为《国家级科技成果重点推广计划》及国家环保局最佳实用技术.     

电子设计竞赛与大学生创新能力培养

本文探讨了大学生电子设计竞赛对促进实践教学的重要作用，电子设计竞赛对大学生创新能力培养的作用，论述了高校深化实践教学改革与培养学生创新精神、创新能力的必要性。     

张恒东

原油管道储运行业地域辽阔、点多线长、易燃易爆，职业危害因素种类繁多，人员接触广泛，职业卫生 工作难度较大。笔者结合工作实际，就原油管道储运企业职业健康监护谈谈工作体会，