Bio-monitoring for uranium using stream-side terrestrial plants and macrophytes

This study evaluated the abilities of various plant species to act as bio-monitors for environmental uranium (U) contamination. Vegetation and soil samples were collected from a U processing facility. The water-way fed from facility storm and processing effluents was the focal sample site as it represented a primary U transport mechanism. Soils and sediments from areas exposed to contamination possessed U concentrations that averaged 630 mg U kg(-1). Aquatic mosses proved to be exceptional accumulators of U with dry weight (dw) concentrations measuring as high as 12,500 mg U kg(-1) (approximately 1% of the dw mass was attributable to U). The macrophytes (Phragmites communis, Scripus fontinalis and Sagittaria latifolia) were also effective accumulators of U. In general, plant roots possessed higher concentrations of U than associated upper portions of plants. For terrestrial plants, the roots of Impatiens capensis had the highest observed levels of U accumulation (1030 mg kg(-1)), followed by the roots of Cyperus esculentus and Solidago speciosa. The concentration ratio (CR) characterized dry weight (dw) vegetative U levels relative to that in associated dw soil. The plant species that accumulated U at levels in excess of that found in the soil were: P. communis root (CR, 17.4), I. capensis root (CR, 3.1) and S. fontinalis whole plant (CR, 1.4). Seven of the highest ten CR values were found in the roots. Correlations with concentrations of other metals with U were performed, which revealed that U concentrations in the plant were strongly correlated with nickel (Ni) concentrations (correlation: 0.992; r-squared: 0.984). Uranium in plant tissue was also strongly correlated with strontium (Sr) (correlation: 0.948; r-squared: 0.899). Strontium is chemically and physically similar to calcium (Ca) and magnesium (Mg), which were also positively-correlated with U. The correlation with U and these plant nutrient minerals, including iron (Fe), suggests that active uptake mechanisms may influence plant U accumulation.     

A Sensitivity Analysis of Nitrogen Losses from Dairy Farms

International attention has focused on agricultural production systems as non-point sources of pollution affecting the quality of streams, estuaries and ground water resources. The objective of the current study was to develop a model of nitrogen management on the dairy farm, and to perform sensitivity analyses in order to determine the relative importance of manipulating herd nutrition, manure management and crop selection in reducing nitrogen (N) losses from the farm. The importance of the method of N input to the farm (purchased feed, legume fixation, inorganic fertilizer, imported manure) was investigated, and the potential to reduce N losses from dairy farms was evaluated. Nitrogen balance equations were derived, and related efficiency coefficients were set to reference values representing common management practices. Total farm N efficiency (animal product N per N input), and N losses per product N were determined for different situations by solving the set of simultaneous equations. Improvements in animal diet and management that increase the conversion of feed N to animal product by 50% would increase total farm N efficiency by 48% and reduce N losses per product by 36 to 40%. In contrast, reducing losses from manure collection, storage and application to improve the percentage of manure N that becomes available in soil by 100% would only improve total farm N efficiency by 13% and reduce total N losses by 14%. Selecting crops and management that can use soil nutrients 50% more efficiently would improve total farm efficiency by up to 59% and reduce N losses by up to 41% depending on the predominant nitrogen sources to the farm. Legume production would reduce N losses per product compared with non-legumes. There was more than a five fold difference in N losses per animal product N between the most extreme scenarios suggesting considerable opportunity to reduce N losses from dairy farms.     

Statistical evaluation of an agricultural land suitability model

The land evaluation and site assessment (LESA) method has been used extensively in the United States to assess agricultural land suitability for different localities. Despite widespread use, LESA models rarely have been evaluated in a systematic, comprehensive manner. This article discusses development of a LESA system for Hawaii, the first statewide application of the LESA methodology. The empirical model was implemented with a computerized geographic information system (GIS). The system's efficiency, ability to discriminate among land parcels, and robustness to subjective model parameter values are evaluated with statistical analyses and map overlays of GIS data. Results show great potential to simplify the original model specification, primarily through deletion of marginal site assessment factors. System output was generally insensitive to the numeric values selected for model parameters, with exception of the ratio used to combine the land evaluation (LE) and site assessment (SA) component scores. Relative supplies of the differing land attributes measured by the two components must be considered in determining an appropriate LE:SA ratio for a given area.     

