Winter survival of microbial contaminants in soil:An in situ verification

The aim of the research was to evaluate, at site scale, the influence of freezing and freeze/thaw cycles on the survival of faecal coliforms and faecal enterococci in soil, in a climate change perspective. Before the winter period and during grazing, viable cells of faecal coliforms and faecal enterococci were detected only in the first 10 cm below ground, while,after the winter period and before the new seasonal grazing, a lower number of viable cells of both faecal indicators was detected only in some of the investigated soil profiles, and within the first 5 cm. Taking into consideration the results of specific investigations, we hypothesise that the non-uniform spatial distribution of grass roots within the studied soil can play an important role in influencing this phenomenon, while several abiotic factors do not play any significant role. Taking into account the local trend in the increase of air temperature, a different distribution of microbial pollution over time is expected in spring waters, in future climate scenarios. The progressive increase in air temperature will cause a progressive decrease in freeze/thaw cycles at higher altitudes, minimising cold shocks on microbial cells, and causing spring water pollution also during winter.     

Inspiring Futures for European Local Governments!

Since 1994, more than 2000 European local governments have adopted the principles of the Aalborg Charter as a basis for local sustainability action. Now, ten years later in June 2004, these local governments will gather once again in Aalborg, Denmark at the Fourth European Conference on Sustainable Cities and Towns in Europe, Inspiring Futures—Aalborg+10. The Aalborg+10 event will celebrate a decade of European local sustainability action, evaluate progress and stagnation, and, more importantly, will adopt new commitments to move from Agenda to Action. The conference will be crucial for accelerating local sustainability in Europe and to move towards Local Action. Here an overview is provided on the context against which this important conference is set and its expected outcomes.     

Winter survival of microbial contaminants in soil: An in situ verification

The aim of the research was to evaluate, at site scale, the influence of freezing and freeze/ thaw cycles on the survival of faecal coliforms and faecal enterococci in soil, in a climate change perspective. Before the winter period and during grazing, viable cells of faecal coliforms and faecal enterococci were detected only in the first 10 cm below ground, while, after the winter period and before the new seasonal grazing, a lower number of viable cells of both faecal indicators was detected only in some of the investigated soil profiles, and within the first 5 cm. Taking into consideration the results of specific investigations, we hypothesise that the non-uniform spatial distribution of grass roots within the studied soil can play an important role in influencing this phenomenon, while several abiotic factors do not play any significant role. Taking into account the local trend in the increase of air temperature, a different distribution of microbial pollution over time is expected in spring waters, in future climate scenarios. The progressive increase in air temperature will cause a progressive decrease in freeze/thaw cycles at higher altitudes, minimising cold shocks on microbial cells, and causing spring water pollution also during winter.     

The trace element content of top-soil and wild edible mushroom samples collected in Tuscany, Italy

The amount of the trace elements As, Ba, Cd, Cr, Cu, Hg, Li, Mn, Ni, Pb, Rb, Se, Sr, and Zn was measured in top soils and edible mushrooms, Boletus edulis, Macrolepiota procera, collected at five distinct green microhabitats inside the Lucca province, North-Central Italy (years 2008–2009). Results showed a top soil element content within the Italian statutory limits. Concerning the amount of mushroom elements, we observed significant species-differences obtaining higher levels of Ni, Rb, and Se in B. edulis or As, Pb, Cu in M. procera. Bioaccumulation factors (BCFs: element in mushroom/element in soil) resulted species-dependent and element-selective: in particular, B. edulis preferentially accumulated Se (BCFs varying from 14 to 153), while M. procera mainly concentrated Cu (BCFs varying from 5 to 15). As well, both species displayed between-site BCF differences. By a multivariate principal component approach, cluster analysis (CA), we could resolve two main clusters of soil element composition, corresponding to the most ecologically divergent sites. Besides, CA showed no cluster relating to element contents of B. edulis at the different collection sites, while a separation in groups was found for M. procera composition with respect to harvesting locations, suggesting uptake systems, in this saprotrophic species, sensitive to microhabitat. Regarding consumer safety, Cd, Hg, Pb levels resulted sometime relevant in present samples, never reaching values from current literature on mushrooms collected in urban-polluted areas. Our findings encourage a deeper assessment of the molecular mechanisms of metal intake by edible mushrooms, encompassing genetic biochemical and geo-ecological variables, with particular awareness to element bioavailability in soils and fungi.     

Inspiring Futures for European Local Governments!

Since 1994, more than 2000 European local governments have adopted the principles of the Aalborg Charter as a basis for local sustainability action. Now, ten years later in June 2004, these local governments will gather once again in Aalborg, Denmark at the Fourth European Conference on Sustainable Cities and Towns in Europe, Inspiring Futures—Aalborg+10. The Aalborg+10 event will celebrate a decade of European local sustainability action, evaluate progress and stagnation, and, more importantly, will adopt new commitments to move from Agenda to Action. The conference will be crucial for accelerating local sustainability in Europe and to move towards Local Action. Here an overview is provided on the context against which this important conference is set and its expected outcomes.     

