Limitations of Biodiversity Databases: Case Study on Seed-Plant Diversity in Tenerife, Canary Islands

Abstract:  Databases on the distribution of species can be used to describe the geographic patterns of biodiversity. Nevertheless, they have limitations. We studied three of these limitations: (1) inadequacy of raw data to describe richness patterns due to sampling bias, (2) lack of survey effort assessment (and lack of exhaustiveness in compiling data about survey effort), and (3) lack of coverage of the geographic and environmental variations that affect the distribution of organisms. We used a biodiversity database (BIOTA-Canarias) to analyze richness data from a well-known group (seed plants) in an intensively surveyed area (Tenerife Island). Observed richness and survey effort were highly correlated. Species accumulation curves could not be used to determine survey effort because data digitalization was not exhaustive, so we identified well-sampled sites based on observed richness to sampling effort ratios. We also developed a predictive model based on the data from well-sampled sites and analyzed the origin of the geographic errors in the obtained extrapolation by means of a geographically constrained cross-validation. The spatial patterns of seed-plant species richness obtained from BIOTA-Canarias data were incomplete and biased. Therefore, some improvements are needed to use this database (and many others) in biodiversity studies. We propose a protocol that includes controls on data quality, improvements on data digitalization and survey design to improve data quality, and some alternative data analysis strategies that will provide a reliable picture of biodiversity patterns.     

Pyrimethanil Residues on Table Grapes Italia after Field Treatment

Residues of the pyrimidine fungicide pyrimethanil [N-(4,6-dimethylpyrimidin-2yl)aniline] were determined in table grapes “Italia” by gas chromatography nitrogen-phosphorus detector (GC-NPD). Pesticides were extracted from grapes with ethyl acetate and hexane solution (1:1 v/v), and were analyzed without any further clean up. Pyrimethanil was confirmed by high-performance liquid chromatography (HPLC) fitted with a diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) in the select ion-monitoring mode (SIM). The residue of pyrimethanil was under the legal limit immediately after treatment, and showed a half-life time, calculated as a reaction of pseudo first order, of 12 days, with a regression coefficient of 0.9954. Recoveries from fortified grapes ranged between 90 and 113% with a maximum coefficient of variation (CV) of 11%. The calculated limits of detection and quantitation for pyrimethanil were 0.005 and 0.01 mg/kg, respectively.     

THE IMPACT OF GEOTHERMAL POWER PLANTS ON FOREST VEGETATION. A CASE STUDY AT TRAVALE (TUSCANY, CENTRAL ITALY)

A survey of the crown status of Turkey oak Quercus cerris L.) trees in the boron-rich area atTravale (Southern Tuscany, Italy) was carried outin order to evaluate the impact ofgeothermal power plants. Crown thinness was assessed andmorphological and chemical tests werecarried out on leaf samples. Leaf area and dry weight weredetermined as morphological parameters;while the chemical measurements performed included nitrogenand potassium (among the nutrients),and sulphur, boron, arsenic and mercury (among the elements ofgeothermal origin). Acute damagewas found only in the area immediately adjacent to the outletsand is the result of the action of boron;crown thinness, on the other hand, is affected by the natureof the geological substrate. High quantitiesof sulphur were found in leaves throughout the survey area,due both to the availability of this elementin the geopedological substrate and to the atmospheric inputof geothermal H2S. The leafcontent of this element correlates negatively with thedistance from the power plants. Boron andarsenic are the other geothermal origin elements most commonlyfound in plant matrices. The presenceof these two elements is associated with leaf area reductionand/or an increase of specific dry weight.The most significant impact on the environment appears to beexerted by the southernmost powerplant, where there is an unfavourable ratio between quantityof emissions and height of the chimneys.