Tennessee's Novice Driver Safety Project: a program to increase parental involvement

Graduated licensing has been implemented in many U.S. states to reduce teenage driver crash involvement. The goal is to introduce teenagers gradually to driving before allowing full, unrestricted licensure. Tennessee, one of the states that introduced graduated licensing, implemented a program to influence both driving by teenagers during the learner stage and restrictions imposed by parents after licensure. In addition to a standard welcome letter, booklets and reminder cards were mailed to parents of teenagers who had just obtained their learner's permits. The booklets and cards were designed to assist parents in becoming more actively involved in their children's driving experiences. The effects of three different approaches (welcome letter only, letter plus booklet, and letter plus booklet plus reminder cards) on parental involvement, teenage crashes, and teenage citations were assessed based on telephone interviews with parents. Although the parents said the booklets and reminder cards were helpful and should continue to be sent to other parents, there were no discernible effects on teenage practice driving and reported parental involvement during the learner stage. Nor was there any measurable influence on restrictions imposed by parents after licensure. It is unknown whether the standard welcome letter affected parental involvement, but overall supervised driving exceeded the state requirements by a substantial margin.     

CHANGING RAINFALL-RUNOFF RELATIONSHIPS IN THE URBANIZING PEACHTREE CREEK WATERSHED,ATLANTA, GEORGIA1

ABSTRACT: Peachtree Creek is a gaged watershed that has experienced a substantial increase in urbanization. The relationships of runoff to rainfall were studied for total annual flows, low flows, and peak flows. For each type of flow the relationship in the later, more urbanized period was compared to that in the earlier, less urbanized period. An increase in total runoff in wet years was observed as urbanization increased, but a decrease occurred during dry years. For low flows a similar decrease of runoff in dry years was found. An increase in peak runoff was observed over most of the range of precipitation. Increasing peak flows and declining low flows can be adequately explained by urban hydrologic theoryshed. which focuses on the effects of urban impervious surfaces upon direct runoff and infiltration. However, a decline of total runoff in dry years can be explained only by taking into account evapotranspiration as well. The concept of advectively assisted urban evapotranspiration, previously discovered by climatologists, is needed to explain such a loss of total runoff. Urban hydrologic theory must take into account vegetation and evapotranspiration, as well as impervious surfaces and their direct runoff, to explain the magnitude of total annual flows and low flows. Urban stormwater management should address the restoration of low flows, as well as the control of floods.     

A ten channel fibre-optic device for distributed sensing of underground hydrocarbon leakage

With millions of fuel storage tanks and oil pipelines installed around the world, there is inevitably frequent leakage of potentially hazardous hydrocarbons. As many of these installations are below ground, it can often be many years before the extent of the leak is discovered. We have previously reported the development of a sensor for the detection of such subterranean leaks, using infrared reflectometry to interrogate a hydrocarbon sensitive membrane. However, a single sensor cannot provide any information about the flow rate or direction of the leak. This paper describes the extension of the technology to a multi-channel distributed sensing system, using optical fibres capable of distributing the sensors over large subterranean areas. Results are reported from the evaluation of the device, which consisted of monitoring the movement of different hydrocarbons (gasoline, diesel and insulating oil) through a vertical sand-filled vessel.     

Gulf War agents trigger discrete transcriptional changes in human neuronal cells

Reports on Gulf War illness (GWI) implicated the use of the pesticide permethrin (PER), and the insect repellent N,N-diethyl-m-toluamide (DEET), in its etiology, as well as pyridostigmine bromide (PB), which was given as a prophylactic treatment against nerve agent exposure. Using Affymetrix microarrays the genomic response of human neuronal SHSY-5Y cells to 10 days of exposure to these agents was characterized and profiles of gene modulation unique to each treatment were demonstrated. In particular, a significantly greater impact of PER was observed compared to the other treatments. The Ingenuity Pathway Analysis knowledgebase was used to analyze the genomic datasets and attribute functional consequences to the effects of related genes, which were significantly up- or down-regulated in response to different treatments. Canonical pathways shown to be significantly modulated at the genomic level in response to exposure included cellular mechanisms, which might contribute to the clinical presentation in GWI patients and thus be targeted for further investigation as potential targets for therapeutic intervention.