Drivers of support for citizen science across state wildlife management agencies in the United States

Citizen science (CS) is gaining recognition as a valuable approach to meet data needs for environmental projects while fostering collaboration between scientists and members of the public. Despite increasing implementation of CS by natural resource entities, organizations’ motivations for engaging in CS remain poorly understood. We examined the utility of the theory of planned behavior (TPB) and social exchange theory (SET) in identifying factors influencing support of CS by scientific organizations. To test predictions of the TPB and SET theories, we surveyed (quantitative, web based) state wildlife agency staff in the United States on their perceptions of organizational engagement in CS. We divided questions that measured TPB items into individual and organizational components to address the influence of personal- and organization-level decision-making on staff perceptions and attitudes. We used structural equation modeling to identify key constructs that influence staff support of CS in state wildlife agencies. The survey yielded 627 responses across 44 states. Both TPB and SET constructs accurately predicted staff support of CS; however, measures from SET (e.g., public engagement benefits and costs of CS to scientific credibility) were most influential (i.e., TPB constructs had less impact). Our findings indicate that organizational support for CS is primarily influenced by assessment of trade-offs among perceived costs and benefits. Indicators of support for CS were further elucidated by including measures from the TPB model. Based on our results, we suggest that natural resource entities give careful consideration to CS project design, develop thorough communication and data management plans, and practice iterative evaluation of CS project productivity.     

A Screening Procedure for Prioritizing Riparian Management

A spatially explicit linear, additive model was developed for quantifying site characteristics of riparian areas of the lower Cedar River, Washington, USA. The spatial complexity and distribution of combined habitat and anthropogenic landscape features were used to define habitat indices that indicate the relative quality of riparian habitats. Patches of contiguous grid cells were measured in terms of their locations, sizes, and relative degree of fragmentation. Additionally, intrapatch heterogeneity was measured to identify unique combinations of habitat and anthropogenic factors for individual grid cells within patches. Model verification indicated that existing floodplain riparian habitats received positive indices more than 90% of the time. Mean patch sizes and fragmentation indices were similar for all positive indices throughout the reaches in the valley floor. Among all reaches, reach 7 had the highest number of positive patches due to a higher degree of meandering in this reach. This procedure and model outputs provide unique screening opportunities for prioritizing management of riparian areas (e.g., conservation, restoration and enhancement).     

Application of laser ablation ICP-MS and traditional techniques to the study of black crusts on building stones: a new methodological approach

Introduction  

In this work, we propose an innovative application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as a methodological approach for the chemical characterisation of black crusts on stone monuments, associated with traditional micro-morphological (optical and scanning electron microscopy) and infrared spectroscopic techniques (FTIR).     

Metals Associated with Suspended Sediments in Lakes Erie and Ontario, 2000–2002

Sediment traps were deployed in the three major basins of Lake Erie, and the central (Mississauga) basin of Lake Ontario, and refurbished seasonally over the period 2000–2002. In Lake Ontario, sediment down-flux rates and corresponding contaminant down-flux rates were highest in winter during periods of unstratified thermal conditions, and generally increased with depth due to the influence of resuspended bottom sediments during all sampling periods. Lake Ontario suspended sediments exhibited the highest concentrations of metals; concentrations of mercury and lead frequently exceeded guideline values for bottom sediments. Contaminant levels in Lake Ontario suspended sediments were similar to concentrations in bottom sediments in the same area. There was a spatial trend toward higher suspended sediment metals concentrations from the eastern basin to the western basin of Lake Erie, which is similar to the trend in bottom sediment contamination. In the eastern basin of Lake Erie, which is the deepest area of the lake, there was no trend in down-flux rate with depth in 2001; however, down-flux rates increased with depth in 2002. Suspended sediments in the western basin of Lake Erie were determined to be largely resuspended bottom sediments; all western basin samples collected in the study exceeded the guideline value for mercury (0.486 μg/g).     

Plant volatiles affect oviposition by codling moths

Summary. Oviposition in wild codling moth females, collected as overwintering larvae from apple, pear and walnut, was stimulated by volatiles from fruit-bearing green branches of these respective hostplants. Analysis of headspace collections showed that eight compounds present in apple, pear and walnut elicited a reliable antennal response in codling moth females: (E)--ocimene, 4,8-dimethyl-1,(E)3,7-nonatriene, (Z)3-hexenyl acetate, nonanal, -caryophyllene, germacrene D, (E,E)--farnesene, and methyl salicylate. Any one of these compounds is found in many other non-host plants, and host recognition in codling moth is thus likely encoded by a blend of volatiles. A large variation in the blend proportion of these compounds released from apple, pear and walnut suggests a considerable plasticity in the female response to host plant odours. Wild females, collected as overwintering larvae in the field, laid significantly fewer eggs in the absence of host plant volatiles. The offspring of these females, however, reared on a semi-artificial diet in the laboratory, laid as many eggs with or without plant volatile stimulus. Tests with individual females showed that this rapid change in oviposition behaviour may be explained by selection for females which oviposit in the absence of odour stimuli, rather than by preimaginal conditioning of insects when rearing them on semi-artificial diet. Oviposition bioassays using laboratory-reared females are therefore not suitable to identify the volatile compounds which stimulate egglaying in wild females.