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Zied Bouraoui Mohamed Banni Lassad Chouba Jihen Ghedira Cristelle Clerandeau Jamel Jebali Jean Francois Narbonne Hamadi Boussetta 《Environmental monitoring and assessment》2010,164(1-4):691-700
This paper aims to assess the marine environment quality along the Tunisian coasts using a statistical approach based on biomarkers response in the polychaete worms Nereis (Hediste) diversicolor. Worms were collected from six sites: Bizerta Lagoon, Gargour, Nakta, Mahres, Skhira and from Teboulba considered as a reference site. The biomarkers selected in this work were (1) the activities of cytochrome P450-dependent NADPH cytochrome c reductase (NADPH red) as phase I enzyme, (2) glutathione S-transferase as phase II enzyme and (3) the acetylcholinesterase activity as neurotoxicity marker. Oxidative stress was evaluated using catalase activity and malondialdehyde accumulation. For each biomarker, a discriminatory factor was calculated and a response index was allocated. For each site, a multi-marker pollution index was calculated as the sum of the response index of each of the five more discriminating biomarkers. The results show differences between sites compared with the reference samples. The multi-marker approach confirms that worms from Bizerta and Mahress have been submitted to highly polluted environment. Mahress shows the highest multi-marker pollution index, indicating a highly contamination status. 相似文献
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T. Larcher P. Perrichon C. Vignet M. Ledevin K. Le Menach L. Lyphout L. Landi C. Clerandeau F. Lebihanic D. Ménard T. Burgeot H. Budzinski F. Akcha J. Cachot X. Cousin 《Environmental science and pollution research international》2014,21(24):13833-13849
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All PAH fractions caused preneoplastic and neoplastic disorders in long-term-exposed animals. Target tissues were almost exclusively of epithelial origin, with the bile duct epithelium being the most susceptible to chronic exposure to all PAH fractions, and with germ cells being the second most responsive cells. Significantly higher incidences of neoplasms were observed with increasing PAH concentration and exposure duration. The most severe carcinogenic effects were induced by dietary exposure to HO compared to exposure to LO or PY (45, 30 and 7 %, respectively, after 9 to 10 months of exposure to an intermediate concentration of PAHs). In contrast, earliest carcinogenic effects were detected as soon as 3 mpf after exposure to LO, including the lowest concentration, or to PY. PAH bioactivation and genotoxicity in blood was assessed by ethoxyresorufin-O-deethylase activity quantification and comet and micronuclei assays, respectively, but none of these were positive. Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenotoxic events that impair survival and physiology of exposed fish. 相似文献
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Banni M Bouraoui Z Ghedira J Clearandeau C Jebali J Boussetta H 《Environmental monitoring and assessment》2009,155(1-4):119-128
Seven (7) sites from to the Tunisian coastal area were monitored through four seasons using several oxidative stress biomarkers including lipid peroxidation. Catalase (CAT) specific activity was determined in clam Ruditapes decussatus digestive gland. Lipid peroxidation was assessed in the same tissues using malondialdehyde (MDA) concentration, neutral lipid (NL) and lipofuscin accumulation. Results show that catalase specific activity varies between sites and fluctuates in time. The highest CAT activities were recorded in sites from Bizerta Lagoon and sites neighbouring Sfax city. Malondialdehyde accumulation in digestive gland cells seems to be very pronounced in animals sampled in summer and autumn. Digestive gland sections of clams sampled from reported heavy metal polluted sites exhibited higher volume density of residual bodies. Our results showed that the neutral lipids and lipofuscin content in clam's digestive gland are more sensitive to general stress as compared to other biomarkers, and could be used successfully in clams as early warning tools in field biomonitoring programs. 相似文献
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